A5103132402- Pregnancy and preeclampsia studies
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Pluripotent Stem Cells Research
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Preterm Birth and Chorioamnionitis
- Single-cell and spatial transcriptomics
- Infant Nutrition and Health
- Mesenchymal stem cell research
- Gynecological conditions and treatments
- Birth, Development, and Health
- Endometriosis Research and Treatment
- Epigenetics and DNA Methylation
University of California, San Diego
2022-2024
Sanford Consortium for Regenerative Medicine
2022-2024
Northwestern University
2020-2021
Trophoblast stem cells (TSCs) have recently been derived from human embryos and early-first-trimester placenta; however, aside ethical challenges, the unknown disease potential of these limits their scientific utility. We previously established a bone morphogetic protein 4 (BMP4)-based two-step protocol for differentiation primed pluripotent (hPSCs) into functional trophoblasts; those trophoblasts could not be maintained in self-renewing TSC-like state. Here, we use first step this protocol,...
Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the endometrium, such as spontaneous decidualization, no existing cell cultures recapitulate cyclic interactions between stromal epithelial compartments necessary for decidualization implantation. Here we report a protocol differentiating pluripotent stem cells into fibroblasts (PSC-ESFs) that are highly pure able to decidualize....
Functional annotations of a placental cell genome aid in identifying genes associated with preterm birth.
Preeclampsia (PE) is a hypertensive pregnancy disorder with increased risk of maternal and fetal morbidity mortality. Abnormal extravillous trophoblast (EVT) development function considered to be the underlying cause PE, but has not been previously modeled
Cytotrophoblast (CTB) of the early gestation human placenta are bipotent progenitor epithelial cells, which can differentiate into invasive extravillous trophoblast (EVT) and multinucleated syncytiotrophoblast (STB). Trophoblast stem cells (TSC), derived from first trimester placentae, have also been shown to be bipotential. In this study, we set out probe transcriptional diversity CTB compare TSC various subgroups CTB. We performed single-cell RNA sequencing on six normal four (6-8 weeks)...
Abstract We previously established a trophoblast differentiation protocol from primed human pluripotent stem cells (PSC). To induce this lineage, we use combination of Bone Morphogenetic Protein‐4 (BMP4) and the WNT inhibitor IWP2. This has enabled us to obtain pure population trophectoderm (TE)‐like that could subsequently be terminally differentiated into syncytiotrophoblasts (STB) extravillous trophoblasts (EVT). However, resulting TE‐like only variable mixture STB EVT, with bias toward...
Abstract While a genetic component of preterm birth (PTB) has long been recognized and recently mapped by genome-wide association studies (GWAS), the molecular determinants underlying PTB remain elusive. This stems in part from an incomplete availability comprehensive functional genomic annotations human cell types relevant to pregnancy PTB. Here, we generated extensive transcriptional chromatin cultured primary decidua-derived mesenchymal stromal/stem cells (MSCs) vitro differentiated...