A5100785944- Helminth infection and control
- Coccidia and coccidiosis research
- Protein Hydrolysis and Bioactive Peptides
- Aquaculture disease management and microbiota
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Invertebrate Immune Response Mechanisms
- Parasites and Host Interactions
- interferon and immune responses
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Physiological and biochemical adaptations
- Reproductive biology and impacts on aquatic species
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Sperm and Testicular Function
- Coral and Marine Ecosystems Studies
- Chemokine receptors and signaling
- Control Systems and Identification
- Microbial Community Ecology and Physiology
- Mathematical Control Systems and Analysis
- Drug Transport and Resistance Mechanisms
- Quality and Safety in Healthcare
- Parasitic Diseases Research and Treatment
- Heat shock proteins research
BGI Research
2023-2024
Botswana Geoscience Institute
2024
Institute of Deep-Sea Science and Engineering
2023-2024
Nanjing Agricultural University
2020-2023
Tianjin Medical University
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Chinese Academy of Fishery Sciences
2006-2020
Qingdao National Laboratory for Marine Science and Technology
2016-2019
Institute of Crop Sciences
2017
Chinese Academy of Agricultural Sciences
2017
Songlin Chen and colleagues sequenced the whole genomes of a male (ZZ) female (ZW) Chinese half-smooth tongue sole, Cynoglossus semilaevis. Their analysis provides insights into structure evolution sex chromosomes adaptation to benthic lifestyle this flatfish. Genetic determination by W Z has developed independently in different groups organisms. To better understand plasticity sex-determination mechanisms, we sole (Cynoglossus semilaevis). In addition lifestyle, find that these fish are...
Abstract Chinese tongue sole is a marine fish with ZW sex determination. Genome sequencing suggested that the Z-linked dmrt1 putative male determination gene, but direct genetic evidence still lacking. Here we show TALEN of efficiently induced mutations this gene. The ZZ mutant developed ovary-like testis, and spermatogenesis was disrupted. female-related genes foxl2 cyp19a1a were significantly increased in gonad mutant. Conversely, male-related Sox9a Amh decreased. deficient grew much...
Abstract Gsdf is a key gene for testicular differentiation in teleost. However, little known about the function of Chinese tongue sole ( Cynoglossus semilaevis ). In this study, we obtained full-length CS - ), and functional characterization revealed its potential participation during germ cell testes. transcription was predominantly detected gonads, while levels testes were significantly higher than those ovaries. During different developmental stages male mRNA level upregulated at 86 dph,...
A total of 10 specimens Alcyonacea corals were collected at depths ranging from 905 m to 1 633 by the manned submersible Shenhai Yongshi during two cruises in South China Sea (SCS). Based on mitochondrial genomic characteristics, morphological examination, and sclerite scanning electron microscopy, samples categorized into four suborders (Calcaxonia, Holaxonia, Scleraxonia, Stolonifera), identified as 9 possible new cold-water coral species. Assessments GC-skew dissimilarity, phylogenetic...
<title>Abstract</title> Background To investigate the relationship between emotional dysregulation, as measured by go/nogo task, and multidimensional profile of borderline personality disorder (BPD). Methods Eighty-two participants (32 BPD, 50 controls) completed Personality Assessment Inventory (PAI), Patient Health Questionnaire (PHQ-9), Generalized Anxiety Disorder (GAD-7) scale. EEG was recorded during an Go/Nogo task. Event-related potential (ERP) components were analyzed for...
Pure lines derived from multiple parents are becoming more important because of the increased genetic diversity, possibility to conduct replicated phenotyping trials in environments and potentially high mapping resolution quantitative trait loci (QTL). In this study, we proposed a new method for QTL detection pure-line populations four-way crosses, which is able control background variation through two-stage strategy. First, orthogonal variables were created each marker used an inclusive...
Testis-specific protein kinase 1 (Tesk1) is a serine/threonine with unique structural features. In the present study, we cloned and characterized tesk1 gene of tongue sole, Cynoglossus semilaevis. The full-length cDNA consists 1,672 nucleotides, encoding 331 amino acid polypeptide characteristic structure composed an N-terminal domain C-terminal proline-rich domain. genomic sequence contains eight exons seven introns. Real-time quantitative PCR revealed that mRNA expressed predominantly in...
Th9 cells have been shown to play crucial roles in anti-parasite immunity, pathogenic microbe infection, and allergy. Previous studies demonstrated that Haemonchus contortus excretory secretory proteins (HcESPs) induce the proliferation of alter transcriptional level IL-9 as well its related pathways immune response after infection. However, exact molecule(s) HcESPs inducing is not yet known. In this study, flow cytometry, co-immunoprecipitation (Co-IP) shotgun liquid chromatography...
β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays role cell adhesion, embryogenesis and sex determination. However, little known about its function teleosts. We cloned characterized full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides encodes protein with 782 amino acids. Although transcribed gonads both sexes, level significantly higher ovaries...
Haemonchus contortus Y75B8A.8 (Hc8) derived from H. excretory-secretory (ES) products was identified as a functional inhibitor of goat interleukin 2 (IL-2). It may act vaccine candidate for the development therapeutic strategies against infection. In this research, recombinant Hc8 (rHc8) and anti-rHc8 polyclonal antibodies were employed to evaluate protective capacities antigen infections via active passive immunization trials, respectively. both local crossbred female goats aged 9-12 months...
Trehalose-6-phosphate phosphatase (TPP6) is a key enzyme in the trehalose biosynthesis pathway. The accumulation of TPP6 inside body harmful to pathogen, but almost nothing currently known about function from Haemonchus contortus (CRE-GOB-1). H. CRE-GOB-1 (HcGOB) gene was cloned and recombinant protein GOB (rHcGOB) expressed; transcription HcGOB at different developmental stages then studied. spatial expression pattern adult female male worms determined by both quantitative real-time PCR...
Aquaporin 1 (AQP1) is a member of the transmembrane water channel family proteins with special structural features, and two AQP1 paralogous genes (aqp1aa aqp1ab) are reported in teleosts. In present study, aqp1aa gene half-smooth tongue sole (Cynoglossus semilaevis) was cloned characterized. The full-length cDNA 1411 bp 786 open reading frame encoding 261-amino acid putative protein characteristic structure consisting 6 membrane-spanning α-helical domains highly conserved...
Trehalose phosphate synthase (TPS), a key enzyme in trehalose synthesis, is not present mammals but critical to the viability of wide range lower organisms. However, almost nothing known about function Hc-TPS (GT1-TPS structural domain protein from Haemonchus contortus). In this study, gene was cloned and recombinant (rHc-TPS) expressed purified. The quantitative real-time PCR (qPCR) results showed that transcribed at different stages H. contortus, with higher levels transcription molting...
The utility of the mitochondrial genomes (mitogenomes) in analyzing evolutionary history animals has been proven. Five deep-sea corals (
Sediment plays a pivotal role in deep-sea ecosystems by providing habitats for diverse range of microorganisms and facilitates the cycling processes carbon, sulfur nitrogen. Beyond normal seafloor (NS), distinctive geographical features such as cold seeps (CS) hydrothermal vent (HV) are recognized life oases harboring highly microbial communities. A global atlas can reveal notable association between geological colonization. However, comprehensive understanding systematic comparison...