A pixel-level colorimetric absorbance analysis can measure the total quantity of dye on paper, irrespective shape and size.

There are a large number of surface analytical methods, each with their own strengths and limitations. This review provides tools an amalgamated source information to those new characterization or who would like select the most appropriate, commonly accessible, technique for sample. It includes quick selection guide narrowing down suitable methods obtaining range compositional, structural, properties. Considerations recommendations approaching instrument technicians field experts also...

Herein we demonstrate the use of a wearable device that can selectively capture two distinct circulating protein biomarkers (recombinant P. falciparum rPfHRP2 and total IgG) from intradermal fluid live mice in situ, for subsequent detection vitro. The comprises microprojection array that, when applied to skin, penetrates outer skin layers interface directly with fluid. Because complexity biological being sampled, investigated effects solution conditions on attachment antibodies, optimize...

Development of antifouling films which selectively capture or target proteins interest is essential for controlling interactions at the "bio/nano" interface. However, in order to synthesize biofunctional from synthetic polymers that incorporate chemical "motifs" surface immobilization, antifouling, and oriented biomolecule attachment, multiple reaction steps need be carried out solid/liquid EKx a zwitterionic peptide has previously been shown have excellent properties. In this study, we...

Continuous monitoring using nanoparticle-based sensors has been successfully employed in complex biological systems, yet the still suffer from poor long-term stability partially because of scaffold materials chosen to date. Organosilica core-shell nanoparticles containing a mixture covalently incorporated pH-sensitive (shell) and pH-insensitive (core) fluorophores is presented as continuous pH sensor for application media. In contrast previous studies focusing on similar materials, we sought...

Optical nanosensors for the detection of polyions, including protamine and heparin, have to date relied upon ion-exchange reactions involving an analyte optical transducer. Unfortunately, due limited selectivity available ionophores this mechanism has suffered from severe interference in complex sample matrices. To no polyion demonstrated acceptable performance serum, plasma or blood. Herein we describe a new type nanosensor based on our discovery "hyper-polarizing lipophilic phase" which...

Selective capture of disease-related proteins in complex biological fluids and tissues is an important aim developing sensitive protein biosensors for vivo applications. Microprojection arrays are biomedical devices whose mechanical chemical properties can be tuned to allow efficient penetration skin, coupled with highly selective biomarker from the environment skin tissue. Herein, authors describe improved surface modification strategy produce amine-modified polycarbonate arrays, followed...

In ion sensing applications, selective ion-receptor complexation is the molecular basis for endowing material with selectivity. this work, thin polymeric membrane-based transfer voltammetry used to investigate complexation, using a range of electrically neutral ionophores and surfactants as examples. Previous studies lacked convincing approach eliminate influence from transducing layer, resulting in deviations observed binding constants compared potentiometric methods. A recently developed...

Nanoparticle-cell interactions between silica nanomaterials and mammalian cells have been investigated extensively in the context of drug delivery, diagnostics, imaging. While there are also opportunities for applications infectious disease, nanoparticles with pathogenic microbes relatively underexplored. To bridge this knowledge gap, here, we investigate effects organosilica different sizes, concentrations, surface coatings on association viability major human fungal pathogen Candida...

The only alloy currently utilized for additive manufacture of bone implants, Ti–6Al–4V, has a high elastic modulus and bioinert surface, potentially inducing stress shielding hindering osseointegration. Low-modulus materials with bioactive surfaces could significantly reduce implant failure rates by improving the interaction between implants surrounding bone. In this study, laser powder bed fusion Ti25Ta Ti65Ta alloys, highlighted previously their low moduli, were assessed surface osteogenic...

Point-of-care quantification of the anticoagulant heparin still remains a significant clinical challenge as reference method (colorimetric anti-factor Xa assay) cannot be performed in whole blood. Our group recently put forth novel optical nanosensing principle using an ionic solvatochromic dye signal transducer. These nanosensors demonstrated significantly improved selectivity and sensitivity compared to ion-exchange-type polyion enabled protamine/heparin blood plasma samples. However,...

We compare here the effect of surfactants on ion-selective membranes measured via voltammetry and optode emulsions optically. Cyclic a thin-film membrane is shown to be useful screening technique for estimation effective complex formation constants selectivity coefficients different with various cations. This particularly its ability identify separate ion-transfer events (free, surfactant complexed, ionophore complexed) specific membrane. However, we also caution against over-reliance this...

Recent work has shown that ion-selective components may be transferred from nanoemulsions (NEs) to endow polymeric membranes with sensing properties. This approach also been used for nanopipette electrodes achieve single-entity electrochemistry, thereby the response of single adhered nanospheres. To this date, however, mechanism and rate component transfer remain unclear. We study here lipophilic ionic compounds into thin plasticized poly(vinyl chloride) (PVC-DOS) films by chronoamperometry...

Heparin quantification at the point of care has been medical interest for years but a suitable measurement method whole blood is still elusive. Our group recently developed nanoparticle-based optical sensor protamine that allows heparin in plasma. This work discusses effect transducing-dye structure and promise embedding sensors an agarose gel avoiding red cell interference.

Immunoassays are ubiquitous across research and clinical laboratories, yet little attention is paid to the effect of substrate material on assay performance characteristics. Given emerging interest in wearable immunoassay formats, investigations into materials that provide an optimal mix mechanical bioanalytical properties paramount. In course our developing immunoassays which can penetrate skin selectively capture disease antigens from underlying blood vessels, we recently identified...

The last decade has witnessed a rapid development of nano- and microparticle-based optical ion sensors, including ion-selective optodes (ISOs). While the application nano-ISOs shown promising performance for sensing inorganic ions, polyion using nanoscale ISOs encountered significant interference in complex samples such as blood plasma. Recently, we have reported on new principle that operates through novel mechanism to overcome this challenge. showed improved characteristics not observed...

Abstract Ionophore‐based carbonate‐selective sensors have become reliable analytical tools due to their high selectivity and versatility of applications. Nonetheless, successful examples implementation in nanoscale devices are yet be found the literature. Herein we describe first attempt develop carbonate with ionophores using ion‐selective nanoemulsions. Classical chromoionophore‐based were shown perform poorly upon transfer nanoscale. We hypothesize that this is caused by heightened...