A5025655248- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Mycorrhizal Fungi and Plant Interactions
- Bacillus and Francisella bacterial research
- Aquaculture disease management and microbiota
- Forest Ecology and Biodiversity Studies
- Brucella: diagnosis, epidemiology, treatment
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Fungal Biology and Applications
- vaccines and immunoinformatics approaches
- Diagnosis and treatment of tuberculosis
- Food Safety and Hygiene
- Polar Research and Ecology
- RNA and protein synthesis mechanisms
- Liver Disease and Transplantation
National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine"
2014-2023
National Academy of Agrarian Sciences of Ukraine
2020
Our study demonstrates the repeated isolation of vaccine-derived Newcastle disease viruses from different species wild birds across four continents 1997 through 2014. The data indicate that at least 17 ten avian orders occupying habitats excrete viruses. most frequently reported isolates were detected among individuals in order Columbiformes (n = 23), followed frequency by Anseriformes 13). Samples isolated both free-ranging 47) and kept captivity 7). number recovered corresponded with...
Newcastle disease virus (NDV) infects a wide range of bird species worldwide and is importance to the poultry industry. Although certain genotypes are clearly associated with wild species, role those in movement viruses migratory routes they follow still unclear. In this study, we performed phylogenetic analysis nineteen NDV sequences that were identified among 21,924 samples collected from synanthropic birds different regions Ukraine 2006 2015 compared them isolates other continents. birds,...
Here, we report the complete genome sequence of an African swine fever (ASF) virus (ASFV/Kyiv/2016/131) isolated from spleen a domestic pig in Ukraine with lethal case fever. Using only long-read Nanopore sequences, assembled full-length 191,911 base pairs single contig.
Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For non-enveloped virus with small genome (1.7 kb ssDNA), PCV2 remarkably diverse, eight genotypes (a–h). New spread through the migration wild boar, which are thought to infect pigs and further trade. Despite large swine population, diversity in Ukraine has been under-sampled, few sequences reported past decade. To gain deeper...
ABSTRACT Here, we report the complete genome sequence of a virus putative new serotype avian paramyxovirus (APMV). The was isolated from white-fronted goose in Ukraine 2011 and designated goose/Ukraine/Askania-Nova/48-15-02/2011. genomic characterization isolate suggests that it represents novel group APMV 13.
The Ukraine ranks among the top 20 countries with highest number of multidrug-resistant (MDR) and extensively drug resistant (XDR) Mycobacterium tuberculosis cases in world. However, little is known genetic diversity, i.e., resistance signatures, clinical isolates from this region. We analyzed seven most prevalent MDR/XDR antibiotic resistance-conferring genes (n = 75) collected geographically diverse Ukrainian oblasts southern Crimean peninsula. Genomic analysis revealed that 6 (8%) were...
The complete genome of Salmonella enterica subsp. serovar Kottbus (serogroup C2-C3), which was isolated from a commercial pork production facility in Kharkiv, Ukraine, assembled using long-read Nanopore sequences. A single circular contig (4,799,045 bp) comprised chromosome encoding antibiotic resistance, highlighting the risk cross-species livestock and human infection.
Abstract A Knowledge, Attitudes, and Practices (KAP) questionnaire was designed to collect information on farmers’ knowledge of ASF their practices surrounding that could impact the spread disease. The distributed, data collected, from 233 backyard farmers five selected Oblasts (Rivne, Kharkiv, Odessa, Zakarpattia Kiev). Kruskal‐Wallis tests were conducted identify factors influence knowledge, Dunn performed determine differences between groups when significant. Spearman carried out explore...
The complete genome sequence of Brucella abortus 68, isolated from an aborted sheep fetus in Luhansk, Ukraine, was assembled using Nanopore sequences. Two circular chromosomes totaling 3,281,317 bp ( N 50 , 2,124,943 bp) comprised the sequence. strain encodes fosfomycin antibiotic resistance gene FosX highlighting risk cross-species livestock and human infection.
Genotypic variation in Beijing lineages of Mycobacterium tuberculosis (MTB), the causative agent (TB), has been associated with hyper virulence and spread extensively multiple drug (X/MDR) resistant MTB strains Eastern Europe, Central Asia, East Asia. The clinical outcomes 215 new cases TB among population Kharkiv region Ukraine were analyzed to uncover factors severe infection. Infecting profiled by 5 locus exact tandem repeats (ETRs) 15 mycobacterial interspersed repetitive unit-variable...
Background: Avian paramyxoviruses (APMVs) belong to the genus Avulavirus of family Paramyxoviridae and have been shown infect a wide variety poultry wild bird species. Up date, International Committee on Taxonomy viruses officially approved 9 serotypes APMV (APMV 1 – 9), but recently four new described 10 13). A member putative serotype 13 was isolated from white-fronted goose territory Askania-Nova National Park (Ukraine) in 2011. Methods & Materials: The virus propagated...
Porcine circovirus type 2 (PCV2) is responsible for a number of porcine associated diseases (PCAD) that can severely impact domestic pig herds. For non-enveloped virus with small genome (1.7 kb ssDNA), PCV2 remarkably diverse, 8 subtypes (a-h). New spread through migration wild boars, which are thought to infect pigs and further the trade. Despite large swine population, diversity in Ukraine has been undersampled, few sequences reported past decade. To gain deeper understanding subtype...
Tularaemia is a zoonotic disease caused by the gram-negative bacterium Francisella tularensis, which endemic to Ukraine. The aim of this work was provide screening different field samples (rodent tails, ticks, pellets, water, and hay) obtain an actual picture tularaemia epizootic situation in Kharkiv, Dnipropetrovsk, Mykolaiv oblasts.Samples were collected using flag method (for ticks) break-back traps rodents). Also, hay, water owl pellets for study. F. tularensis genetic material detected...
For the first time in Ukraine we confirmed canine brucellosis caused by Brucella canis. The bacterium was isolated from testicles of three-year-old male Labrador retriever with orchitis and epididymitis. Initially blood serum sample positive cCFT, AGID LFIA. In addition to pathogen isolation identification biochemical test PCR, antimicrobial susceptibility performed that showed sensitive B. canis commonly used antibiotics, which should be taken into account for further therapy
Aim. This study was focused on (i) detection of specifi c BVDV-antibodies within selected cattle farms, (ii) identifi cation persistently infected (PI) animals and (iii) genetic typing BVDV isolates. Methods. RNA extraction, real-time polymerase chain reaction, ELISA technique, sequencing. Results. Specifi BVDV- antibodies were detected in 713 1,059 analyzed samples (67.3 per cent). number is agreement with fi ndings many herds around the world. However, positive differed herds. While 57 out...
The <b>aim</b> of our study was to features the treatment outcome patients with multidrug-resistant tuberculosis (TB) and Beijing lineage 2 (BL2) MTB. <b>Methods:</b> This is a prospective, cohort all culture-confirmed pulmonary TB MTB resistance at least isoniazid rifampicin. Bacterial DNA isolated from 201 patients. Isolates were sequenced on Illumina platforms using paired-end library design. Following complete genome sequencing, we analyzed reads Profiler (version 3.0.6) software compile...
The development of effective complex drugs for increasing the viability young farm animals by stimulating activity immunity, studying biological effects these on expression cytokine genes and formation humoral immunity its markers is an urgent problem. aim research was to determine effect probiotic-metal-protein preparation (PNMGP) dynamics innate factors in intact chickens when they are vaccinated with a live vaccine against Newcastle disease. carried out at NSC "Institute Experimental...
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