A5053954357- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Hematopoietic Stem Cell Transplantation
- COVID-19 Clinical Research Studies
- Plant-based Medicinal Research
- Long-Term Effects of COVID-19
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Pharmacological Effects of Natural Compounds
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Sphingolipid Metabolism and Signaling
- Respiratory viral infections research
- Trypanosoma species research and implications
- Animal Virus Infections Studies
- Multiple Myeloma Research and Treatments
- Chemokine receptors and signaling
- Galectins and Cancer Biology
- Liver physiology and pathology
- Ethnobotanical and Medicinal Plants Studies
- Ubiquitin and proteasome pathways
Israel Institute for Biological Research
2014-2023
Institute for Biological Sciences
2021
Laboratory of Molecular Genetics
2021
University of Louisville
2013-2014
Weizmann Institute of Science
2007-2013
Bar-Ilan University
2007
Abstract The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of efficient and cost-effective vaccine, suitable mass immunization. Here, we show the a replication competent recombinant VSV-∆G-spike in which glycoprotein VSV is replaced spike protein SARS-CoV-2. In-vitro characterization this vaccine indicates expression presentation on viral membrane with antigenic similarity to A golden Syrian hamster in-vivo model implemented. We that single-dose...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of ongoing disease 2019 (COVID-19) pandemic. The continued spread SARS-CoV-2 increases probability influenza/SARS-CoV-2 coinfection, which may result in severe disease. In this study, we examine outcome influenza A virus (IAV) and coinfection K18-hACE2 mice. Our data indicate enhance susceptibility IAV-infected mice to developing upon with two days later. contrast nonfatal lower mortality rates due alone,...
The mechanisms governing hematopoietic progenitor cell mobilization are not fully understood. We report higher membrane type 1–MMP (MT1-MMP) and lower expression of the MT1-MMP inhibitor, reversion-inducing cysteine-rich protein with Kazal motifs (RECK), on isolated circulating human CD34+ cells compared immature BM cells. correlated clinical in healthy donors patients lymphoid malignancies. Treatment G-CSF further increased decreased RECK murine a PI3K/Akt-dependent manner, resulting...
Pneumonic plague is a fatal disease caused by Yersinia pestis that associated with delayed immune response in the lungs. Because neutrophils are first cells recruited to sites of infection, we investigated mechanisms responsible for their homing lung. During 24 hr after pulmonary infection fully virulent Y. strain, no significant changes were observed lungs levels infiltrate, expression adhesion molecules, or major neutrophil chemoattractants keratinocyte cell-derived chemokine (KC),...
In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of plague pathogen Yersinia pestis led to rapid induction protective humoral immune response via pivotal activation innate-like B1b cells. Conversely, monomeric version failed promptly protect vaccinated animals in this model bubonic plague. examined ability confer onset immunity more challenging mouse pneumonic Vaccination one dose adsorbed on aluminum hydroxide elicited effective protection against...
Abstract The COVID-19 pandemic caused by SARS-CoV-2 that emerged in December 2019 China resulted over 7.8 million infections and 430,000 deaths worldwide, imposing an urgent need for rapid development of efficient cost-effective vaccine, suitable mass immunization. Here, we generated a replication competent recombinant VSV-ΔG-spike which the glycoprotein VSV was replaced spike protein SARS-CoV-2. In vitro characterization indicated expression presentation on viral membrane with antigenic...
The COVID-19 pandemic caused by the SARS-CoV-2 infection induced lung inflammation characterized cytokine storm and fulminant immune response of both resident migrated cells, accelerating alveolar damage. In this work we identified members matrix metalloprotease (MMPs) family associated with extra-cellular (ECM) destruction using K18-hACE2-transgenic mice (K18-hACE2) infected intranasally SARS-CoV-2. Five days post infection, lungs exhibited overall damage epithelial cells massive leukocytes...
