A5071386899- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Chronic Kidney Disease and Diabetes
- Renal cell carcinoma treatment
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- Single-cell and spatial transcriptomics
- Calcium signaling and nucleotide metabolism
Radboud University Nijmegen
2008-2022
Radboud University Medical Center
2008-2022
Radboud Institute for Molecular Life Sciences
2021-2022
Nephrotic syndrome (NS) is characterized by severe proteinuria as a consequence of kidney glomerular injury due to podocyte damage. In vitro models mimicking in vivo characteristics are prerequisite resolve NS pathogenesis. The detailed characterization organoid podocytes resulting from hybrid culture protocol showed population that resembles adult and was superior compared with 2D counterparts, based on single-cell RNA sequencing, super-resolution imaging electron microscopy. this study,...
Abstract Scattered tubular cells (STCs) are a phenotypically distinct cell population in the proximal tubule that increase number after acute kidney injury. We aimed to characterize human STC population. Three‐dimensional tissue analysis revealed STCs preferentially located within inner bends of and barely present young (<2 years), their increases with age. Increased numbers were associated injury (kidney molecule 1) interstitial fibrosis (alpha smooth muscle actin). Isolated CD13 + CD24...
ADP-ribosylation factor (Arf) proteins are small guanosine triphosphatases (GTPases) that act as major regulators of intracellular vesicular trafficking and secretory organelle pathway integrity. Like all monomeric GTPases, Arf cycle between a GDP-bound GTP-bound state, this cycling is catalysed by guanine nucleotide exchange factors (GEFs) GTPase-activating proteins. While the class I Arfs, especially Arf1, have been studied extensively, little known yet about function regulation II Arf4...
ABSTRACT In the glomerulus, Bowman's space is formed by a continuum of glomerular epithelial cells. focal segmental glomerulosclerosis (FSGS), glomeruli show scarring, result activated parietal cells (PECs) invading tuft. The scars interrupt continuum. However, non-sclerotic segments seem to be preserved even in with advanced lesions. We studied histology pattern Munich Wistar Frömter rats, model for secondary FSGS. Our results showed that matrix layers lined PECs cover sclerotic These...
Abstract Nephrotic syndrome (NS) is characterized by severe proteinuria as a consequence of kidney glomerular injury due to podocyte damage. In vitro models mimicking in vivo characteristics are prerequisite resolve NS pathogenesis. Here, we report human induced pluripotent stem cell derived organoids containing population that heads towards adult podocytes and were superior compared 2D counterparts, based on scRNA sequencing, super-resolution imaging electron microscopy. this study, these...
Parietal epithelial cells (PECs) are in the kidney, surrounding Bowman's space. When activated, PECs increase cell volume, proliferate, migrate to glomerular tuft and excrete extracellular matrix. Activated crucially involved formation of sclerotic lesions, seen focal segmental glomerulosclerosis (FSGS). In FSGS, a number glomeruli show lesions. Further disease progression will lead increasing gradual destruction affected glomeruli. Although involvement FSGS has been acknowledged, little is...