A5050476826- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pluripotent Stem Cells Research
- Additive Manufacturing and 3D Printing Technologies
- Congenital Heart Disease Studies
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neural Engineering
- Bone Tissue Engineering Materials
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Tracheal and airway disorders
- Nanoplatforms for cancer theranostics
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Anatomy and Medical Technology
- Cellular Mechanics and Interactions
- Photoacoustic and Ultrasonic Imaging
- Medical Imaging Techniques and Applications
- Head and Neck Surgical Oncology
- Cleft Lip and Palate Research
- Oral microbiology and periodontitis research
- Williams Syndrome Research
- Advanced X-ray and CT Imaging
- Silk-based biomaterials and applications
Georgia Institute of Technology
2018-2025
Emory University
2018-2025
Children's Healthcare of Atlanta
2023-2025
The Wallace H. Coulter Department of Biomedical Engineering
2019-2024
Emory and Henry College
2021
Broad Institute
2018-2021
Harvard University
2018
Massachusetts Institute of Technology
2018
SUNY Polytechnic Institute
2015-2016
Three-dimensional (3D) bioprinting of hydrogel-based constructs at adequate consistency and reproducibility can be obtained through a compromise between the hydrogel's inherent instability printing fidelity. There is an increasing demand to develop modalities that enable high-fidelity fabrication 3D hydrogel structures closely correspond envisioned design. In this work, we performed systematic, in-depth characterization optimization embedded create gelatin-methacryloyl (gelMA) with highly...
Neuroblastoma (NB) is the most common extracranial tumor in children resulting substantial morbidity and mortality. A deeper understanding of NB microenvironment (TME) remains an area active research but there a lack reliable biomimetic experimental models. This study utilizes 3D bioprinting approach, combination with spheroids, to create vitro vascular model for exploring function within endothelialized microenvironment. gelatin methacryloyl (gelMA) bioink used multi-channel cubic analogues...
Abstract The ability to promote three‐dimensional (3D) self‐organization of induced pluripotent stem cells into complex tissue structures called organoids presents new opportunities for the field developmental biology. Brain have been used investigate principles neurodevelopment and neuropsychiatric disorders serve as a drug screening discovery platform. However, brain organoid cultures are currently limited by lacking precisely control their extracellular environment. Here, this work...
Abstract Vascular cell overgrowth and lumen size reduction in pulmonary vein stenosis (PVS) can result elevated PV pressure, hypertension, cardiac failure, death. Administration of chemotherapies such as rapamycin have shown promise by inhibiting the vascular proliferation; yet clinical success is limited due to complications restenosis off‐target effects. The lack vitro models recapitulate complex pathophysiology PVS has hindered identification disease mechanisms therapies. This study...
Current strategies for regeneration of large bone fractures yield limited clinical success mainly due to poor integration and healing. Multidisciplinary approaches in design development functional tissue engineered scaffolds are required overcome these translational challenges. Here, a new generation hyperelastic (HB) implants, loaded with superparamagnetic iron oxide nanoparticles (SPIONs), 3D bioprinted their regenerative effect on non-healing is studied. Scaffolds the geometry that...
Abstract Adhesive tissue engineering scaffolds (ATESs) have emerged as an innovative alternative means, replacing sutures and bioglues, to secure the implants onto target tissues. Relying on their intrinsic adhesion characteristics, ATES systems enable minimally invasive delivery of various scaffolds. This study investigates development first class 3D bioprinted constructs using functionalized hydrogel bioinks. Two strategies, in situ printing adherend versus then transferring surface, are...
The oral microbiome is a complex community that matures with dental development and recognized as risk factor for systemic disease. Despite the cavity having substantial microbial burden, healing of superficial wounds occurs quickly little scarring. By contrast, creation an oro-nasal fistula (ONF), often occurring after surgery to correct cleft palate, significant wound challenge. Methods: In this study, we characterized changes in mice following freshly inflicted palate results open...
