A5100684383- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Plant Gene Expression Analysis
- Advanced Photocatalysis Techniques
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Cancer Treatment and Pharmacology
- Seaweed-derived Bioactive Compounds
- Environmental and Agricultural Sciences
- Nanomaterials for catalytic reactions
- Enzyme Catalysis and Immobilization
- Gas Sensing Nanomaterials and Sensors
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Maternal and fetal healthcare
- Flavonoids in Medical Research
- Environmental Changes in China
- Pregnancy and preeclampsia studies
- Advanced battery technologies research
- Selenium in Biological Systems
- Natural product bioactivities and synthesis
- Medicinal Plants and Bioactive Compounds
- Adsorption and biosorption for pollutant removal
- Environmental Quality and Pollution
First Affiliated Hospital of Anhui Medical University
2022-2025
Anhui Medical University
2022-2025
Tianjin Institute of Industrial Biotechnology
2014-2025
Chinese Academy of Sciences
2014-2025
Shanxi Medical University
2024-2025
Hubei University of Technology
2025
University of Chinese Academy of Sciences
2020-2024
University of Aberdeen
2024
Intelligent Synthetic Biology Center
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2022-2023
The flavonoid extract from Erigeron breviscapus, breviscapine, has increasingly been used to treat cardio- and cerebrovascular diseases in China for more than 30 years, plant supply of E. breviscapus is becoming insufficient satisfy the growing market demand. Here we report an alternative strategy breviscapine by building a yeast cell factory using synthetic biology. We identify two key enzymes biosynthetic pathway (flavonoid-7-O-glucuronosyltransferase flavone-6-hydroxylase) genome engineer...
Kaempferol is a flavonol with broad bioactivity of anti-oxidant, anti-cancer, anti-diabetic, anti-microbial, cardio-protective and anti-asthma. Microbial synthesis kaempferol promising strategy because the low content in primary plant source. In this study, biosynthesis pathway was constructed budding yeast Saccharomyces cerevisiae to produce de novo, several biological measures were taken for high production. Firstly, efficient synthases (FLS) from Populus deltoides introduced into...
Taxus, commonly known as yew, is a well-known gymnosperm with great ornamental and medicinal value. In this study, by assembling chromosome-level genome of the Himalayan yew (Taxus wallichiana) 10.9 Gb in 12 chromosomes, we revealed that tandem duplication acts driving force gene family evolution genome, resulting main genes for paclitaxel biosynthesis, i.e. those encoding taxadiene synthase, P450s, transferases, being clustered on same chromosome. The may also provide genetic resources...
Paclitaxel is a famous chemotherapeutic agent, but its microbial production poses long-standing challenge due to poor product selectivity. Taxadiene-5α-hydroxylase (CYP725A4) plays crucial role in the biosynthesis of paclitaxel, catalyzing oxidation taxadiene and iso-taxadiene. This process yields several products, including byproducts 5(12)-oxa-3(11)-cyclotaxane (OCT) 5(11)-oxa-3(11)-cyclotaxane (iso-OCT), as well target compound taxadien-5α-ol (T5OH). Despite extensive studies, molecular...
Salidroside (1) is the most important bioactive component of Rhodiola (also called as "Tibetan Ginseng"), which a valuable medicinal herb exhibiting several adaptogenic properties. Due to inefficiency plant extraction and chemical synthesis, supply salidroside currently limited. Herein, we achieved unprecedented biosynthesis from glucose in microorganism. First, pyruvate decarboxylase ARO10 endogenous alcohol dehydrogenases were recruited convert 4-hydroxyphenylpyruvate (2), an intermediate...
Diosgenin, mainly produced by Dioscorea species, is a traditional precursor of most hormonal drugs in the pharmaceutical industry. The mechanisms that underlie origin and evolution diosgenin biosynthesis plants remain unclear. After sequencing whole genome zingiberensis, we revealed evolutionary trajectory biosynthetic pathway demonstrated de novo yeast cell factory. First, found P450 gene duplication neo-functionalization, driven positive selection, played important roles pathway....
Cytochrome P450s are widespread in nature and play key roles the diversification functional modification of plant natural products. Over last few years, there has been remarkable progress identification with rapid development sequencing technology, "omics" analysis synthetic biology. However, challenges still persist respect crystal structure, heterologous expression enzyme engineering. Here, we reviewed several research hotspots P450 enzymes involved biosynthesis products, including...
The number of individuals with dementia is increasing, which negatively affects families, communities, and health care systems worldwide. changes in the incidence mortality due to Alzheimer's disease other forms at national level China have remained unknown over past three decades.Following general analytical strategy used Global Burden Disease Study (GBD) 2019, age- sex-specific rates for were analyzed. Trends from 1990 2019 assessed by joinpoint regression analysis. effects age, period...
Although cytochrome P450 enzymes are the most versatile biocatalysts in nature, there is insufficient comprehension of molecular mechanism underlying their functional innovation process. Here, by combining ancestral sequence reconstruction, reverse mutation assay, and progressive forward accumulation, we identified 5 founder residues catalytic pocket flavone 6-hydroxylase (F6H) proposed a “3-point fixation” model to elucidate mechanisms P450s nature. According this design principle pocket,...
ABSTRACT Age estimation is important in criminal investigations and forensic practice, extensive studies have focused on age determination based DNA methylation (DNAm) miRNA markers. Interestingly, it has been reported that combining different types of molecular omics data helps build more accurate predictive models. However, few compared the application combined DNAm to predict same cohort. In this study, a novel multiplex droplet digital PCR (ddPCR) system allows for simultaneous detection...
Abstract Background Taxus wallichiana is an important species for paclitaxel production. Previous genome versions spp. have been limited by extensive gaps, hindering the complete annotation and mining of (known as Taxol commercially) synthesis pathway-related genes. Results Here, we present first phased high-quality reference T. wallichiana, which significantly improves assembly quality corrects large-scale errors in previous versions. The 2 haplotypes are 9.87 Gb 9.98 length, respectively,...