Mykola Sushko

ORCID: 0000-0003-2723-9903
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About
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Research Areas
  • Viral Infections and Vectors
  • Animal Virus Infections Studies
  • Global Peace and Security Dynamics
  • Animal Disease Management and Epidemiology
  • Viral Infections and Immunology Research
  • Microbial infections and disease research
  • Vector-Borne Animal Diseases
  • Virus-based gene therapy research
  • Influenza Virus Research Studies
  • Viral gastroenteritis research and epidemiology
  • Yersinia bacterium, plague, ectoparasites research
  • Virology and Viral Diseases
  • Environmental and Biological Research in Conflict Zones
  • Vector-borne infectious diseases
  • Travel-related health issues
  • Cancer Genomics and Diagnostics
  • Agriculture and Biological Studies
  • Zoonotic diseases and public health
  • Single-cell and spatial transcriptomics
  • Plant and Fungal Interactions Research
  • Genetics, Bioinformatics, and Biomedical Research
  • Respiratory viral infections research

Here, we report the complete genome sequence of an African swine fever (ASF) virus (ASFV/Kyiv/2016/131) isolated from spleen a domestic pig in Ukraine with lethal case fever. Using only long-read Nanopore sequences, assembled full-length 191,911 base pairs single contig.

10.1128/mra.00883-19 article EN Microbiology Resource Announcements 2019-10-16

Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For non-enveloped virus with small genome (1.7 kb ssDNA), PCV2 remarkably diverse, eight genotypes (a–h). New spread through the migration wild boar, which are thought to infect pigs and further trade. Despite large swine population, diversity in Ukraine has been under-sampled, few sequences reported past decade. To gain deeper...

10.3390/v14050924 article EN cc-by Viruses 2022-04-28

Emerging RNA virus infections are a growing concern among domestic poultry industries due to the severe impact they can have on flock health and economic livelihoods. Avian paramyxoviruses (APMV; avulaviruses, AaV) pathogenic, negative-sense viruses that cause serious in respiratory central nervous systems. APMV was detected multiple avian species during 2017 wild bird migration season Ukraine studied using PCR, isolation, sequencing. Of 4090 samples collected, mostly from southern Ukraine,...

10.3390/v15030699 article EN cc-by Viruses 2023-03-08

African swine fever (ASF) is a viral disease, endemic to Africa, that causes high mortality when introduced into domestic pig populations. Since the emergence of p72-genotype II virus (ASFV) in Georgia 2007, an ASF epidemic has been spreading across Europe and many countries Asia. The first reached Ukraine 2012. To better understand dynamics spread Ukraine, we analyzed spatial temporal outbreak data reported between 2012 mid-2023. highest numbers outbreaks were 2017 (N = 163) 2018 145), with...

10.3390/vaccines11071145 article EN cc-by Vaccines 2023-06-25

Porcine circovirus type 2 (PCV2) is responsible for a number of porcine associated diseases (PCAD) that can severely impact domestic pig herds. For non-enveloped virus with small genome (1.7 kb ssDNA), PCV2 remarkably diverse, 8 subtypes (a-h). New spread through migration wild boars, which are thought to infect pigs and further the trade. Despite large swine population, diversity in Ukraine has been undersampled, few sequences reported past decade. To gain deeper understanding subtype...

10.20944/preprints202204.0050.v1 preprint EN 2022-04-07

Highly pathogenic avian influenza viruses (HPAIV) can be carried long distances by migratory wild birds and poultry trade. (HPAI) is often lethal in domestic sporadically infect cause severe respiratory or systemic disease other species including humans. Since 2003, the H5 subtype of HPAIV have spread from epicenters China to neighboring regions East Southeast Asia, across Central Asia Indian subcontinent, Europe, Africa, North America. Outbreaks H5N1 struck Ukraine 2005. In 2016, A H5N8...

