This study evaluated the effects of different levels ultrasonic power (200, 400, 600 W) and treatment time (0, 10, 15 30 min) on structure, emulsification characteristics, in vitro digestibility chickpea protein isolate (CPI). The changes surface hydrophobicity CPI indicated that ultrasound exposed more hydrophobic amino acid residues. analysis sulfhydryl content zeta potential showed caused disulfide bond to be opened, releasing negatively charged groups, solution was stable. In addition,...

This study evaluated the effects of high intensity ultrasonication (HIU, 100, 150, 200, and 250 W) treatment time (0, 3, 6, 9, 12 min) on structure emulsification properties myofibrillar proteins (MPs) from Coregonus peled. These investigations were conducted using an ultrasonic generator at a frequency 20 kHz (ultrasonic probe). Analysis carbonyl content total number sulfhydryl groups showed that HIU significantly improved oxidative modification MPs (P < 0.05). SDS-PAGE profiling...

Pickering emulsions were prepared by stabilizing thymus vulgaris essential oil (TEVO) with cellulose nanocrystals (CNCs), which formed composite films loading the into modified tapioca/polyvinyl alcohol (PVA)-based films. The results showed that 1.0 % CNCs-15 TEVO emulsion had optimal stability and smaller particle size. increased thickness of film in form solid material additions (thickness, 0.062-0.099 mm), opacity given laminating film's superior UV-blocking ability compared to blank...

Allicin, as a natural antibacterial active substance from plants, has great medical and health care value. However, due to its poor stability, application in the field of food medicine is limited. So, this paper, allicin–zein–sodium caseinate composite nanoparticles (zein–Ali–SC) were prepared by antisolvent precipitation electrostatic deposition. Through analysis particle size, ζ-potential, encapsulation efficiency (EE), loading rate (LC) microstructure, optimum preparation conditions for...

As the most valuable protein in sperm of testes tissues Coregonus peled, peled protamine (CPP) had a natural antibacterial and antiseptic effect, but its physicochemical properties functions are easily affected by ultrasound-assisted extraction process. In this study, CPP was used to investigate effects different ultrasonic intensities (0, 3.03, 6.07, 9.10, 12.13, 15.16 W/cm2) on structural functional CPP. The results showed that at moderate intensity (9.10 W/cm2), successful, as it...

In order to develop low-salt and healthy surimi products, we limited the amount of NaCl 0.5 g/100 g in this work studied effect CaCl2 (0, 0.5, 1.0, 1.5, 2.0 g) on 3D printing quality gel. The results rheology showed that gel with 1.5 added could squeeze smoothly from nozzle had good self-support stability. chemical structure, interaction, water distribution, microstructure adding enhance water-holding capacity mechanical strength (the strength, hardness, springiness, etc.) by forming an...

Protamine is a promising natural preservative protein, and the extraction process can affect physicochemical properties function of protein. In this study, ultrasound-assisted (UA) acid was used to extract Coregonus peled protamine (CPP). Changes in extraction, functional CPP with ultrasound time (10, 20, 30, 40 50 min) were investigated. The Peleg model describe kinetics. results showed that 30 min UA treatment resulted highest protein yield (6.81%) (P ≤ 0.05). addition, secondary tertiary...

Traditional chemotherapy drugs have definite antitumor mechanisms and good therapeutic efficacy; however, their poor water solubility, serious side effects drug resistance limit clinical application. To the best of our knowledge, present study reported for first time in vivo vitro anticancer procyanidin B1 (PCB1), a compound that is isolated from natural sources such as grape seeds, apples, peanut skin cranberries. Cell Counting Kit-8 assay showed PCB1 effectively decreased number viable...

The objective of the study was to research effect protein oxidation on biochemical properties Coregonus peled muscle proteins. Myofibrillar proteins (MP) prepared from C. back oxidized using a hydroxyl radical-generating system (HRGS: 0.1 mM FeCl3 , ascorbic acid (Asc) and 1-20 H2 O2 ). In HRGS oxidizing system, carbonyls, dityrosine content, surface hydrophobicity MP (p < 0.05) increased with increasing concentration time, while total sulfhydryl, free amino groups Ca-ATPase activity...

