A5053811823- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Pluripotent Stem Cells Research
- Immunotherapy and Immune Responses
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Renal and related cancers
- Liver physiology and pathology
- Single-cell and spatial transcriptomics
Princess Máxima Center
2023-2025
Research Institute of Molecular Pathology
2023-2024
University of Trento
2020
Human brain development involves an orchestrated, massive neural progenitor expansion while a multi-cellular tissue architecture is established. Continuously expanding organoids can be grown directly from multiple somatic tissues, yet to date, solely established pluripotent stem cells. Here, we show that healthy human fetal in vitro self-organizes into (FeBOs), phenocopying aspects of vivo cellular heterogeneity and complex organization. FeBOs expanded over long time periods. FeBO growth...
Fibrolamellar carcinoma (FLC) is a lethal primary liver cancer, affecting young patients in absence of chronic disease. Molecular understanding FLC tumorigenesis limited, partly due to the scarcity experimental models. Here, we CRISPR-engineer human hepatocyte organoids recreate different backgrounds, including predominant genetic alteration, DNAJB1-PRKACA fusion, as well recently reported background FLC-like tumors, encompassing inactivating mutations BAP1 and PRKAR2A. Phenotypic...
Abstract The tumour microenvironment is programmed by cancer cells and substantially influences anti-tumour immune responses 1,2 . Within the microenvironment, CD8 + T undergo full effector differentiation acquire cytotoxic functions in specialized niches 3–7 Although interactions with type 1 conventional dendritic have been implicated this process 3–5,8–10 , underlying cellular players molecular mechanisms remain incompletely understood. Here we show that inflammatory monocytes can adopt a...
<h3>Background</h3> Immunotherapy has significantly improved the outcome of patients with metastatic melanoma. However, there is still an unmet need to develop rational combination therapies for who resistance after immunotherapy or cross-resistance prior targeted therapy. In solid tumors, tumor microenvironment (TME) a major determinant anti-tumor immunity and strongly influenced by cancer cells. <h3>Methods</h3> To investigate mechanisms immune evasion requirements functional T cell...