Abstract Background Studies on a new coronavirus disease (COVID-19) show the elevation of liver enzymes and fibrosis index (FIB-4) independently pre-existing diseases. It points to increased fibrogenesis during acute COVID-19 with possible long-term consequences. This study aimed assess in patients by serum hyaluronic acid (HA) FIB-4. Methods The included group (66 patients, 50% females, mean age 58.3 ± 14.6), post-COVID (58 3–6 months after recovery, 47% 41.2 13.4), control (17 people, 42.8...

Genetic control of immune reactions has a major role in the development rheumatic heart disease (RHD) and differs between patients with fever (RF). Some authors think risk acquiring RHD is associated HLA class II DR DQ loci, but other views exist, due to various HLA-typing methods ways grouping cases. Our goal was determine relations alleles or protection from RF relatively homogeneous clinical manifestations. A total 70 under age 18 years were surveyed Latvia. genotyping DRB1*01 DRB1*18...

The HLA system is being paid more and attention because it very significant in polymorphous immunological reactions. Several studies have suggested that genetic susceptibility to rheumatic fever (RF) heart disease (RHD) linked class II alleles. We hypothesized associations within RHD may be consistent if analysed amongst patients with a relatively homogeneous clinical outcome. A total of 70 RF under the age 18 years were surveyed Latvia. genotyping DQA1, DQB1 DRB1 was performed using PCR...

Juvenile idiopathic arthritis (JIA) is a heterogeneous condition and therapeutic strategies vary in different JIA types. The routinely accepted practice to start with Sulphasalazine (SS) as the first line treatment patients HLA B27 positive proves be ineffective large proportion of children.to investigate clinical characteristics, determined allele types their connection antirheumatic homogenous patient groups.56 diagnosed observed over period 2006 2009 included study. HLAB27 were using PCR...

Certain Enterobacteriaceae strains have been associated with the development of ulcerative colitis (UC). Extended-spectrum β-lactamase (ESBL)-producing are most commonly found multi-drug-resistant (MDR) bacteria colonizing gut in UC patients and might trigger a more severe disease activity patients.The aim this study was to evaluate whether is higher colonization ESBL-producing Enterobacteriaceae.A cross-sectional, pilot carried out tertiary medical center Latvia. Demographic data were...

Objective. The objective of this study was to investigate the prevalence HCV (hepatitis C virus) infection in hemophilia patients Latvia and analyze association between natural clearance human leukocyte antigen (HLA) class II genes. Material methods. From 61 hemophilic participating study, 38 were adults 23 pediatric younger than 18 years. To HLA alleles HCV, gene frequency compared group control 60 healthy subjects, all men. Serum RNA qualitatively determined identified by polymerase chain...

Many autoimmune diseases are associated with variants of HLA genes such as those encoding the MHC complex. This correlation is not absolute, but may help in understanding molecular mechanism disease. The purpose this study was to determine HLA-DR,-DQ alleles Latvian patients Lyme borreliosis and control (healthy) persons. Case subjects were similar age, gender ethnic heritage differed only regards presence Borrelia burgdorferi infection. included 25 clinical stage – erythema migrans 30...

The aim of this study was to determine the human leukocyte antigen (HLA)-DRB1 alleles in two groups patients Latvia: with Lyme borreliosis and neuroborreliosis. included 216 borreliosis, 29 neuroborreliosis 282 control persons. All surveyed persons were residents Latvia. HLA-DR genotyping performed by polymerase chain reaction- sequence specific primer (PCR-SSP). predisposition is associated HLA-DRB1*07, -DRB1*17(03), -DRB1*04, -DRB1*15(02) alleles. allele -DRB1*11(05), -DRB1*14(06)...

Temporomandibular joint (TMJ) arthritis is seen very often (38-87 %) in children with juvenile idiopathic (JIA). With contrast enhanced magnetic resonance imaging (MRI) we can detect more cases of TMJ than ever before. Previous studies show that HLA II class alleles may have protective or risk importance JIA subtypes. Our objective to identify and protection patients arthritis.During the period from 2010 2015 MRI for was performed 85 who were genotyped HLA- DRB1; DQB1 DQA1 using RT-PCR...

Background:The level of incidence Tick-borne disease in Latvia still is one the highest Europe.There are some similarities between viral agents, and HLA molecules, because organism develops way or another immune response to infection.Clarifying polymorphisms molecules will allow identify regularities pathological process develop a new approach treating these diseases.The purpose: Of this study was determine HLA-DR alleles two groups Latvian patients: patients with Lyme borreliosis (LB)...

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic with serious implications and open questions for all areas of medicine, including immunocompromised patients. In Latvia, as the end 2022, 229 new cases HIV were registered, reflecting an incidence rate 12.2 per 100,000 people. Despite fact that patients are at risk poor outcomes COVID-19, there is currently no evidence clinical manifestations COVID-19 in people living (PLWH) differ from those...

