A5032682113- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- interferon and immune responses
- Cell death mechanisms and regulation
- Cytokine Signaling Pathways and Interactions
- Calcium signaling and nucleotide metabolism
- Plant Pathogenic Bacteria Studies
- Inflammasome and immune disorders
- Bacterial Genetics and Biotechnology
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Plant Parasitism and Resistance
- IL-33, ST2, and ILC Pathways
- Immunodeficiency and Autoimmune Disorders
- Rabies epidemiology and control
- Plant pathogens and resistance mechanisms
- T-cell and B-cell Immunology
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Cell Image Analysis Techniques
- BRCA gene mutations in cancer
- Plant Molecular Biology Research
- Pneumocystis jirovecii pneumonia detection and treatment
Ghent University
2016-2025
VIB-UGent Center for Inflammation Research
2016-2025
Vlaams Instituut voor Biotechnologie
2008-2018
Ghent University Hospital
2017
Swedish University of Agricultural Sciences
2003-2007
Summary Pathogenesis‐related protein 2 ( PR 2) is known to play a major role in plant defense and general stress responses. Resistance against the fungal pathogen Leptosphaeria maculans Arabidopsis requires abscisic acid ABA ), which promotes deposition of callose, β‐1,3‐glucan polymer. Here, we examined callose relation treatment challenge with L. Pseudomonas syringae . Characterization 2‐overexpressing plants knockout line indicated that negatively affects deposition. Recombinant purified...
Autosomal dominant hyper-IgE syndrome (AD-HIES) is typically caused by dominant-negative (DN) STAT3 mutations. Patients suffer from cold staphylococcal lesions and mucocutaneous candidiasis, severe allergy, skeletal abnormalities. We report 12 patients 8 unrelated kindreds with AD-HIES due to DN IL6ST identified seven different truncating mutations, one of which was recurrent. The mutant alleles encode GP130 receptors bearing the transmembrane domain but lacking both recycling motif all four...
Ataxia telangiectasia and Rad3-related (ATR) kinase its interacting protein ATRIP orchestrate the replication stress response. Homozygous splice variants in gene, resulting deficiency, were identified two patients of independent ancestry with microcephaly, primordial dwarfism, recurrent infections. The c.829+5G>T patient exhibited lymphopenia, poor vaccine responses, autoimmune features hemolytic anemia, neutropenia. Immunophenotyping revealed reduced CD16+/CD56dim NK cells absent...
The responses of Arabidopsis accessions and characterized genotypes were used to explore components in the early defense soilborne fungus Verticillium longisporum. V. longisporum susceptibility was found be a complex trait, which different disease phenotypes, such as stunting, altered flowering time, weight loss, chlorosis perceived differently across genotypes. A Bay-0 × Shahdara recombinant inbred line population identify two loci on chromosomes 2 3 origin that caused enhanced after...
Here, we describe the rapid cloning of a plant gene, Leptosphaeria maculans 3 (RLM3(Col)), which encodes putative Toll interleukin-1 receptor-nucleotide binding (TIR-NB) class protein, is involved in defence against fungal pathogen L. and three other necrotrophic fungi. We have, through microarray-based case control bulk segregant comparisons transcriptomes pools Col-0 x An-1 progeny, identified absence locus that causes susceptibility An-1. The significance this on chromosome 4 for...
The paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is crucial for lymphocyte activation through signaling to the transcription factor NF-κB. Besides functioning as a scaffold protein, MALT1 also acts cysteine protease that specifically cleaves number of substrates and contributes specific T cell receptor-induced gene expression. Recently, small molecule inhibitors proteolytic activity were identified shown have promising anticancer properties in...
Abstract Hyperinflammatory syndromes are life-threatening disorders caused by overzealous immune cell activation and cytokine release, often resulting from defects in negative feedback mechanisms. In the quintessential hyperinflammatory syndrome familial hemophagocytic lymphohistiocytosis (HLH), inborn errors of cytotoxicity result effector accumulation, dysregulation and, if untreated, tissue damage death. Here, we describe a human case with homozygous nonsense R688* RC3H1 mutation...
Summary In a cross between the two resistant accessions Col‐0 and L er ‐0, 15:1 segregation was found in F 2 , suggesting presence of unlinked resistance loci to Leptosphaeria maculans. One hundred Col‐4 × 50 ‐2 Cvi‐1 recombinant inbred lines, seven susceptible ‐0 Ws‐0 progenies were examined identify loci. Resistance Col‐4, ( RLM1 ) mapped marker m305 on chromosome 1. mapping populations located RLM2 same arm 4. A tight physical location established through near‐isogenic lines. This region...
Summary Out of 168 Arabidopsis accessions screened with isolates Leptosphaeria maculans , one (An‐1) showed clear disease symptoms. In order to identify additional components involved in containment L. a screen for ‐susceptible ( lms ) mutants was performed. Eleven were isolated, which displayed differential susceptibility responses maculans. lms1 crossed Columbia (Col‐0) and Ws‐0, mapping data both populations the highest linkage region on chromosome 2. Reduced levels PR‐1 PDF1 .2...
Abscisic acid (ABA) is a defense hormone with influence on callose-dependent and -independent resistance against Leptosphaeria maculans acting in the RLM1 Col pathway. ABA-deficient -insensitive mutants Ler-0 background (aba1-3 abi1-1) displayed susceptibility to L. maculans, along significantly decreased level of callose depositions, whereas abi2-1 abi3-1 remained resistant, together abi5-1 mutant Ws-0 background. Suppressor abi1-1 confirmed that maculans-susceptible response was due...
Background: Inherited CARD9 deficiency constitutes a primary immunodeficiency predisposing uniquely to chronic and invasive fungal infections. Certain mutations are shown negatively impact protein expression and/or NF-κB activation, but the underlying biochemical mechanism remains be fully understood. Objectives: To investigate possible founder origin of known R70W mutation in five families Turkish origin. explore by CARD9. Methods: We performed haplotype analysis using microsatellite...
Herpes simplex virus 1 (HSV-1) infects several billion people worldwide and can cause life-threatening herpes encephalitis (HSE) in some patients. Monogenic defects components of the type I interferon system have been identified patients with HSE, emphasizing role inborn errors immunity underlying HSE pathogenesis. Here, we identify compound heterozygous loss-of-function mutations gene GTF3A encoding for transcription factor IIIA (TFIIIA), a component RNA polymerase III complex, patient...