A5101988939- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Nanofluid Flow and Heat Transfer
- Semiconductor materials and devices
- Diamond and Carbon-based Materials Research
- Advancements in Semiconductor Devices and Circuit Design
- Fractional Differential Equations Solutions
- Rheology and Fluid Dynamics Studies
- Chromosomal and Genetic Variations
- Quantum and electron transport phenomena
- High-pressure geophysics and materials
- Multiferroics and related materials
- Epigenetics and DNA Methylation
- Semiconductor Quantum Structures and Devices
- RNA Research and Splicing
- Magnetic properties of thin films
- RNA and protein synthesis mechanisms
- Supercapacitor Materials and Fabrication
- Electronic and Structural Properties of Oxides
- Fluid Dynamics and Turbulent Flows
- RNA Interference and Gene Delivery
- Ferroelectric and Piezoelectric Materials
- Semiconductor materials and interfaces
- Advanced biosensing and bioanalysis techniques
- Silicon Nanostructures and Photoluminescence
Mehran University of Engineering and Technology
2024
Wilson Community College
2024
Jamia Millia Islamia
2023-2024
Koç University
2023
University of Gujrat
2023
Université de Montpellier
2017-2021
Centre National de la Recherche Scientifique
2017-2021
Laboratoire Charles Coulomb
2017-2021
Aix-Marseille Université
2020
University of Basel
2020
Highlights•A method for parallel monitoring of thousands reporters integrated in the genome•Genome-wide landscape chromatin position effects mouse embryonic stem cells•Attenuation transcriptional activity lamina-associated domains•• Enhancers and transcription units influence gene expression generally over ∼20 kbSummaryReporter genes into genome are a powerful tool to reveal regulatory elements local context on expression. However, so far such reporter assays have been low throughput. Here,...
The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little known about the kinetics and fidelity of double-strand break (DSB) repair process that follows cutting event in living cells. Here, we developed strategy to measure DSB single loci human Quantitative modeling repaired time series after activation reveals variable often slow rates, with half-life times up ∼10 hr. Furthermore, DSBs tends be error prone. Both classical microhomology-mediated end joining pathways...
This study reports on the process optimization of ultrasound-assisted, food-grade oil-water nanoemulsions stabilized by modified starches. In this work, effects major emulsification variables including applied power in terms density and sonication time, formulation parameters, that is, surfactant type concentration, bioactive concentration dispersed-phase volume fraction were investigated mean droplet diameter, polydispersity index charge emulsion droplets. Emulsifying properties octenyl...
Abstract Antiferromagnetic thin films are currently generating considerable excitement for low dissipation magnonics and spintronics. However, while tuneable antiferromagnetic textures form the backbone of functional devices, they virtually unknown at submicron scale. Here we image a wide variety spin in multiferroic BiFeO 3 that can be tuned by strain manipulated electric fields through room-temperature magnetoelectric coupling. Using piezoresponse force microscopy scanning NV magnetometry...
The ability of retroviruses and transposons to insert their genetic material into host DNA makes them widely used tools in molecular biology, cancer research gene therapy. However, these systems have biases that may strongly affect outcomes. To address this issue, we generated very large datasets consisting unselected integrations the mouse genome for Sleeping Beauty (SB) piggyBac (PB) transposons, Mouse Mammary Tumor Virus (MMTV). We analyzed (epi)genomic features generate bias maps at both...
TATA binding protein (TBP) is a key component of the eukaryotic transcription initiation machinery. It functions in several complexes involved core promoter recognition and assembly pre-initiation complex. Through gene duplication eukaryotes have expanded their repertoire proteins, leading to variable composition In vertebrates this consists TBP, TBP-like factor (TLF, also known as TBPL1, TRF2) TBP2 (also TBPL2, TRF3). All three factors are essential, with TLF playing important roles...
EZH2 is frequently overexpressed in glioblastoma (GBM), suggesting an oncogenic function that could be a target for therapeutic intervention. However, reduced activity can also promote tumorigenesis, leading to concerns about the use of inhibitors. Here, we provide further insight effects prolonged Ezh2 inhibition using preclinical mouse models and primary tumor-derived human GBM cell lines. Using doxycycline-inducible shRNAs mimic selective inhibitor, demonstrate depletion causes robust...
Abstract Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to design effective gene editing assays. However, it challenging profile Cas9 in endogenous cellular environment. Here we leverage our TRIP technology integrate ~ 1k barcoded reporter genes genomes mouse embryonic stem cells. We target integrated reporters (IRs) using RNA-guided characterize induced mutations by sequencing. report that gRNA-sequence IR explain most variation mutation...
Since the discovery of induced pluripotent stem cells there has been intense interest in understanding mechanisms that allow a somatic cell to be reprogrammed back state. Several groups have studied alterations gene expression occur as modify their genome an embryonic cell. Underpinning many changes are modifications epigenetic profile associated chromatin. We used large-scale shRNA screen identify modifiers act barriers reprogramming. uncovered important role for TRIM28 resisting transition...
In this study, pure ZnO and Al-doped thin films were developed on glass by sol–gel process followed drying annealing in air at 170 400 °C, respectively. The surface morphology structural characteristics determined through scanning electron microscopy, atomic force microscopy X-ray diffraction. Fourier transform infrared spectroscopy validated the formation of film substrate. It was evaluated that 1 at% aluminum (Al) doping showed low electrical resistivity higher charge carrier concentration...
Antiferromagnetic materials are promising platforms for next-generation spintronics owing to their fast dynamics and high robustness against parasitic magnetic fields. However, nanoscale imaging of the order in such with zero net magnetization remains a major experimental challenge. Here we show that non-collinear antiferromagnetic spin textures can be imaged by probing noise they locally produce via thermal populations magnons. To this end, perform nanoscale, all-optical relaxometry...
Nitrogen-vacancy magnetic microscopy is employed in the quenching mode as a noninvasive, high-resolution tool to investigate morphology of isolated skyrmions ultrathin films. The skyrmion size and shape are found be strongly affected by local pinning effects field history. Micromagnetic simulations including static disorder, based on physical model grain-to-grain thickness variations, reproduce all experimental observations reveal key role disorder history stabilization This work opens way...
Genomically distal mutations can contribute to the deregulation of cancer genes by engaging in chromatin interactions. To study this, we overlay viral cancer-causing insertions obtained a murine retroviral insertional mutagenesis screen with genome-wide conformation capture data. Here find that tend cluster 3D hotspots within nucleus. The identified are significantly enriched for known genes, and bear expected characteristics bona fide regulatory interactions, such as enrichment...
In clinical trials, sample size is usually lesser as compared to other epidemiological studies make it more feasible and cost effective. Small sizes of such trials discourage the use parametric test due violation assumption under which they are applicable. Therefore, nonparametric substantial in two or independent samples. The Kruskal-Wallis h an alternative one-way ANOVA can be used identify significant differences among different populations. When we have several samples assumed arranged...
Efficient, fully deterministic current-induced generation and displacement of magnetic skyrmions (quasiparticles with topologically protected chiral spin texture) is a prerequisite for whole class spintronic devices. One the key ingredients fast dynamics material low damping, Heusler alloys are interesting in this respect. The authors use N-$V$ center magnetometry to study creation movement Co-based alloy, show that skyrmion facilitated by application an in-plane field. Pinning effects due...