Abstract The aim of this study was to assess the main effects and interaction between viral hepatitis C (HCV) coinfection antiretroviral therapy (ART) by using a nonparametric ANOVA on direct indirect markers liver fibrosis in HIV-infected patients. sample included 178 HIV patients aged from 23 65 (36% females). following parameters were determined blood patients: hyaluronic acid, pro-matrix metalloproteinase-1, alanine aminotransferase, aspartate platelet count. FIB-4 index also calculated....

Abstract The aim of this study was to assess the relationship between HLA Class II alleles in two groups patients Latvia: with cystic and alveolar echinococcosis. included 37 from Rīga East Clinical University Hospital echinococcosis (29 eight echinococcosis) 100 healthy control persons without allele genotyping performed using Real-time polymerase chain reaction–sequence specific primer (RT-PCR-SSP). odds ratios (OR), 95% confidence intervals (95% CI), were calculated statistical analysis...

Abstract In this study, we sought to identify human leukocyte antigen (HLA) DRB1 alleles that might be associated with Lyme borreliosis in Latvian patients. Case patients and control subjects were similar age, sex, ethnic heritage differed only the presence of Borrelia burgdorferi infection. The frequency HLA-DRB1*07 (OR 3.52; p = 0.001), HLA-DRB1*15 3.02; 0.001) HLA-DRB1 *17 (03) 2.63; significantly increased disease compared groups. -DRB1*11(OR 0.37; 0.005) -DRB1*13 0.34; 0.002) was...

Optimization of training and minimization injuries are topical for the physical performance military personnel. Physical psycho-emotional load, fatigue, sleep deprivation, dietary limits can lead to development oxidative stress (OS) in specific training. This study investigated markers OS muscle damage cadets after a 10-day-long intensive course one-month-long recovery. The sample included 42 (2 females 40 males) aged from 22 till 34. Myoglobin, catalase activity (CAT), superoxide dismutase...

Background: Undeniably, sepsis is still a profoundly damaging and life-threatening condition for many individuals. The aim of this research was to investigate the genetically determined predisposition developed by analysis distribution human leukocyte antigen HLA class II genes. Methods & Materials: This single-center study at Pauls Stradins Clinical University Hospital in Latvia. group consisted patients (n = □62) who had were enrolled during 8-month period. immunogenetic part done 62...

Sepsis is a condition with high mortality rates and its diagnosis remains challenge. Therefore, new diagnostic biomarker or combinations of biomarkers for sepsis should be developed. In total, 80 patients who were suspected having followed up 7days. Quick Sepsis-related Organ Failure Assessment (qSOFA) scores calculated day 1 7; four (intercellular adhesion molecule-1, macrophage migration inhibitory factor, plasminogen activator inhibitor-1 soluble Fas ligand) results evaluated the same. 7...

This study aimed to detect the expression level of ORAI1 and STIM1 genes in blood patients with bilateral pulmonary tuberculosis (TB) comparison control group. Both encode proteins providing store-operated Ca2+ entry (SOCE) into cells, including immune activate transcriptional factors for producing cytokines inflammation-restricting proteins. The included 45 confirmed TB, aged 20 86, 35 volunteers, from 21 73, without active TB infection. was performed by real-time quantitative reverse...

<h3>Background</h3> Juvenile idiopathic arthritis (JIA) is group of heterogenous diseases [1]. Inflammatory cytokines, like Iiterleukin 6 (IL-6), IL-1 and tumor necrosis factor alpha (TNF-a) their regulatory gene polymorphisms can affect cytokine production, inflammatory processes disease outcome [2-4]. <h3>Objectives</h3> To determine the association <i>IL6</i>, <i>IL1A</i> <i>TNFA</i> SNPs with JIA. <h3>Methods</h3> All 107 JIA patients (28 seronegative 12 seropositive polyarthritis, 23...

Abstract The aim of this research was to investigate the role IFN-γ in interaction between IL-10, IL-18, IL-1b, CD4 cell counts and HIV-1 RNA viral load development patients co-infected with Mycobacterium tuberculosis (TB). study conducted by Rīga East Clinical University Hospital data from register, collaboration RSU Joint Laboratory Immunology Immunogenetics. 200 infected 184 TB co-infection divided four groups were included study. IFN-γ, IL-1b levels measured serum commercially...

Aim of Study: To identify the factors molecular genetic risks during development infection in HIV, based on TNFα cytokine gene polymorphism combination with HLA DRB1/DQA1/DQB1 genes, as well to analyse their possible association progress disease. 185 HIV infected patients and 173 individuals control group have been analysed. The DNA was extracted from peripheral blood, by using QiagenQIAamp kit reagents. quality quantity checked Qubit ® fluorometer typing for DRB1/DQB1/DQA1* performed RT-PCR...