A5069440310- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Antimicrobial Peptides and Activities
- Food Allergy and Anaphylaxis Research
- Complement system in diseases
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Calcium signaling and nucleotide metabolism
- Allergic Rhinitis and Sensitization
- Mast cells and histamine
- CAR-T cell therapy research
- Contact Dermatitis and Allergies
- Toxoplasma gondii Research Studies
- Immune Response and Inflammation
Institut Pasteur
2024-2025
Université Paris Cité
2024-2025
Inserm
2024-2025
Constructor University
2013-2021
Universitätsmedizin Göttingen
2020
MHC class I molecules bind only those peptides with high affinity that conform to stringent length and sequence requirements. We have now investigated which can aid the in vitro folding of molecules, we find dipeptide glycyl-leucine efficiently supports HLA-A*02:01 H-2K b into a peptide-receptive conformation rapidly binds high-affinity peptides. Treatment cells induces accumulation at surface cells. Other dipeptides hydrophobic second amino acid show similar enhancement effects. Our data...
Significance We have shown previously that dipeptides can support efficient in vitro folding of the MHC class I molecules. Here, we describe discovery catalyze dissociation low- and high-affinity peptides from their replacement for exogenous interest, on both recombinant cell surface HLA-A*02:01, HLA-B*27:05, H-2K b Understanding peptide exchange will help us understand optimization live cells. demonstrate peptide-exchange technology be used to produce epitope-specific tetramers much faster...
Understanding the phenotypic and transcriptional signature of immunoglobulin E (IgE)–producing cells is fundamental to plasma cell (PC) biology development therapeutic interventions for allergy. Here, using a mouse model intranasal house dust mite (HDM) exposure, we showed that short-lived IgE PCs emerge in lung draining lymph nodes (dLNs) during early exposure (<3 weeks) long-lived accumulate bone marrow (BM) with prolonged (>7 weeks). had distinct surface gene expression profiles...
Major histocompatibility complex (MHC) class I molecules (proteins) bind peptides of eight to ten amino acids present them at the cell surface cytotoxic T cells. The binding groove binds peptide via hydrogen bonds with termini and diverse interactions anchor residue side chains peptide. To elucidate which these is most important for thermodynamic kinetic stability peptide-bound state, we have combined molecular dynamics simulations experimental approaches in an investigation conformational...
The murine cytomegalovirus immunoevasin m152/gp40 binds major histocompatibility complex (MHC) class I molecules and retains them in the early secretory pathway by a previously unknown mechanism, preventing antigen presentation to CD8+ T cells. We show that retention of gp40 itself depends on lumenal linker sequence gp40. With unbiased co-immunoprecipitation mass spectrometry, we find that, through this linker, TMED10/Tmp21/p24δ1, member p24 family endoplasmic reticulum (ER)/Golgi...
ABSTRACT In the presence of murine cytomegalovirus (mCMV) gp40 (m152) protein, major histocompatibility complex (MHC) class I molecules do not reach cell surface but are retained in an early compartment secretory pathway. We find that does impair folding or high-affinity peptide binding binds to them, leading their retention endoplasmic reticulum (ER), ER-Golgi intermediate (ERGIC) and cis-Golgi, most likely by retrieval from cis-Golgi ER. identify a sequence is required for both its own...
We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture specific forms MHC proteins that allow location- analysis by fluorescence microscopy. The is in cis under controlled conditions define involved protein conformations. Our results show homotypic occur exclusively between free heavy...
ABSTRACT NKG2D (also known as KLRK1) is a crucial natural killer (NK) cell-activating receptor, and the murine cytomegalovirus (MCMV) employs multiple immunoevasins to avoid NKG2D-mediated activation. One of MCMV immunoevasins, gp40 (m152), downregulates cell surface ligand RAE-1γ Raet1c) thus limiting NK This study establishes molecular mechanism by which retains in secretory pathway. Using flow cytometry pulse-chase analysis, we demonstrate that early pathway, this effect depends on...
Abstract MHC (Major Histocompatibility Complex) class I molecules play a central role in the mammalian antiviral immune response by presenting viral proteome at cell surface for recognition cytotoxic T lymphocytes. The folding of recombinant from denatured bacterial inclusion bodies and their assembly with light chain beta-2 microglobulin largely depend on specific high-affinity peptides usually eight or nine amino acids. To find minimum peptide requirement stabilization molecules, we have...
Abstract NKG2D is a crucial Natural Killer (NK) cell activating receptor, and the murine cytomegalovirus (MCMV) employs multiple immunoevasins in order to avoid NKG2D-mediated activation. One of MCMV immunoevasins, gp40 ( m152 ), downregulates surface ligand, RAE-1γ, thus limiting NK This study establishes molecular mechanism by which retains RAE-1γ secretory pathway. Using flow cytometry pulse chase analysis, we demonstrate that early pathway, this effect depends on binding host protein,...