This study was done to identify pesticide-biodegrading microorganisms and characterize degradation rates. Bacillus safensis strain FO-36bT, subtilis subsp. inaquosorum KCTC13429T, cereus ATCC14579T were isolated from pesticide-polluted soil in Sudan, separately incubated with each pesticide periodic samples drawn for GC GC-MS. Pesticide biodegradation followed a biphasic model. α β half-lives (days) of chlorpyrifos, malathion, dimethoate B. culture 2.13, 4.76; 2.59, 5.66; 9.5, 11.0,...

Biodegradation of endosulfan (α and β) pendimethalin by Bacillus safensis strain FO-36bT, subtilis subsp. inaquosorum KCTC 13429T cereus ATCC14579T isolated from pesticides-polluted soil was studied in mineral salt medium. Endosulfan were incubated with the three bacterial strains samples drawn at various intervals for GC analysis. Representative subject to GC–MS The loss initial concentrations, 0.663 mM endosulfan), 0.319 (β endosulfan) 1.423 (pendimethalin), monitored used compute...

This study was performed to investigate the residual characteristics, safety assessment, and pre-harvest interval (PHI) of spiromesifen chromafenozide in lettuce (Latuca sativa L.) perilla (Perilla frutescens (L.) Britton) leaves. Samples were harvested periodically, extracted using QuEChERS method, analyzed by LC-MS/MS. Average recoveries its metabolite BSN2060-enol ranged from 80.6 107.9%, with relative standard deviation < 10%. Spiromesifen cromafenozide initial residues dissipated 81.45...

In this study, the residual behavior and safety of cyantraniliprole indoxacarb applied to wild garlic (Allium vineale) were investigated. Samples harvested after treatments 0, 3, 7, 14 days, then prepared extracted following QuEChERS method analyzed by UPLC-MS/MS. The linearity (R2 ≥ 0.99) calibration curves was excellent for both compounds. average recoveries at two spiking concentrations (0.01 0.1 mg/kg) ranged from 94.2% 111.4%. relative standard deviation value below 10%. initial in...

A descriptive cross sectional study was carried out in Khartoum State to assess the knowledge, attitude, and practice of farmers towards pesticide residues on vegetables grown greenhouses. Fourteen from seven localities were selected interviewed. The results revealed that majority (92.9%) between age 25 45 years old, while only one farmer (7.1%) aging more than old. Regarding educational level, showed 20% illiterates, whereas 40% had completed secondary high school, university graduates....

Eggplant is a popular food item in Sudan, however pesticides are heavily used.To investigate the presence of pesticide residues fresh eggplants Khartoum State, Sudan.Eggplant fruit samples from three different regions State (central vegetable market, east Nile farms, and west farms) were analyzed for commonly used pesticides. Pesticide by gas chromatography coupled with mass spectrometry results expressed μg/kg fruit.Out 11 active ingredients analyzed, identified four (imidacloprid,...

Pesticide residue analysis in medicinal herbs is a challenging task because of the matrix effect and its influence on quantitative despite continuous development several new analytical methods instrumentations. In this study, modified QuEChERS method was developed for indoxacarb by using conventional instrument, gas chromatography micro-electron-capture-detector (GC–μECD), comparing it with chromatography–tandem mass spectrometry (GC–MS/MS) liquid (LC–MS/MS). Samples were extracted...

Pesticides have been used for decades to protect agricultural products and increase productivity by controlling crop pests. However, the frequent application of pesticides on crops or soil leads accumulation their residues in environment, which will be subsequently absorbed plants finally translocated edible parts. This study aimed investigate uptake soil-applied dinotefuran three major metabolites lettuce celery from previous season’s applications analyze consumable Dinotefuran was at plant...

