A5074798362- Acute Ischemic Stroke Management
- CRISPR and Genetic Engineering
- Scientific Computing and Data Management
- Electron Spin Resonance Studies
- Pluripotent Stem Cells Research
- Machine Learning in Healthcare
- Stroke Rehabilitation and Recovery
- Artificial Intelligence in Healthcare
- Cerebrovascular and Carotid Artery Diseases
- Photosynthetic Processes and Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Biomedical Text Mining and Ontologies
- Photoreceptor and optogenetics research
- Research Data Management Practices
- Hemoglobin structure and function
- 3D Printing in Biomedical Research
- Photochemistry and Electron Transfer Studies
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Semantic Web and Ontologies
- Ethics in Clinical Research
- Photochromic and Fluorescence Chemistry
- Electrochemical sensors and biosensors
- Advanced Database Systems and Queries
National Institute of Neurological Disorders and Stroke
2013-2025
National Institutes of Health
2013-2025
China Medical University Hospital
2024
China Medical University
2024
Tri-Service General Hospital
2024
Office of Extramural Research
2008-2024
Office of the Director
2024
National Defense Medical Center
2024
Johns Hopkins University
2024
Johns Hopkins Medicine
2024
The oxidation of the fluorescent dye 2′,7′-dichlorofluorescein (DCF) by horseradish peroxidase was investigated optical absorption, electron spin resonance (ESR), and oxygen consumption measurements. Spectrophotometric measurements showed that DCF could be oxidized either peroxidase-compound I or -compound II with obligate generation phenoxyl radical (DCF⋅). This one-electron confirmed ESR spin-trapping experiments. DCF⋅ oxidizes GSH, generating glutathione thiyl (GS⋅), which detected...
A stringent control of homeostasis is critical for functional maintenance and survival neurons. In the mammalian retina, basic motif leucine zipper transcription factor NRL determines rod versus cone photoreceptor cell fate activates expression many rod-specific genes. Here, we report an integrated analysis NRL-centered gene regulatory network by coupling chromatin immunoprecipitation followed high-throughput sequencing (ChIP–Seq) data from Illumina ABI platforms with global profiling in...
Intratracheal instillation of lipopolysaccharide (LPS) activates alveolar macrophages and infiltration neutrophils, causing lung injury/acute respiratory distress syndrome. Free radicals are a special focus as the final causative molecules in pathogenesis injury caused by LPS. Although vitro investigation has demonstrated radical generation after exposure cells to LPS, vivo evidence is lacking. Using electron spin resonance (ESR) trap alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN), we...
A large superfamily of transmembrane receptors control cellular responses to diverse extracellular signals by catalyzing activation specific types heterotrimeric GTP-binding proteins. How these recognize and promote nucleotide exchange on G protein α subunits initiate signal amplification is unknown. The three-dimensional structure the transducin (Gt) subunit C-terminal undecapeptide Gtα(340–350) IKENLKDCGLF was determined transferred nuclear Overhauser effect spectroscopy while it bound...
Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to embryonic (hESCs) yet picture remains unclear. To address this, we derived a population neural precursor (NPCs) from H1 (WA01) hESC line generated isogenic iPSC lines by reprogramming. The gene expression methylation profile three were parental intermediate NPC population. We found no probe with that differed significantly between samples under undifferentiated or differentiated...
Abstract Automated coronary angiography assessment requires precise vessel segmentation, a task complicated by uneven contrast filling and background noise. Our research introduces an ensemble U-Net model, SE-RegUNet, designed to accurately segment vessels using 100 labeled angiographies from angiographic images. SE-RegUNet incorporates RegNet encoders squeeze-and-excitation blocks enhance feature extraction. A dual-phase image preprocessing strategy further improves the model's performance,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of CuB in the Binuclear Heme-Copper Center Cytochrome aa3-Type Quinol Oxidase from Bacillus subtilis: An ENDOR and EXAFS StudyYang C. Fann, Ishak Ahmed, Ninian J. Blackburn, John S. Boswell, Marina L. Verkhovskaya, Brian M. Hoffman, Marten WikstromCite this: Biochemistry 1995, 34, 32, 10245–10255Publication Date (Print):August 15, 1995Publication History Published online1 May 2002Published inissue 15 August...