Prompt and effective elicitation of protective immunity is highly relevant for cases rapidly deteriorating fatal diseases, such as plague, which caused by
The global increase in multidrug-resistant (MDR) pathogenic bacteria has led to growing interest bacteriophage (“phage”) therapy. Therapeutic phages are usually selected based on their ability infect and lyse target bacteria, using vitro assays. In these assays, phage infection is determined grown standard commercial rich media, while evaluation of the actual therapeutic activity requires presence human blood. present work, we characterized two different Yersinia pestis lytic (ϕA1122 PST)...
Plague pandemics and outbreaks have killed millions of people during the history humankind. The disease, caused by bacteria Yersinia pestis, is currently treated effectively with antibiotics. However, in case multidrug-resistant (MDR) bacteria, alternative treatments are required. Bacteriophage (phage) therapy has shown efficient antibacterial activity various experimental animal models human patients infected different MDR pathogens. Here, we evaluated efficiency фA1122 PST phage therapy,...
The generation of adaptive immunity by vaccination is usually a prolonged process that requires multiple dosing over several months. Hence, vaccines are administered for disease prevention relatively long time prior to possible infection as opposed post-exposure prophylaxis, which typically rapid intervention such antibiotic therapy. emergence pathogens resistant common treatments has prompted the search alternative therapeutic strategies. We previously demonstrated mice with F1 capsular...
Bacterial infection of the lungs triggers a swift innate immune response that involves production cytokines and chemokines promote recruitment cells from bone marrow (BM) into infected tissue limit ability pathogen to replicate. Recent in vivo studies pneumonic plague animal models indicate pulmonary pro-inflammatory airway with Yersinia pestis is substantially delayed comparison other pathogens. Consequently, uncontrolled proliferation observed, followed by dissemination internal organs...
Recent advances in the field of cell therapy have proposed new solutions for tissue repair and regeneration using various delivery approaches. Here we studied ex vivo a novel topical system encapsulated cells hybrid polyethylene glycol-fibrinogen (PEG-Fb) hydrogel microspheres to respiratory tract models. We investigated basic parameters encapsulation, release conditions inflamed damaged lungs bacterial-infected mice. The establishment each step study was essential proof concept....
Pneumonic plague is an infectious disease characterized by rapid and fulminant development of acute pneumonia septicemia that results in death within days exposure. The causative agent pneumonic plague, Yersinia pestis (Y. pestis), a Tier-1 bio-threat agent. Parenteral antibiotic treatment effective when given narrow therapeutic window after symptom onset. However, the non-specific "flu-like" symptoms often lead to delayed diagnosis therapy. In this study, we evaluated inhalational...
Protein translocation across the endoplasmic reticulum is mediated by signal recognition particle (SRP). In this study, SRP pathway in trypanosomatids was down-regulated two approaches: RNA interference (RNAi) silencing of genes encoding proteins Trypanosoma brucei and overexpression dominant-negative mutants 7SL Leptomonas collosoma. The biogenesis both peptide-containing polytopic membrane examined using endogenous green fluorescent protein-fused proteins. RNAi SRP54 or SRP68 T. resulted...
Pneumonic plague, caused by Yersinia pestis, is a rapidly progressing contagious disease. In the plague mouse model, single immunization with EV76 live attenuated Y. pestis strain induced expression of hemopexin and haptoglobin in lung serum, both which are important iron sequestration. Immunization against concomitant lethal respiratory challenge was correlated temporary inhibition disease progression. Combining EV76-immunization second-line antibiotic treatment, individually insufficient,...
We have previously shown that the cell morphogenesis NlpD lipoprotein is essential for virulence of plague bacteria, Yersinia pestis. To elucidate role in Y. pestis pathogenicity, we conducted a whole-genome comparative transcriptome analysis wild-type strain and an nlpD mutant under conditions mimicking early stages infection. The suggested involved three phenomena: (i) Envelope stability/integrity evidenced by compensatory up-regulation Cpx Psp membrane stress-response systems mutant; (ii)...