A5074193548- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Alzheimer's disease research and treatments
- Genetics and Neurodevelopmental Disorders
- Muscle Physiology and Disorders
- Bipolar Disorder and Treatment
- Pancreatic function and diabetes
- Cardiovascular and exercise physiology
- Cholinesterase and Neurodegenerative Diseases
- Ion channel regulation and function
- Diet and metabolism studies
- Muscle activation and electromyography studies
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and biosynthesis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Genetic Syndromes and Imprinting
- Nutritional Studies and Diet
- Neurogenetic and Muscular Disorders Research
Brock University
2017-2022
Neuronatin (NNAT) is a transmembrane protein in the endoplasmic reticulum involved metabolic regulation. It shares sequence homology with sarcolipin (SLN), which negatively regulates sarco(endo)plasmic Ca 2+ ‐ATPase (SERCA) that maintains energy homeostasis muscles. Here, we examined whether NNAT could uncouple transport activity of SERCA from ATP hydrolysis, similarly to SLN. significantly reduced uptake without altering activity, ultimately lowering apparent coupling ratio SERCA. This...
Sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) uncoupling in skeletal muscle and mitochondrial via protein 1 (UCP1) brown/beige adipose tissue are two mechanisms implicated energy expenditure. Here, we investigated the effects of glycogen synthase kinase 3 (GSK3) inhibition lithium chloride (LiCl) treatment on SERCA UCP1 expression adipose. C2C12 3T3-L1 cells treated with LiCl had increased levels, respectively, ultimately raising cellular respiration; however, this was only observed when...
Lithium, a commonly used treatment for bipolar disorder, has been shown to have neuroprotective effects other conditions including Alzheimer's disease via the inhibition of enzyme glycogen synthase kinase-3 (GSK3). However, dose-dependent adverse lithium are well-documented, highlighting need determine if low doses can reliably reduce GSK3 activity.The purpose this study was evaluate low-dose supplementation on activity in brain an early, diet-induced model.Male C57BL/6J mice were divided...
Sporadic Alzheimer’s disease (AD) is a neurodegenerative characterized by neurofibrillary tangles composed of hyperphosphorylated tau proteins and amyloid‐beta (Aβ) plaques. Together, these two histopathological detriments in the brain lead to progressive cognitive decline. Glycogen synthase kinase‐3 β (GSK3β), kinase found abundantly brain, has been implicated development AD pathologies. GSK3β remains constituently active until phosphorylated at its inhibitory Ser9 residue. When active,...
Promotion of oxidative metabolism in skeletal muscle improves performance, fatigue resistance, and may help combat the obesogenic effects a high fat diet. Lithium is well-known glycogen synthase kinase (GSK3) inhibitor we have recently shown that low-dose lithium supplementation (10 mg kg-1 day-1) augments capacity mice. In soleus muscle, mediated GSK3 inhibition increased expression markers such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) myosin heavy...
Neuronatin (NNAT) is a paternally imprinted gene involved in many aspects of metabolism but the underlying cellular mechanism remains unknown. Its sequence homology with phospholamban (PLN) and sarcolipin (SLN) suggest that NNAT has putative role regulating enzymatic activity sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) striated muscles. Both PLN SLN physically interact functionally inhibit SERCA by lowering its affinity for Ca2+, only unique ability to uncouple SERCA-mediated Ca2+...