10.1089/vbz.2021.0031 article EN Vector-Borne and Zoonotic Diseases 2021-12-01

Державний науково-дослідний інститут з лабораторної діагностики та ветеринарно-санітарної експертизи РЕЗУЛЬТАТИ ЛАБОРАТОРНОГО КОНТРОЛЮ ДЕЯКИХ ІНФЕКЦІЙНИХ ХВОРОБ ТВАРИН В УКРАЇНІ У 2018 РОЦІ МОЛЕКУЛЯРНО-ГЕНЕТИЧНИМИ МЕТОДАМИ

10.31073/vet_biotech34-16 article UK Bulletin Veterinary biotechnology 2019-01-01

Next-Generation Sequencing (NGS), also known as high-throughput sequencing, refers to a set of modern DNA sequencing technologies that have revolutionized the field genomics. Advantages NGS techniques involving high speed (parallel is faster than traditional methods, allowing researchers obtain results more quickly), cost-effectiveness (ability sequence multiple fragments simultaneously reduces cost per base compared sequencing), and scalability (platforms can be scaled accommodate varying...

10.31073/onehealthjournal2024-i-05 article EN cc-by-nc-nd One Health Journal 2024-03-06

Purpose: Avian influenza (AI) diagnostics is an important part of the veterinary preventive measures to control this disease. In Ukraine, AI detection performed using virological and molecular genetic methods in accordance with OIE recommendations. The purpose work was toassess capacity expanding usage surveillance system within Ukraine. Methods & Materials: During period from 2017 Q1 2018, 4390 biological material samples were analyzed real-time PCR, 81126 blood serum Hemagglutination...

10.1016/j.ijid.2018.11.240 article EN cc-by-nc-nd International Journal of Infectious Diseases 2019-01-30

Emerging RNA virus infections are a growing concern among domestic bird and poultry industries due to the severe impact it can have on flock health economic livelihoods. Avian paramyxoviruses (APMV) pathogenic, negative sense viruses that cause serious in respiratory central nervous system. APMV was detected multiple avian species during 2017 migration season Ukraine, studied using PCR, isolation, sequencing. Of 4090 wild samples, eleven swabs were isolated chicken embryos identified for...

10.20944/preprints202206.0322.v1 preprint EN 2022-06-23

The article presents the results of studying prevalence and species composition ticks in southern south-eastern regions Ukraine.During 2019-2021 15644 were collected Odesa, Kherson, Mykolaiv, Zaporizhzhia Kharkiv regions.Three identified: Dermacentor marginatus -7230 individuals (46.2% all ticks), Ixodes ricinus -5317 (34.0%) Hyalomma marginatum -3097 (19.8%).It has been established that is dominant among found Odesa (68.0% total number region), Kherson (50.8%),Zaporizhzhia (41.4%) (88.1%)...

10.31073/vet_biotech42-15 article EN Bulletin Veterinary biotechnology 2023-01-01

New tests for the detection and typing of animal pathogens have been developed veterinary medicine. Careful systematization is required to determine place molecular-based tools’ applications in existing system epizootological epidemiological surveillance. Today, molecular genetic tests, including PCR, are used medicine agriculture following purposes:- surveillance diagnosis infectious certain invasive diseases, - pathogens, study their eco-geographic features, drift variability evolution,...

10.31073/onehealthjournal2023-iv-06 article EN cc-by-nc-nd One Health Journal 2023-12-20

The aim of the work was to analyze current epidemic situation on highly pathogenic avian influenza worldwide and in Ukraine during 2019–2020. research conducted framework state control infectious animal diseases. results PCR tests obtained State Scientific Research Institute Laboratory Diagnostics Veterinary Sanitary Expertise regional laboratories, data OIE website (http://www.oie.int) 2019 2020 were analyzed summarized. During 2019–2020 outbreaks detected 53 countries World: Europe — 22,...

10.36016/jvmbbs-2021-7-1-2-2 article EN cc-by-nc-nd Journal for Veterinary Medicine Biotechnology and Biosafety 2021-06-24
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