The knowledge of eggplant farmers on the proper use pesticides was evaluated in Khartoum State. Questionnaire distributed to North Nile Farms (NN), East (ENF), and West (WNF) solicit information. Interviews questionnaires revealed that there is a limited level awareness among farmers. majority (94%) did not abide by recommended disposal procedures, they only damage bury containers inside farms. (66%) were aware safety period for any given product. excessive production reliance as pest...

Laboratory experiments were conducted to evaluate the efficacy of three types garlic oils; Sudanese, Chinese and Egyptian on eggs cowpea beetle Callosobruchus maculatus.Volatile oils from Sudanese obtained by steam distillation Soxhlet extraction using ethanol correspondingly, whereas oil was bought as a ready-made product.The tested at concentrations 0.01%, 0.1%, 1%, 5% 10%, exposure periods ranging 24-72 hrs.Respective average number laid after lowest concentration were; 5.4, 4.0, 4.1 24...

This study was conducted to investigate the residual behavior and safety assessment of fenazaquin metaflumizone in butterbur. The samples were periodically harvested, extracted using QuEChERS method, determined by LC-MS/MS. linearity matrix-matched calibration curve ≥0.99 for both compounds. average recoveries at two fortification levels (0.01 0.1 mg kg-1) ranged from 86.6 97.2%. relative standard deviation <10%. After 7 days, initial residues butterbur dissipated 79 78%, with respective...

Soil is the final depot of most environmental contaminants, including pesticides. may be contaminated by pesticides as a result direct application or drift during spray activities. contamination with pesticide residues affect quality food crops, animal products, and soil micro-organisms which in turn negatively human health environment.The main objective current study was to determine commonly used rain-fed crops grown Nuba Mountains Cotton Corporation (NMCC) South Kordofan state Western...

The potential of sunlight photolysis in remediation pesticide-polluted soils Sudan is reported. common pollutants endosulfan (alpha- and beta- isomers) pendimethalin were exposed to over glass soil surfaces the periodically drawn samples analyzed by GC-ECD GC-MS. Photo-degradation followed biphasic model. half-lives direct two pesticides ranged between 1.840-6.720 d 2.711 9.760 respectively. respective values for indirect with β-carotene surface 0.846-1.605 benzophenone 1.050-1.626 d. Values...

The study aimed to estimate the level of infestation and percent caused by sorghum chafer (Pachnoda interrupta) (Coleoptera: Scarabaeidae) on sunflower crops in Gedarif State.The also investigates feeding behavior beetle.A field survey was conducted State four localities (University Gedarif, AL Azaza Sogora, Pasonda, Algadambalia).In each locality two (sunflower sorghum) farms were inspected, except at University only no grown.Observations selected made 60 randomly plants (10 footsteps from...

This study developed a suitable analytical method for kasugamycin residues in Achyranthes japonica using LC/MS/MS equipped with an amide column polar substances. Extraction and cleanup processes were done at pH 4.5–5. Purification efficiency was assessed confirmed step by selecting silica, hydrophilic-lipophilic balance (HLB), strong cation exchange (SCX), double (HLB SCX) SPE cartridges. The results indicated that silica cartridge exhibited overloading tendency, while HLB had low cleaning...

Background: Angelica dahurica Radix and Osterici radix, are perennial herbs belonging to the family Umbelliferae, which were attacked by red spiders, thus fenpropathrin was registered. Fenpropathrin is important in improving productivity quality of medicinal due timely pest control. However, residue may likely remain after harvest, therefore, analysis ensure pesticides safety management. Objective: This study aimed establish method for residues A. O. radix using GLC-ECD GC-MS/MS. Methods:...

Field assessment of integrated management BT cotton pests was carried out in two consecutive seasons; 2016/2017 and 2017/2018 the experimental research farm Kassala University, Sudan. Four insecticides (imidacloprid, abamectin, profenofos, lufenuron) from different chemical groups with a mode action three levels (43.81 kgha-1, 87.62 131.43 kgha-1) soil-applied nitrogen were tested IPM package. Insecticides applied at recommended doses. A total 7 weekly counts prevailing natural enemies...