A5021704605- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Fungal and yeast genetics research
- Cancer-related molecular mechanisms research
- Gene Regulatory Network Analysis
- Advanced biosensing and bioanalysis techniques
Inserm
2018-2023
Institut de génétique et de biologie moléculaire et cellulaire
2014-2023
Université de Strasbourg
2014-2023
Centre National de la Recherche Scientifique
2018-2023
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2014
Fred Hutch Cancer Center
2014
Abstract Cells dedicate significant energy to build proteins often organized in multiprotein assemblies with tightly regulated stoichiometries. As genes encoding subunits assembling a multisubunit complex are dispersed the genome of eukaryotes, it is unclear how these protein complexes assemble. Here, we show that mammalian nuclear transcription (TFIID, TREX-2 and SAGA) composed large number subunits, but lacking precise architectural details built co-translationally. We demonstrate...
Abstract Large heteromeric multiprotein complexes play pivotal roles at every step of gene expression in eukaryotic cells. Among them, the 20-subunit basal transcription factor TFIID nucleates RNA polymerase II preinitiation complex promoters. Here, by combining systematic RNA-immunoprecipitation (RIP) experiments, single-molecule imaging, proteomics and structure–function analyses, we show that human biogenesis occurs co-translationally. We discovered all protein heterodimerization steps...
Most RNA polymerase (Pol) II promoters lack a TATA element, yet nearly all Pol transcription requires binding protein (TBP). While the TBP-TATA interaction is critical for at TATA-containing promoters, it has been unclear whether TBP sequence-specific DNA contacts are required TATA-less genes. Transcription factor IID (TFIID), TBP-containing coactivator that functions most genes, recognizes short promoter elements in metazoans, but analogous have not identified Saccharomyces cerevisiae. We...
Large heteromeric multiprotein complexes play pivotal roles at every step of gene expression in eukaryotic cells. Among them, the 20-subunit basal transcription factor TFIID nucleates RNA polymerase II preinitiation complex promoters. Here, by combining systematic RNA-immunoprecipitation (RIP) experiments, single-molecule imaging, proteomics and structure-function analyses, we show that biogenesis occurs co-translationally. We discovered all protein heterodimerization steps happen during...
A bstract Cells dedicate significant energy to build proteins often organized in multiprotein assemblies with tightly regulated stoichiometries. As genes encoding assembling the same multisubunit complexes are dispersed genome of eukaryotes, it is unclear how assemble. We show that mammalian nuclear transcription (TFIID, TREX-2 and SAGA) composed a large number subunits but lacking precise architectural details built co-translationally. demonstrate dimerization domains their positions...