Abstract The heart is the first organ to develop in human embryo through a series of complex chronological processes, many which critically rely on interplay between cells and dynamic microenvironment. Tight spatiotemporal regulation these interactions key development diseases. Due suboptimal experimental models, however, little known about role microenvironmental cues development. This study investigates use 3D bioprinting perfusion bioreactor technologies create bioartificial constructs...
Abstract The human genome with all its ethnic variations contributes to differences in development, aging, disease, repair and response medical treatments is an exciting area of research clinical study. availability well-characterized ethnically diverse stem cell lines limited has not kept pace other advances research. Here we derived xenofree diverse-human induced pluripotent (ED-iPSC) from fibroblasts obtained individuals African American, Hispanic-Latino, Asian Caucasian origin have...
Background Tetralogy of Fallot with major aortopulmonary collateral arteries is a heterogeneous form pulmonary artery (PA) stenosis that requires multiple forms intervention. We present patient-specific in vitro platform capable sustained flow can be used to train proceduralists and surgical teams current interventions, as well developing novel therapeutic approaches treat various vascular anomalies. Our objective develop an model PA based on patient data phantom cardiovascular disease...
Biomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, use materials bioprinting-based approaches fabrication has not been examined. This study introduces a new generation bacteriostatic gelatin methacryloyl (GelMA)-based bioinks, incorporated with varying doses...
3D bioprinting is revolutionizing the fields of personalized and precision medicine by enabling manufacturing bioartificial implants that recapitulate structural functional characteristics native tissues. However, lack quantitative noninvasive techniques to longitudinally track function has hampered clinical applications bioprinted scaffolds. In this study, multimaterial bioprinting, engineered nanoparticles (NPs), spectral photon-counting computed tomography (PCCT) technologies are...
Vascular atresia are often treated via transcatheter recanalization or surgical vascular anastomosis due to congenital malformations coronary occlusions. The cellular response is, however, largely unknown and current techniques rely on restoration rather than optimization of flow into the atretic arteries. An improved understanding post may result in reduced restenosis. Here, an vitro platform is used model pulmonary arteries (PAs) for procedural planning reduce Bifurcated PAs bioprinted...
The realization of personalized medicine through human induced pluripotent stem cell (iPSC) technology can be advanced by transcriptomics, epigenomics, and bioinformatics that inform on genetic pathways directing tissue development function. When possible, population diversity should included in new studies as resources become available. Previously we derived replicate iPSC lines African American, Hispanic-Latino Asian self-designated ethnically diverse (ED) origins with normal karyotype,...
Abstract BACKGROUND COVID-19 poses a risk to the endoscopic skull base surgeon. Significant efforts improving safety have been employed, including use of personal protective equipment, preoperative testing, and recently modified surgical mask barrier. OBJECTIVE To reduce risks pathogen transmission during surgery. METHODS This study was exempt from Institutional Review Board approval. Our utilizes 3-dimensional (3D)-printed with an anterior aperture fitted glove ports designed allow for...
Abstract Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization the spatial-temporal evolution cell state in 3D culture subsequent 2D dissociated based on protein expression levels localizations is essential to understanding differentiation, disease phenotypes, sample-to-sample variability. Here, we apply PR obe-based I...
Photocrosslinked hydrogels, such as methacrylate‐modified gelatin (gelMA) and hyaluronic acid (HAMA), are widely utilized tissue engineering scaffolds and/or drug delivery vehicles, but lack a suitable means for noninvasive, longitudinal monitoring of surgical placement, biodegradation, release. Therefore, novel photopolymerizable X‐ray contrast agent, gold nanoparticles (AuMA NPs), is developed to enable covalent‐linking hydrogels (gelMA HAMA) in one‐step during photocrosslinking...
Introduction Primary pulmonary vein stenosis (PVS) is a rare congenital heart disease that proves to be clinical challenge due the rapidly progressive course and high rates of treatment complications. PVS intervention frequently faced with in-stent restenosis persistent progression despite initial venous recanalization balloon angioplasty or stenting. Alterations in wall shear stress (WSS) have been previously associated neointimal hyperplasia underlying progression. Thus, development...