Acquisition of virulence factors and antibiotic resistance by many clinically important bacteria can be traced to horizontal gene transfer (HGT) between related or evolutionarily distant microflora. Comparative genomic analysis has become an tool for identifying HGT DNA in emerging pathogens. We have adapted the multi-genome alignment EvoPrinter facilitate discovery sequences within bacterial genomes their mobile genetic elements.EvoPrinter 13 different Staphylococcus aureus revealed that...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetection of two histidyl ligands to CuA cytochrome oxidase by 35-GHz ENDOR. 14,15N and 63,65Cu ENDOR studies the site in bovine heart aa3 cytochromes caa3 ba3 from Thermus thermophilusRyszard J. Gurbiel, Yang Cheng Fann, Kristene K. Surerus, Melanie M. Werst, Siegfried Musser, Peter E. Doan, Sunney I. Chan, James A. Fee, Brian HoffmanCite this: Am. Chem. Soc. 1993, 115, 23, 10888–10894Publication Date (Print):November 1, 1993Publication History...
The reaction of cytochrome c and H(2)O(2) is known to form a protein-centred radical that can be detected with the spin trap 2-methyl-2-nitrosopropane (MNP). To characterize MNP/tyrosyl adduct structure had previously been determined incorrectly [Barr, Gunther, Deterding, Tomer Mason (1996) J. Biol. Chem. 271, 15498-15503], we eliminated unreasonable models by ESR studies series (13)C-labelled tyrosines, photochemically synthesized an authentic has its trapping site on C-3 position tyrosine...
The reaction of cytochrome c and H2O2 is known to form a protein-centred radical that can be detected with the spin trap 2-methyl-2-nitrosopropane (MNP). To characterize MNP/tyrosyl adduct structure had previously been determined incorrectly [Barr, Gunther, Deterding, Tomer Mason (1996) J. Biol. Chem. 271, 15498–15503], we eliminated unreasonable models by ESR studies series 13C-labelled tyrosines, photochemically synthesized an authentic has its trapping site on C-3 position tyrosine phenyl...
Abstract Background Multi-genome comparative analysis has yielded important insights into the molecular details of gene regulation. We have developed EvoPrinter , a web-accessed genomics tool that provides single uninterrupted view conserved sequences as they appear in species interest. An EvoPrint reveals with near base-pair resolution those are essential for function. Results describe here EvoPrinterHD 2 nd -generation automatically generates from input sequence an enhanced conservation...
Multimorbidity is common in patients who experience stroke. Less known about the effect of specific multimorbidity patterns on long-term disability with Furthermore, given increased poststroke frequently seen female vs male patients, it unknown whether has a similar association both sexes. We assessed clusters were associated greater burden overall and by sex.
ABSTRACT One of the most important properties human embryonic stem cells (hESCs) is related to their primed and naïve pluripotent states. Our previous meta-analysis indicates existence heterogeneous states derived from diverse protocols. In this study, we have characterized a commercial medium (RSeT)-based state under various growth conditions. Notably, RSeT hESCs can circumvent hypoxic conditions as required by hESCs, in which some (e.g., H1 cells) exhibit much lower single cell plating...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTENDOR Determination of Heme Ligation in Chloroperoxidase and Comparison with Cytochrome P-450CamYang-Cheng Fann, Nancy C. Gerber, Pawel A. Osmulski, Lowell P. Hager, Stephen G. Sligar, Brian M. HoffmanCite this: J. Am. Chem. Soc. 1994, 116, 13, 5989–5990Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June...
Several variants of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are emerging all over world. Variant surveillance from genome sequencing has become crucial to determine if mutations in these rendering virus more infectious, potent, or resistant existing vaccines and therapeutics. Meanwhile, analyzing many raw data repeatedly with currently available code-based bioinformatics tools is tremendously challenging be implemented this unprecedented pandemic time due fact...