A5034733517- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Respiratory viral infections research
- Reproductive tract infections research
- Biosensors and Analytical Detection
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- Parasitic Infections and Diagnostics
- Virology and Viral Diseases
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- interferon and immune responses
- Macrophage Migration Inhibitory Factor
- Viral-associated cancers and disorders
- Environmental Quality and Pollution
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Toxoplasma gondii Research Studies
- Phagocytosis and Immune Regulation
- Phytoestrogen effects and research
Binzhou Medical University
2012-2024
University of Florida
2021
Beltsville Agricultural Research Center
2014-2018
Agricultural Research Service
2012-2018
United States Department of Agriculture
2012
Cardiovascular Systems (United States)
2007
Search
2007
Thomson Reuters (United States)
2007
Henan Normal University
1996-1997
Academia Sinica
1987
Abstract Host proteins interacting with pathogens are receiving more attention as potential therapeutic targets in molecular medicine. Streptococcus suis serotype 2 (SS2) is an important cause of meningitis both humans and pigs worldwide. SS2 Enolase (Eno) has previously been identified a virulence factor role altering blood brain barrier (BBB) integrity, but the host cell membrane receptor Eno The mechanism(s) involved unclear. This study that binds to 40S ribosomal protein SA (RPSA) on...
The polymorphic membrane protein D (PmpD) is a highly conserved outer which plays an important role in pathogenesis during Chlamydia psittaci infection. In this study, we evaluated the ability of N-terminus PmpD (PmpD-N) to modulate functions chicken macrophages and signaling pathway(s) involved PmpD-N-induced Toll-like receptors (TLRs), as well interleukin (IL)-6 IL-10 cytokine secretions. Thus, HD11 were treated with exogenous intracellular PmpD-N C. psittaci. chlamydial growth was by...
Contagious ecthyma is a highly contagious viral disease with zoonotic significance caused by orf virus (ORFV) that affects domestic, ruminants and humans. Live attenuated tissue culture vaccines are widely used in the fight against ORFV, however, conventional vaccine strains have many drawbacks. The aim of this project was to construct promising strain safety, high protection efficacy accessibility genetic manipulation disease. Using natural ORFV-GS14 as parental virus, recombinant...
Chlamydia psittaci ( C. ) is a common zoonotic agent that affects both poultry and humans. Interleukin 10 (IL-10) an anti-inflammatory factor produced during chlamydial infection, while dendritic cells (DCs) are powerful antigen-presenting induce primary immune response in the host. However, IL-10 DCs regulatory mechanisms infection remain elusive. In vivo vitro investigations of were performed. −/− mice, conditional depletion mice (zinc finger cell-diphtheria toxin receptor [zDC-DTR]),...
Interferon-stimulated gene product 15 (ISG15), a ubiquitin-like molecule, can be conjugated to protein substrates through reversible process known as ISGylation. ISG15 and ISGylation are both strongly upregulated by type I interferons play putative key roles in host innate immunity against viral infection. However, the function of identities largely unknown. Here, novel monoclonal antibody (Mab) that specifically recognizes porcine (pISG15) was employed capture ISG15-conjugated proteins from...
As a multifactorial cause, gastric ulceration-mediated diarrhea is widely prevalent in the weaned piglets, impairing pig health and economic benefits. With full implementation of antibiotic stewardship programs China, Bacillus cereus (B. cereus) Aspergillus fumigatus (A. fumigatus) were identified frequently porcine feedstuffs feeds animal industry. Association between feed-borne B. frequent remains unclear. In present study, we conducted survey A. from farms during hot season....
In this study, we report the establishment and characterization of a new epithelial cell line, goose embryonated line (GEE), derived from embryonic tissue. The purified GEE can efficiently grow over 65 passages in M199 medium supplemented with 10% fetal bovine serum at 37°C. Immunofluorescence assay was used to identify cells as by detecting expression Keratin-18 -19. Further characterizations demonstrated that be continuously subcultured (i) high capacity replicate for passages, (ii)...
Eimeria necatrix, an apicomplexan protozoa of the genus Eimeria, causes intestinal coccidiosis that can reduce growth performance poultry and result in high mortality older chickens. In this report, whole sporozoite proteins E.necatrix were studied by two-dimensional electrophoresis (2-DE) Western blotting using hyper-immune chicken serum containing E.necatrix-specific antibodies. Approximately 680 protein spots for detected 2-DE with silver staining, where 98 cross-reacted E....
Avian leukosis caused by avian virus (ALV), belonging to the genus Alpharetrovirus of family Retroviridae, is associated with benign and malignant tumors in hemopoietic cells poultry. Although several methods have been developed for ALV detection, most them are not suitable rapid on-site testing due instrument limitations, professional operators, or low sensitivity method. Herein, we described real-time recombinase polymerase amplification (RPA) assay detection subgroup J (ALV-J). The major...
Granulocyte-macrophage colony stimulating factor (GM-CSF), participates in diverse biological processes associated with innate and adaptive immunity, has unknown effects during PRRSV infection. Here, a double-antibody sandwich ELISA for pGM-CSF was developed in-house evaluation of level infection both vitro vivo . In assay, it notable that PRRSV-infected porcine alveolar macrophages (PAMs) yielded inconsistent protein- mRNA-level, suggesting post-transcriptional inhibition mRNA employed by...
Abstract Haemophilus parasuis is the causative agent of Glässer's disease and a major source economic losses in swine industry each year. To enhance production an inactivated vaccine against H. , availability nicotinamide adenine dinucleotide (NAD) must be carefully controlled to ensure sufficiently high cell density . In present study, real‐time viable was calculated based on capacitance culture. By assessing relationship between density/NAD concentration, NAD supply rate could adjusted...
Abstract Qu, G., Fu, S., Shen, N., Wang, J., Xiao, Y., Guan, Tang, Chen, L., Gao, S. and Z. 2010. Rapid sensitive diagnosis of porcine parvovirus by loop-mediated isothermal amplification (LAMP) method. J. Appl. Anim. Res., 37: 113–116. To form a detection model for the (PPV), six special primers were designed based on PPV gene NS1. After optimization only 45 min needed to amplify DNA incubation at 63C. The specificity LAMP assay was validated absence any cross-reaction with other...
Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that can cause meningitis both in pigs and human beings. However, the pathogenesis of central nervous system (CNS) infection caused by SS2 have not yet been elucidated. To find key molecules cerebrospinal fluid (CSF) needed for pathogenesis, meningoencephalitic pig model non-meningoencephalitic were established this study. CSF was collected from infected piglets, protein profiling performed with label-free proteomics technology. A...