A5033208114- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Vibrio bacteria research studies
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Viral-associated cancers and disorders
- Gene Regulatory Network Analysis
- Liver Disease Diagnosis and Treatment
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- Hepatitis C virus research
Université Claude Bernard Lyon 1
2006-2024
École Normale Supérieure de Lyon
2003-2024
Centre International de Recherche en Infectiologie
2015-2019
Inserm
2003-2015
Centre National de la Recherche Scientifique
2015
The current outbreak of Ebola virus (EBOV) in West Africa has claimed the lives more than 15,000 people and highlights an urgent need for therapeutics capable preventing replication. In this study we screened known nucleoside analogues their ability to interfere with EBOV Among them, cytidine analogue β-d-N4-hydroxycytidine (NHC) demonstrated potent inhibitory activities against replication spread at non-cytotoxic concentrations. Thus, NHC constitutes interesting candidate development a...
VP30 is a phosphoprotein essential for the initiation of Ebola virus transcription. In this work, we have studied effect mutations in phosphorylation sites on ebolavirus replication cycle by using reverse genetics system. We demonstrate that involved reinitiation gene transcription and activity affected at sites.
Ebola virus (EBOV) transcription is dependent on the phosphoprotein VP30, a component of viral nucleocapsid. VP30 phosphorylated at 2 serine residue clusters located N-terminal part protein. In this report, we have investigated role phosphorylation in EBOV replication using reverse genetics approach. effect, recombinant EBOVs with substituted either by nonphosphorylatable alanines or phosphorylation-mimicking aspartates were generated and characterized. We show that comparison to wild-type...
Transient expression of Ebola virus (EBOV) glycoprotein GP causes downregulation surface proteins, cell rounding and detachment, a phenomenon believed to play central role in the pathogenicity virus. In this study, evidence that moderate does not result such morphological changes was provided. It shown continuously produced 293T cells from Kunjin replicon correctly processed transported plasma membrane without affecting β 1 α 5 integrins major histocompatibility complex I molecules. The...
The human parvovirus Adeno-Associated Virus (AAV) type 2 can only replicate in cells co-infected with a helper virus, such as Adenovirus or Herpes Simplex 1 (HSV-1); whereas, the absence of it establishes latent infection. Previous studies demonstrated that ternary HSV-1 helicase/primase (HP) complex (UL5/8/52) and single-stranded DNA-Binding Protein (ICP8) were sufficient to induce AAV-2 replication transfected cells. We independently showed that, context infection, ICP0 protein was able...
ABSTRACT Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transcriptional activator involved in the immortalization of B lymphocytes by virus. EBNA2 targeted to promoters its responsive genes, via interaction with cellular DNA-binding proteins. Using chromatin immunoprecipitation assays, we show for first time conditional recruitment on two specific viral vivo and demonstrate correlation between this local change acetylation histones H3 H4, which promoter dependent.
ABSTRACT Adeno-associated virus (AAV) is a human parvovirus that replicates only in cells coinfected with helper virus, such as adenovirus or herpes simplex type 1 (HSV-1). We previously showed nine HSV-1 factors are able to support AAV rep gene expression and genome replication. To elucidate the strategy of replication presence HSV-1, we undertook proteomic analysis cellular associated Rep proteins thus potentially recruited within compartments (AAV RCs). This study resulted identification...
The Ebola virus matrix protein VP40 plays an essential role in assembly and budding. In this study we reveal that transient expression results the release into culture medium of substantial amounts soluble monomeric addition to virus-like particles containing oligomeric form as previously described. We show secretion is endoplasmic reticulum/Golgi-independent not associated with cell death. Soluble was observed during infection cells also found serum virus-infected animals albeit lower...
Les progrès récents de la biologie synthétique ont ouvert voie à nouvelles thérapies fondées sur des cellules rendues aptes produire manière autonome substrats afin traiter maladies chroniques. Ces modifiées intègrent un ensemble gènes fonctionnant en circuit boucle fermée, qui permettent délivrer effecteur thérapeutique réponse signal pathologique déterminé. Bien que prometteuses chez souris, ces font face obstacles cliniques liés leur sûreté et implémentation in vivo . dernières...
La frequence des epidemies de fievres hemorragiques a virus Ebola ou Marburg constatee en Afrique Centrale est augmentation depuis une dizaine d’annees. Une vigilance accrue permet detecter tres precocement la plupart ces epidemies, mais quarantaine stricte reste le seul moyen lutter contre virus : effet, il n’existe ce jour ni vaccin, traitement utilisable chez l’homme, meme si recherche progresse. Cet article fait point sur deux resultats recents. Le premier decouverte du mecanisme par...