A5100428795- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Nanoparticles: synthesis and applications
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Photosynthetic Processes and Mechanisms
- Microplastics and Plastic Pollution
- Microbial Fuel Cells and Bioremediation
- Food Quality and Safety Studies
- Amino Acid Enzymes and Metabolism
- Meat and Animal Product Quality
- Microbial Community Ecology and Physiology
- Evolution and Genetic Dynamics
- Fungal Biology and Applications
- Porphyrin Metabolism and Disorders
- Plant Virus Research Studies
- Plant-derived Lignans Synthesis and Bioactivity
- Traditional Chinese Medicine Analysis
Tianjin University
2016-2025
Fudan University
2021-2025
Zhejiang University
2014-2025
Wuyi University
2022-2024
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2015-2024
Dalian Polytechnic University
2024
Pudong Medical Center
2024
State Key Laboratory of Genetic Engineering
2024
Agricultural Research Organization
2024
Sichuan Agricultural University
2015-2024
The efficacy and the mutation spectrum of genome editing methods can vary substantially depending on targeted sequence. A simple, quick assay to accurately characterize quantify induced mutations is therefore needed. Here we present TIDE, a method for this purpose that requires only pair PCR reactions two standard capillary sequencing runs. sequence traces are then analyzed by specially developed decomposition algorithm identifies major in projected site determines their frequency cell...
Bacterial spot caused by Xanthomonas perforans is a major disease of tomatoes, leading to reduction in production 10-50%. While copper (Cu)-based bactericides have been used for management, most the X. strains isolated from tomatoes Florida and other locations worldwide are Cu-resistant. We developed DNA-directed silver (Ag) nanoparticles (NPs) grown on graphene oxide (GO). These Ag@dsDNA@GO composites effectively decrease cell viability culture plants. At very low concentration 16 ppm...
The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little known about the kinetics and fidelity of double-strand break (DSB) repair process that follows cutting event in living cells. Here, we developed strategy to measure DSB single loci human Quantitative modeling repaired time series after activation reveals variable often slow rates, with half-life times up ∼10 hr. Furthermore, DSBs tends be error prone. Both classical microhomology-mediated end joining pathways...
Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation desired at target site difficult to predict and therefore must be empirically determined. Here, we adapted widely used TIDE method quantification templated events, including point mutations. The resulting TIDER rapid, cheap accessible testing optimization template-directed genome strategies. A free web data analysis available http://tide.nki.nl.
The present study was designed to determine the effects of artemisinin (ARS) and its derivatives on human ovarian cancer cells, evaluate their potential as novel chemotherapeutic agents used alone or in combination with a conventional agent, investigate underlying mechanisms action. Human cells (A2780 OVCAR-3), immortalized non-tumourigenic surface epithelial (IOSE144), were exposed four ARS compounds for cytotoxicity testing. vitro vivo antitumour possible action dihydroartemisinin (DHA),...
We investigated and compared the concentration-dependent cytotoxicity of single-walled carbon nanotubes (SWCNTs) SWCNTs functionalized with polyethylene glycol (SWCNT-PEGs) in neuronal PC12 cells at biochemical, cellular, gene expressional levels. elicited a manner, SWCNT-PEGs exhibited less cytotoxic potency than uncoated SWCNTs. Reactive oxygen species (ROS) were generated both concentration- surface coating-dependent manner after exposure to these nanomaterials, indicating different...
Objective— Although early proof-of-concept studies of somatic in vivo genome editing the mouse ortholog proprotein convertase subtilisin/kexin type 9 ( Pcsk9 ) mice have established its therapeutic potential for prevention cardiovascular disease, unique nature genome-editing technology—permanent alteration genomic DNA sequences—mandates that it be tested against human genes normal cells with genomes to give reliable preclinical insights into efficacy (on-target mutagenesis) and safety (lack...
Rice (Oryza sativa L.) frequently suffers in late spring from severe damage due to cold spells, which causes the block of chlorophyll biosynthesis during early rice seedling greening. However, inhibitory mechanism by this occurs is still unclear. To explore responsive seedlings low temperatures greening, effects chilling stress on and plastid development were studied seedlings. Chlorophyll was obviously inhibited accumulation declined under The decrease synthesis δ-aminolevulinic acid (ALA)...
A synbio strategy for efficient sugar-to-electricity conversion.
Riboflavin (vitamin B2), the precursor of flavin cofactors mononucleotide (FMN) and adenine dinucleotide (FAD), is used commercially as an animal feed supplement food colorant. E. coli a robust host for various genetic manipulations has been employed efficient production biofuels, polymers, amino acids, bulk chemicals. Thus, aim this study was to understand metabolic capacity riboflavin by modification central metabolism, biosynthesis pathway optimization fermentation conditions. The basic...
Background Most cardiovascular diseases occur in low‐ and middle‐income regions of the world, but socioeconomic distribution within China remains unclear. Our study aims to investigate whether prevalence differs among high‐, middle‐, low‐income explore reasons for disparities. Methods Results We enrolled 46 285 individuals from 115 urban rural communities 12 provinces across between 2005 2009. recorded their medical histories calculated INTERHEART Risk Score assessment risk‐factor burden,...
The physicochemical characteristics of silver nanoparticles (AgNPs) may greatly alter their toxicological potential. To explore the effects size and coating on cytotoxicity genotoxicity AgNPs, six different types having three sizes two coatings, were investigated using Ames test, mouse lymphoma assay (MLA) in vitro micronucleus assay. genotoxicities acetate nitrate evaluated to compare nanosilver that ionic silver. test produced inconclusive results for all materials due high toxicity...
2,3-Butanediol (2,3-BD) with low toxicity to microbes, could be a promising alternative for biofuel production. However, most of the 2,3-BD producers are opportunistic pathogens that not suitable industrial-scale fermentation. In our previous study, wild-type Bacillus subtilis 168, as class I microorganism, was first found generate only d-(-)-2,3-BD (purity >99 %) under oxygen conditions.In this work, B. engineered produce chiral pure meso-2,3-BD. First, production abolished by deleting...
Organisms have built up immunological systems, where mitochondrial SO2 plays conflicting roles in regulating cell apoptosis. However, no exploration on the influence and principle of to specific apoptosis type can be found, which brings about a challenge fluorescent probes. Herein, we optimize fluorophore develop new probe FHMI ((E)-4-(3-formyl-4-hydroxystyryl)-1-methylpyridin-1-iumiodide) by equipping an ICT (intramolecular charge transfer) HMII...
Abstract Establishing saturated mutagenesis in a specific gene through editing is an efficient approach for identifying the relationships between mutations and corresponding phenotypes. CRISPR/Cas9-based sgRNA library screening often creates indel with multiple nucleotides. Single base editors dual deaminase-mediated can achieve only one two types of substitutions, respectively. A new glycosylase editor (CGBE) system, which uracil inhibitor (UGI) replaced uracil-DNA (UNG), was recently...