Abstract Tenderness affects mutton quality and price, the degradation of myofibrillar protein (MP) is critical to improve tenderness. We investigated oxidative modification MP by hydroxyl radicals (OH) effects this on proteolysis µ‐calpain. As H 2 O concentrations increased, carbonyl dityrosine contents surface hydrophobicity all display an increasing trend, whereas total sulfhydryl intrinsic fluorescence intensity declines significantly. SDS‐PAGE electrophoresis indicates that disulfide...

In this study, the quality (sensory evaluation, microbial enumerate, color, tvb-n (total volatile basic nitrogen), tca-soluble peptide (trichloroacetic acid-soluble peptide), muscle glucose, lactate, total sugar, Bas (Biogenic amines), VOCs (volatile organic compounds) and dynamic structure in samples stored at 4 °C were evaluated, relationship between diversity of microorganisms was also discussed. It determined by sensory evaluation that shelf life around 8 days. Protein sugar detected...

Abstract The aim of this study is to identify the protein indicator freshness Coregonus peled using two‐dimensional gel electrophoresis (2‐DE) and matrix‐assisted laser desorption ionization‐time‐of‐flight/time‐of‐flight mass spectrometry (MALDI‐TOF/TOF MS) methods. Samples were obtained prior (control group) 2, 4, 6, 8 days after refrigerated storage for quality proteomics analysis. Three proteins found have significant differential abundance in sample groups during storage, including l...

Abstract The aim of this study was to evaluate the effect µ‐calpain oxidation on Coregonus peled myofibrillar protein degradation. In present study, a hydroxyl radical system selected investigate oxidative modification activity and its degradation C. protein. When subjected oxidation, carbonyl content significantly increased with increasing levels, promoted activity. Incubation oxidized resulted in enhanced myosin heavy chains, actin, troponin T, but desmin at higher levels slightly...

In this study, the effects of microwave power, vacuum degree and load weight on moisture content seedless white grapes (Vitis vinifera L.) during drying were investigated. The result found that drying, effective diffusivity (Deff) ranged between 1.0232 × 10−9 4.6354 m2/s. Deff values increased with increase in power decrease weight, played a relatively minor role. kinetics ratio time established, process following Page model, as reflected by fitting model-predicted measured data. model was...

The effects of changes in the malondialdehyde (MDA) content on quality fish during low-temperature storage period are unclear. Therefore, MDA Coregonus peled and protein were investigated following under refrigeration (4 °C) super chilling (-3 for 15 days (d). results showed that continued to increase highest was produced at 1.42 mg/kg refrigeration. fillet pH, drip loss, texture (hardness elasticity), myofibril fragmentation index deteriorated significantly period. Increased oxidation...

In the study, Coregonus peled protamine (CPP) prepared by ultrasound-assisted sulfuric acid extraction anion-exchange method was used to prepare and characterize CPP, physicochemical functional properties of CPP this were analyzed. The successfully (ultrasonic power density 9.10 W/cm2, ultrasonic time 30 min, temperature number times 3, concentration 0.15 M, liquid/feed ratio 3:1), results copper sulfate showed negative, Sakaguchi's reaction positive. that a protein containing only one kind...

The effects of the degree oxidation (0.01 mmol/L FeCl3, 0.1 ascorbic acid, 1 - 20 H2O2) principally induced by Fenton systems on structural and functional properties Coregonus peled myofibrillar proteins (MP) was investigated. When levels MP increased, their carbonyl groups increased but sulfhydryl decreased (p &lt; 0.05). SDS-PAGE analysis revealed more cross-linking protein polymerisation in oxidised MP. FTIR suggested that conformational changes results indicated moderate (5 10 improved...