A5058984388- Advanced X-ray and CT Imaging
- Medical Imaging Techniques and Applications
- Radiation Dose and Imaging
- Photoacoustic and Ultrasonic Imaging
- Radiomics and Machine Learning in Medical Imaging
- Cardiac Imaging and Diagnostics
- Optical Imaging and Spectroscopy Techniques
- Ultrasound and Hyperthermia Applications
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced MRI Techniques and Applications
Duke University
2022-2024
Duke University Hospital
2022-2024
Duke Medical Center
2022-2024
Photon-counting CT (PCCT) is powerful for spectral imaging and material decomposition but produces noisy weighted filtered backprojection (wFBP) reconstructions. Although iterative reconstruction effectively denoises these images, it requires extensive computation time. To overcome this limitation, we propose a deep learning (DL) model, UnetU, which quickly estimates from wFBP. Utilizing 2D U-net convolutional neural network (CNN) with custom loss function transformation of wFBP, UnetU...
3D bioprinting is revolutionizing the fields of personalized and precision medicine by enabling manufacturing bioartificial implants that recapitulate structural functional characteristics native tissues. However, lack quantitative noninvasive techniques to longitudinally track function has hampered clinical applications bioprinted scaffolds. In this study, multimaterial bioprinting, engineered nanoparticles (NPs), spectral photon-counting computed tomography (PCCT) technologies are...
Objective.Photon-counting CT (PCCT) has better dose efficiency and spectral resolution than energy-integrating CT, which is advantageous for material decomposition. Unfortunately, the accuracy of PCCT-based decomposition limited due to distortions in photon-counting detector (PCD).Approach.In this work, we demonstrate a deep learning (DL) approach that compensates PCD improves by using maps provided high-dose multi-energy-integrating (EID) data as training labels. We use 3D U-net...
The purpose of this study was to investigate if radiomic analysis based on spectral micro-CT with nanoparticle contrast-enhancement can differentiate tumors lymphocyte burden. High mutational load transplant soft tissue sarcomas were initiated in Rag2+/− and Rag2−/− mice model varying Mice received radiation therapy (20 Gy) the tumor-bearing hind limb injected a liposomal iodinated contrast agent. Five days later, animals underwent conventional imaging using an energy integrating detector...
Abstract Objective. To evaluate the performance of two photon-counting (PC) detectors based on different detector materials, gallium arsenide (GaAs) and cadmium telluride (CdTe), for PC micro-CT imaging phantoms with multiple contrast materials. Another objective is to determine if combining these in same system can offer higher spectral significant artifact reduction compared a single system. Approach . We have constructed dual-detector, equipped PCDs materials: (CdTe). demonstrate up 5...
Background Cardiovascular disease (CVD) is associated with the apolipoprotein E (APOE) gene and lipid metabolism. This study aimed to develop an imaging-based pipeline comprehensively assess cardiac structure function in mouse models expressing different APOE genotypes using photon-counting computed tomography (PCCT). Methods 123 mice grouped based on genotype (APOE2, APOE3, APOE4, knockout (KO)), gender, human NOS2 factor, diet (control or high fat) were used this study. The included PCCT...
Photon Counting Detectors (PCD) have emerged as a transformative technology in CT and micro-CT imaging, offering enhanced contrast resolution quantitative material separation single scan, notable advancement from traditional energy-integrating detectors. The unique properties of bismuth tungstate (Bi<sub>2</sub>WO<sub>6</sub>) nanoparticles (NPs), hold promise many applications, including contrast-enhanced imaging photothermal therapy, especially addressing tumor hypoxia challenges. However,...
Spectral micro-CT shows great potential to provide accurate material composition by utilizing the energy dependence of x-ray attenuation in different materials. This is especially well-suited for pre-clinical imaging using nanoparticle-based contrast agents situations where quantitative decomposition helps probe processes which are otherwise limited poor soft tissue contrast. Our group has developed multiple generations prototype PCCT systems and applied them cancer cardiac studies...
In preclinical studies, micro-CT is frequently employed to yield valuable anatomical insights. However, there has been an increasing need for in extracting functional measurements such as with perfusion imaging. Perfusion imaging plays a crucial role understanding and quantifying tissue vascular properties. This paper focuses on our development of micro-photon counting (PC)CT demonstrates quantification metrics controlled fluid flow phantom experiment. For this study, we utilized novel...
Photon-counting detectors (PCDs) are advantageous for spectral CT imaging and material decomposition because they simultaneously acquire projections at multiple energies using energy thresholds. Unfortunately, the PCD produces noisy weighted filtered backprojection (wFBP) reconstructions due to diminished photon counts in high-energy bins. Iterative reconstruction generates high quality images, but requires long computation time, especially 5D (3D + time) vivo cardiac imaging. Our recent...
