A5101671236- Bacterial Genetics and Biotechnology
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Yersinia bacterium, plague, ectoparasites research
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Transgenic Plants and Applications
- IL-33, ST2, and ILC Pathways
- Genetic factors in colorectal cancer
- HIV Research and Treatment
- Neonatal Respiratory Health Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Outbreaks Research
- Asthma and respiratory diseases
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Pneumonia and Respiratory Infections
- Helicobacter pylori-related gastroenterology studies
- Pleural and Pulmonary Diseases
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
Israel Institute for Biological Research
2013-2024
Weizmann Institute of Science
2008-2021
University of Pennsylvania
2016-2019
Monell Chemical Senses Center
2016
Philadelphia VA Medical Center
2016
Abstract Previously, we reported that a peptide, p458, from the sequence of mammalian 60-kDa heat shock protein (hsp60) molecule can serve as carrier in conjugate vaccines with capsular polysaccharide (CPS) molecules various bacteria. These were effective injected PBS without added adjuvants. We now report p458 conjugated to pneumococcal CPS type 4 (PS4) manifests innate adjuvant effects: it stimulated mouse macrophages secrete IL-12 and induced late appearance PS4 on macrophage surface...
Recent advances in the field of cell therapy have proposed new solutions for tissue repair and regeneration using various delivery approaches. Here we studied ex vivo a novel topical system encapsulated cells hybrid polyethylene glycol-fibrinogen (PEG-Fb) hydrogel microspheres to respiratory tract models. We investigated basic parameters encapsulation, release conditions inflamed damaged lungs bacterial-infected mice. The establishment each step study was essential proof concept....
HIV infection is initiated by the fusion of viral membrane with target T-cell membrane. The envelope glycoprotein, gp41, contains a peptide (FP) in N terminus that functions together other gp41 domains to fuse virion host cell We recently reported FP co-localizes CD4 and receptor (TCR) molecules, co-precipitates TCR, inhibits antigen-specific proliferation pro-inflammatory cytokine secretion. Molecular dynamic simulation implicated an interaction between α-helical transmembrane domain (TM)...
The COVID-19 pandemic has led to high global demand for vaccines safeguard public health. To that end, our institute developed a recombinant viral vector vaccine utilizing modified vesicular stomatitis virus (VSV) construct, wherein the G protein of VSV is replaced with spike SARS-CoV-2 (rVSV-ΔG-spike). Previous studies have demonstrated production VSV-based in Vero cells adsorbed on Cytodex 1 microcarriers or suspension. However, titers were limited by both carrier surface area and shear...
Mice are exceedingly sensitive to intra-peritoneal (IP) challenge with some virulent pneumococci (LD50 = 1 bacterium). To investigate how peripheral contact bacterial capsular polysaccharide (PS) antigen can induce resistance, we pulsed bone marrow dendritic cells (BMDC) of C57BL/6 mice type 4 or 3 PS, injected the BMDC intra-foot pad (IFP) and challenged IP supra-lethal doses pneumococci. We examined responses T B in draining popliteal lymph node measured effects on bacteria peritoneum...
The intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related the scarcity and small sizes ILFs their follicle-associated epithelium (FAE) impeded characterization spatial gene expression programs. Here, we combined RNA sequencing laser capture microdissected tissues single-molecule transcript imaging obtain a map ILF its associated FAE in mouse intestine. We identified zonated programs...
Abstract Constant exposure of intestinal epithelial cells (IEC) to potentially damaging stimuli makes mucosal repair programs essential for gut homeostasis, immunological quiescence and resistance colitis. Trefoil factor 2 (TFF2) is a mucus associated protein that promotes barrier integrity in the lung intestine, but whether bona-fide TFF2 receptor exists remains controversial. Herein, we provide evidence leucine rich repeat nogo interacting 3 (LINGO3) transmembrane component signaling...
Abstract The intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related the scarcity and small sizes ILFs their follicle-associated epithelium (FAE) impeded characterization spatial gene expression programs. Here, we combined RNA sequencing laser capture microdissected tissues single molecule transcript imaging obtain a map ILF its associated FAE in mouse intestine. We identified zonated...