The taxonomic position of a dibenzothiophene-desulphurizing soil actinomycete was established using polyphasic approach. organism, strain IEGMT, shown to have chemical and morphological properties typical members the genus Gordonia. tested formed distinct phyletic line within evolutionary radiation occupied by Gordonia, with Gordonia alkanivorans DSM 44369T, desulfuricans NCIMB 40816T rubropertincta 43197T as most closely related organisms. Strain IEGMT has range phenotypic that distinguish...

Patient-specific absorbed dose calculations for molecular radiotherapy require accurate activity quantification. This is commonly derived from Single-Photon Emission Computed Tomography (SPECT) imaging using a calibration factor relating detected counts to known in phantom insert.A series of inserts, based on the mathematical models underlying many clinical dosimetry calculations, have been produced 3D printing techniques. SPECT/CT data inserts has used calculate new organ-specific factors...

The taxonomic position of two actinomycetes isolated from soil was established using a polyphasic approach. organisms, designated 213ET and 213F, were found to have chemical morphological properties consistent with their assignment the genus Gordonia. Nearly complete sequences 16S rDNA genes strains determined following isolation direct sequencing amplified genes. tested identical formed phylogenetic line within evolutionary radiation occupied by Gordonia that most closely related...

The weak acid 4-nitrophenol (NP) experiences a large (ca. 2 unit) increase in pKa when solubilized buffered water-in-oil (w/o) microemulsions stabilized by the surfactant sodium bis-2-ethylhexylsulphosuccinate (AOT), as compared with aqueous solution value (pK0a= 7.0 at 25 °C). In addition both phenol (NPH) and its conjugate base (NP–) undergo hypsochromatic shift of UV–VIS spectrum, relative to that solution, low water contents. contrast, 4-nitrophenyl-2-sulphonate, structural analogue NP...

The optimised delivery of Molecular Radiotherapy requires individualised calculation absorbed dose to both targeted lesions and neighbouring healthy tissue. To achieve this, accurate quantification the activity distribution in patient by external detection is vital. This work extends specific anatomy-related calibration true organ shapes. A set patient-specific 3D printed inserts based on a diagnostic CT scan was produced, comprising liver, spleen kidneys. were used calculate factors for...

Water-in-oil microemulsions are thermodynamically stable single-phase dispersions of water and surfactant within a continuous oil phase. The classical ternary system, based on the sodium bis(2-ethylhexyl)sulfosuccinate ('AOT'), an alkane such as n-heptane, is optically transparent monodispersion spherical water-droplets coated with close-packed monolayer droplet radius is, to good first approximation, directly proportional molar water: ratio, R. Enzymes dissolved in droplets retain activity...

Water-in-oil (w/o) microemulsions are thermodynamically stable dispersions of water droplets in an oil-continuous medium. Water and solutes exchange between a random process whereby pairs fuse transiently then separate again. An equation is derived for the rate irreversible chemical reaction two water-soluble reactants A B, initially isolated droplets, by applying steady-state approximation (i.e. assuming that concentration intermediate droplet species containing both generated fusion...

Changes in the enzymatic properties of horse liver alcohol dehydrogenase (HLADH; EC 1.1.1.1) were studied as a function incubation time Aerosol‐OT/isooctane microemulsions. The enzyme was characterized by fluorimetric binding studies inhibitor isobutyramide to binary complex, HLADH‐NADH and determination K m,app V max,app values for cyclohexanone. cyclohexanone d,app stay constant throughout 48‐h incubation, whereas total number sites decrease. Thus inactivation process previously described...

This work improves on breast cancer phantom materials for use in a terahertz imaging setup. Previously-developed solid-emulsion formulations based oil, water, and TX151 solidifying powder are updated to include agar margarine order improve the consistency of optical properties. Additionally, methodology using heating process is introduced remove excess air bubbles from imaging. Spectroscopy fibrous tissue phantoms shows good correlation properties range compared published values, reduction...

Obesity, type 2 diabetes, and heart disease are linked to an unhealthy diet. Sarco(endo)plasmic reticulum calcium (Ca2+) ATPase 2a (SERCA2a) controls cardiac function by transporting Ca2+ in cardiomyocytes. SERCA2a is altered diet acetylation, independently; however, it unknown if alters acetylation. Sirtuin (SIRT) 3 enzyme that might preserve health under conditions of macronutrient excess modulating metabolism via regulating deacetylation target proteins. Our objectives were determine...

Bilirubin oxidase can catalyse the oxidation of its primary substrate, bilirubin, in a water‐in‐oil microemulsion, which consists discrete nanometer‐diameter water droplets dispersed continuous water‐immiscible oil medium. The are stabilized by monolayer surfactant, cetyltrimethylammonium bromide present at oil/water interface. Spectroscopic evidence is presented to show that bilirubin solubilized this system located mainly surfactant layer, form accessible enzyme molecule. Studies on...

The infinite-dilution sedimentation coefficient, s020, of droplets formed in water-in-oil (w/o) microemulsions composed n-heptane, water and AOT increases with increasing molar ratio water:AOT, R, according to the empirical equation, s020= 5.7 + 0.983R+ 0.043R2(5 < R 50). derived water-pool radius, rwp, is related by rwp/nm = 0.54 0.15R, agreement direct size measurements using small-angle neutron X-ray scattering techniques. solubilisation properties these for α-chymotrypsin (αCT)...

Abstract Background Patient motion during rest-stress vasodilator rubidium-82 myocardial perfusion PET remains a challenge and can affect relative images interpretation when severe. Current commercial solutions are limited to frame-by-frame correction of dynamic for blood flow assessment. The time-averaged static challenge. Our department has previously published work on the severity prevalence degradation demonstrated usefulness prototype Data-Driven Motion Correction (DDMC) algorithm. We...

Sarco(endo)plasmic reticulum calcium (Ca2+) ATPase (SERCA) transports Ca2+ in muscle. Impaired SERCA activity may contribute to diabetic myopathy. Sirtuin (SIRT) 3 regulates muscle metabolism and function; however, it is unknown if SIRT3 or acetylation. We determined overexpression enhances mouse gastrocnemius preserves a model of type 2 diabetes, induced by high fat - sucrose (HFHS) feeding. also the acetylation status proteins altered HFHS Wild-type (WT) transgenic (SIRT3TG) mice,...

Background: Frailty and cardiovascular disease (CVD) share common pathophysiology in their progression. Declines bone health often occur concomitantly with frailty CVD. Therefore, analytes of metabolism may be useful biomarkers pre-frailty CVD risk. The aim this study was to identify the effects risk on systemic concentrations inflammatory middle-aged older females. Method: This case-control is a secondary analysis data from 1030 females no self-reported history measured using Fried...

Background Diabetes induces a distinct form of cardiomyopathy, termed diabetic cardiomyopathy (DCM). A key pathophysiological mechanism DCM is reduced level and activity sarco(endo)plasmic reticulum Ca 2+ ATPase 2a (SERCA2a). Recently, it has been discovered that the function SERCA2a negatively regulated by post‐translational modification, acetylation. The sirtuin (SIRT) isoform, SIRT3 full‐length (SIRT3 FL ), deacetylates protein substrates, though unknown if SERCA2a. Therefore, objective...