Recently, we have released the first open-source version of our Multi-Channel CT Reconstruction (MCR) Toolkit (https://gitlab.oit.duke.edu/dpc18/mcr-toolkit-public). The initial release represents 10 years development and provides a complete set GPU-accelerated tools for solving multi-channel (multi-energy, time-resolved) X-ray reconstruction problems with support both analytical iterative in common preclinical clinical geometries. This (v1.0) relies on MATLAB its MEX interface orchestrating...
This paper presents a deep learning approach to automated segmentation of cardiac structures in 5D (3D + Time Energy) Photon-Counting micro-CT (PCCT) imaging sets. We have acquired, reconstructed, and fully segmented preclinical dataset micro-PCCT scans APOE mouse models. These genotypes serve as models varying degrees risk Alzheimer's disease cardiovascular disease. The user-guided segmentations served the training data for 3D UNet model capable segmenting four primary chambers, aorta,...
Brain region segmentation and morphometry in mouse models of Alzheimer's Disease (AD) risk allow us to understand how various factors affect the brain. Photon-Counting Detector (PCD) micro-CT can provide faster brain imaging than MRI superior contrast spatial resolution Energy-Integrating (EID) micro-CT. This paper demonstrates a PCD based approach for imaging, segmentation, morphometry. We extracted stained brains 26 mice from three genotypes (APOE22HN, APOE33HN, APOE44HN). scanned these...
This study investigated the application of VivoVist™, a high-contrast micro-CT contrast agent, in spectral Photon-Counting (PC) imaging mouse models. With long blood half-life, superior concentration, and reduced toxicity VivoVist, composed barium (Ba)-based nanoparticles, offers cost-effective solution for enhancing Computed Tomography (CT) imaging. To evaluate its efficacy, we employed an in-house developed with photon-counting detector. VivoVist was administered through retro-orbital...
Background Brain region segmentation and morphometry in humanized apolipoprotein E ( APOE ) mouse models with a human NOS2 background HN contribute to Alzheimer’s disease (AD) research by demonstrating how various risk factors affect the brain. Photon-counting detector (PCD) micro-CT provides faster scan times than MRI, superior contrast spatial resolution energy-integrating (EID) micro-CT. This paper presents pipeline for brain imaging, segmentation, from PCD Methods We used brains of 26...
Abstract Objective. This study introduces a novel desktop micro-CT scanner designed for dynamic perfusion imaging in mice, aimed at enhancing preclinical capabilities with high resolution and low radiation doses. Approach. The system features custom-built rotating table capable of both circular helical scans, enabled by small-bore slip ring continuous rotation. Images were reconstructed temporal 3.125 s an isotropic voxel size 65 µ m, potential higher scanning. system’s static performance...
Abstract Objective. &#xD;Photon-counting detectors (PCDs) for CT imaging use energy thresholds to simultaneously acquire projections at multiple energies, making them suitable spectral and material decomposition. Unfortunately, setting results in noisy analytical reconstructions due low photon counts high-energy bins. Iterative reconstruction provides high quality photon-counting (PCCT) images but requires enormous computation time 5D (3D + time) vivo cardiac imaging....
Developing novel contrast agents for multi-energy photon-counting (PC)CT will require a clear translation pathway from preclinical validation to clinical applications. To begin this development, we have used PCCT scanner (Siemens NAEOTOM Alpha) study the spectral separation of few elements (Iodine, I; Gadolinium, Gd; Hafnium, Hf; Tantalum, Ta; Bismuth, Bi; Calcium, Ca) with currently available scanning protocols (fixed: 120 kVp, 20 and 65 keV thresholds). We also explored capabilities image...
Photon-counting detector (PCD) CT promises to improve routine imaging applications with higher spatial resolution, lower levels of noise at fixed dose, and improved image contrast while providing spectral information every scan. We propose demonstrate a novel application PCD in preclinical model head neck squamous cell carcinoma: perfusion cancer. To handle the high dimensionality our data set (3D volumes 12 time points times 4 energy thresholds), we update previously proposed multi-channel...
The purpose of this study was to investigate if radiomic analysis based on spectral micro-CT with nanoparticle contrastenhancement can differentiate tumors tumor-infiltrating lymphocyte (TIL) burden. High mutational load transplant soft tissue sarcomas were initiated in Rag2+/- and Rag2-/- mice model varying TIL Mice received radiation therapy (20 Gy) the tumor-bearing hind limb injected a liposomal iodinated contrast agent. Five days later, animals underwent conventional imaging using an...