A5051658172- Retinal Development and Disorders
- Zebrafish Biomedical Research Applications
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Retinal Imaging and Analysis
- Photoacoustic and Ultrasonic Imaging
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Optical measurement and interference techniques
- Workplace Violence and Bullying
- Gun Ownership and Violence Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Telemedicine and Telehealth Implementation
- Surface Roughness and Optical Measurements
- Suicide and Self-Harm Studies
- Sarcoidosis and Beryllium Toxicity Research
- Optical Polarization and Ellipsometry
- Ophthalmology and Visual Impairment Studies
- Vasculitis and related conditions
- Congenital heart defects research
- Infrared Target Detection Methodologies
- melanin and skin pigmentation
- Patient-Provider Communication in Healthcare
- Eosinophilic Disorders and Syndromes
Washington University in St. Louis
2022-2024
Barnes-Jewish Hospital
2023
Medical College of Wisconsin
2016-2022
Quantification of the human rod and cone photoreceptor mosaic in adaptive optics scanning light ophthalmoscope (AOSLO) images is useful for study various retinal pathologies. Subjective time-consuming manual grading has remained gold standard evaluating these images, with no well validated automatic methods detecting individual rods having been developed. We present a novel deep learning based method, called CNN (RAC-CNN), classifying cones multimodal AOSLO images. test our method on from...
To demonstrate and validate that photothermal optical coherence tomography (PT-OCT) can image melanin in the retinal pigment epithelium (RPE) observe light-driven melanosome translocation zebrafish retina.A commercial spectral domain OCT system was modified to perform both PT-OCT. Four adult tyrosinase-mosaic with varying levels of expression across their retinas were imaged, PT-OCT signal for pigmented nonpigmented regions compared. Wild-type dark-adapted (n = 11 fish) light-adapted 10 also...
We assessed the effect of melanin on appearance hyperreflective outer retinal bands in optical coherence tomography (OCT) images.A total 23 normal subjects and 51 patients with albinism were imaged using Bioptigen high-resolution spectral-domain OCT. In addition, three wild type, albino (slc45a2b4/b4 ), eight tyrosinase mosaic zebrafish hand-held Envisu R2200 To identify pigmented versus nonpigmented regions zebrafish, en face summed volume projections pigment epithelium (RPE) created from...
Abstract Zebrafish ( Danio rerio ) provide many advantages as a model organism for studying ocular disease and development, there is great interest in the ability to non-invasively assess their photoreceptor mosaic. Despite recent applications of scanning light ophthalmoscopy, fundus photography, gonioscopy vivo imaging adult zebrafish eye, current techniques either lack accurate scaling information (limiting quantitative analyses) or require euthanizing fish (precluding longitudinal...
To examine repeatability and reproducibility of ellipsoid zone (EZ) width measurements in patients with retinitis pigmentosa (RP) using a longitudinal reflectivity profile (LRP) analysis.We examined Bioptigen optical coherence tomography (OCT) scans from 48 subjects RP or Usher syndrome. Nominal scan lengths were 6, 7, 10 mm, the lateral scale each was calculated axial length measurements. LRPs generated OCT line scans, peak corresponding to EZ manually identified ImageJ. The locations at...
To evaluate different methods of studying cone photoreceptor structure in wild-type (WT) and transgenic pigs carrying the human rhodopsin P23H mutant gene (TgP23H).For vivo imaging, were anesthetized with tiletamine-zolazepam isoflurane given lidocaine-bupivacaine retrobulbar injections. Stay sutures a custom head mount used to hold steer for adaptive optics scanning light ophthalmoscopy (AOSLO). Six WT TgP23H littermates imaged at postnatal day 30 (P30), P90, P180 AOSLO optical coherence...
Purpose: To observe and characterize cone degeneration regeneration in a selective metronidazole-mediated ablation model of ultraviolet-sensitive (UV) cones zebrafish using vivo optical coherence tomography (OCT) imaging. Methods: Twenty-six sws1:nfsB-mCherry;sws2:eGFP were imaged with OCT, treated metronidazole to selectively kill UV cones, at 1, 3, 7, 14, 28, or 56 days after ablation. Regions 200 × µm cropped from volume OCT scans count individual before Fish eyes fixed,...
This qualitative improvement study examines the change in electronic health record messaging from patients to mental professionals before vs after COVID-19 pandemic.
Leber congenital amaurosis (LCA) is a genetic disease associated with blindness that caused by mutations in retinal pigment epithelium (RPE65), an enzyme involved the visual cycle. When light hits photosensitive pigments retina, it conformationally changes 11‐cis‐retinol (a form of vitamin A) to 11‐trans‐retinol. This conversion allows for series chemical reactions and formation electrical signals communicate brain. RPE65 essential cycle because 11‐trans‐retinol back into...
Stroke has become increasingly common with the rise in hyperlipidaemia, hypertension and other metabolic disorders. In this case, a 69-year-old man several weeks of non-specific symptoms no history disorders presents unilateral weakness supraventricular tachycardia. Kidney biopsy confirmed diagnosis eosinophilic granulomatosis polyangiitis. He responded well to rituximab infusions.