A5091898632- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Animal Disease Management and Epidemiology
- Full-Duplex Wireless Communications
- Pneumocystis jirovecii pneumonia detection and treatment
- Immunodeficiency and Autoimmune Disorders
- HIV, Drug Use, Sexual Risk
- Biochemical and Molecular Research
- Hepatitis B Virus Studies
- Polyomavirus and related diseases
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- T-cell and Retrovirus Studies
- Sex work and related issues
- Comparative Literary Analysis and Criticism
- Vector-Borne Animal Diseases
- Blood groups and transfusion
- Viral-associated cancers and disorders
- Spanish Culture and Identity
- Vaccine Coverage and Hesitancy
- Influenza Virus Research Studies
- HIV/AIDS oral health manifestations
- Chronic Lymphocytic Leukemia Research
Universidad Independiente
2023
Instituto de Salud Carlos III
1992-2009
Centro Nacional de Microbiologia
2003-2009
Escuela Nacional de Sanidad
2005
The aim of this study was to investigate the susceptibility T20 and dynamics amino acid changes in HR1 HR2 gp41 HIV-1 obtained from plasma, peripheral blood mononuclear cells (PBMC), primary isolates (PI) four highly antiretroviral-experienced patients. These patients received plus an antiretroviral regimen were followed-up over a period 40–72 weeks. In one non-responder patient, N43D substitution detected at 12 weeks treatment, association with value T20-IC50 10 µg/ml (10-fold increase)....
The HIV-1 subtype A epidemic affecting injecting drug users (IDU) in former Soviet Union (FSU) countries started dramatically Odessa, southern Ukraine, 1995, and is caused by a variant of monophyletic origin, often designated IDU-A. We phylogenetically analyzed one near full-length genome two partial sequences three viruses collected St. Petersburg, Russia, heterosexually transmitted 1992–1994. branched basally to the IDU-A clade, together with eight from Odessa 1993, all presumably acquired...
Objective: To determine the introduction of HIV-1 genetic forms and to examine transmission clusters resistance antiretroviral inhibitors among newly diagnosed patients from Basque Country, Spain, during 2004-2007. Methods: A total 261 samples, corresponding 47.5% heterosexuals, 37.9% men who have sex with (MSM), 11.1% intravenous drug users were analyzed in protease reverse transcriptase phylogenetic relationships resistance-associated mutations. Results: Subtype B was detected 220 (84.3%)...
The main objective of this study is to evaluate the prevalence resistance-associated mutations reverse transcriptase (RT) and protease (PR) inhibitors (I) 2 years after introduction antiretroviral treatment in Cuba, analyzing corresponding different HIV-1 genetic forms circulating Cuba. A total 425 plasma samples were collected 2003, 175 (41.2%) subtype B 250 (58.8%) non-B forms, including 56 (22.4 %) subtypes, 112 (44.8%) recombinant (CRFs), 82 (32.8%) unique RFs (URFs). Of these, patients...
Abstract There are few data on drug resistance‐associated mutations in the former Soviet Union since, studies have usually been focused env or gag genes for subtype information. This study examines prevalence and patterns of to reverse transcriptase protease inhibitors (RTI, PRI) 278 HIV‐1‐infected treatment‐naïve subjects from countries Eastern Europe, defines characteristic polymorphisms RT PR sequences HIV‐1 A viruses. Blood samples were collected between 1997 2004. Plasma RNA was used...
The aim of this study was the development a panel constituted by well-defined HIV-1 strains different genetic forms, with particular focus on isolates from acute and recent infections. Fourteen isolates, including four five infections, were expanded in peripheral blood mononuclear cells. SI phenotype, coreceptors use, TCID50/ml determined. V3 net charge calculated. Near full-length genomes amplified RT-nested PCR overlapping segments. Phylogenetic analyses performed neighbor-joining trees...
The objectives of this study are to describe the incidence non-B and recombinant HIV-1 strains in newly diagnosed infections Galicia, northwest Spain, during a 2-year period (May 2000 June 2002), frequency resistance-associated mutations reverse transcriptase (RT) protease (PR) genes, analysing polymorphisms more frequently detected viruses. All HIV-1-infected patients attending nine public hospitals seven main cities Galicia were included study. RT, PR V3 regions from RNA plasma amplified...
The biological characteristics of HIV-1 primary isolates different recombinant forms (RFs) and non-B subtypes from Galicia, Spain, were investigated the relationships between phenotype evolution infection determined. Peripheral blood mononuclear cells (PBMCs) obtained during follow-up 32 patients infected with non-subtype B genetic forms, characterized in partial sequences pol (protease-reverse transcriptase) env V3 region: 12 (37.5%) circulating RFs (CRFs), 9 (28.1%) unique (URFs),...
Abstract Hepatitis B viral serum markers suggestive of infection by hepatitis virus type 2 were found in 354 patients tested the laboratory during a 2.5 year period study. Confirmation HBsAg reactivity neutralization and subtyping analysis determination HBV‐DNA molecular hybridization carried out on selected samples from these patients. Clinical epidemiological data obtained 234 serological follow‐up was done 70 cases. The results confirmatory tests DNA assays indicated that reactivities...
The aim of this study was to characterize the HIV-1 intersubtype recombinant forms generated during follow-up a dual natural infection with subtypes B and G. Near full-length sequences from plasma peripheral blood mononuclear cell (PBMC) compartments were analyzed biological characteristics their derived primary isolates studied. Different mutations detected in V1, V2, V3 but not RNA or PBMC DNA. near sequence first collected subtype G presence subpopulations observed subtype-specific...
The oligonucleotide ligation assay is a genotypic for the detection of resistance-associated mutations to reverse transcriptase and protease inhibitors in human immunodeficiency virus type 1 subtype B. This has been modified developed non-B subtypes recombinant strains evaluated with sequencing, resulting more sensitive than sequencing subtypes.
The presence of resistance-related mutations in 185 serial proviral DNA samples from 108 HIV-infected patients was monitored using the line probe assay (LiPA). proportions wild-type and mutant virus each sample were determined. Subsequent same patient analysed. Resistance detected 58 studied (53.7%), 53 73 (72.6%) treated with antivirals 5 35 (14.2%) untreated. against zidovudine (51), lamivudine (1), (4), zalcitabine (1) didanosine (1). Among resistant virus, 168 related observed: 161 to...
Este articulo presenta las diversas facetas de la vida del connotado escritor Carlos Luis Fallas Sibaja asi como actualidad su pensamiento