A5101815447- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Silk-based biomaterials and applications
- Virus-based gene therapy research
- Protein purification and stability
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- thermodynamics and calorimetric analyses
- RNA Research and Splicing
- Protein Hydrolysis and Bioactive Peptides
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Antioxidant Activity and Oxidative Stress
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- Silkworms and Sericulture Research
- Enzyme Structure and Function
- Plant-Microbe Interactions and Immunity
- DNA and Nucleic Acid Chemistry
- Viral gastroenteritis research and epidemiology
Jiangsu University
2012-2025
Zhanjiang Experimental Station
2018
Cangzhou Central Hospital
2017
Peking University
2009
Zhejiang University
2005-2006
Kyoto University
1993
Research Article| March 01, 2010 Age, geochemistry, and tectonic implications of a late Paleozoic stitching pluton in the North Tian Shan suture zone, western China Bao-Fu Han; Han † 1Ministry Education, Key Laboratory Orogenic Belts Crustal Evolution, School Earth Space Sciences, Peking University, Beijing 100871, People's Republic †E-mail: bfhan@pku.edu.cn Search for other works by this author on: GSW Google Scholar Zhao-Jie Guo; Guo Zhi-Cheng Zhang; Zhang Lei Jia-Fu Chen; Chen Biao Song...
Some feed source plants will produce secondary metabolites such as cyanogenic glycosides during metabolism, which some poisonous nitrile compounds after hydrolysis and remain in plant tissues. The consumption of feed-source without proper treatment affect the health animals' bodies. Nitrilases can convert nitriles have been used industry green biocatalysts. However, due to their bottleneck problems, application agriculture is still facing challenges. Acid-resistant nitrilase preparations,...
A nuclear export signal (NES) is a cluster of hydrophobic amino acids that can maintain the dynamic shuttling target proteins between nucleus and cytoplasm. Bioinformatics analysis showed region 92PLLLHKFLLA in Bm65 very likely to be an NES may involved production infectious virions. In this study, we generated several mutations Bm65, which were further used generate recombinant viruses study their roles viral propagation. Subcellular revealed sequence was transport Bm65. Mutations could...
Abstract Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production by the highly expressed protein polyhedrin Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye verified colocalization aggresomal markers, GFP-250 γ−tubulin. Polyhedrin showed hallmark characteristics aggresomes: microtubule-dependent; they colocalized heat shock cognates/proteins 70-kDa family (HSC/HSP70s),...
Addressing the demand for efficient biological degradation of straw, this study employed rational design coupled with structural biology and enzyme engineering techniques to enhance catalytic activity cellobiose dehydrogenase (
Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue Autographa californica multiple NPV ORF81. The gene conserved among all baculoviruses and thus considered baculovirus core gene. transcript BmORF67 was detected at 18–72 h post‐infection (p.i.). Polyclonal antiserum raised to His‐BmORF67 fusion protein recognized in infected cell lysates from 24 72 p.i., suggesting that late not either budded viruses or occlusion‐derived virus. Immunofluoresence analysis...
Two quinolinium conjugates as G-quadruplex probes were presented. The binding properties and mechanism investigated using both experimental docking studies.
Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight 31.0 kDa. Bm60 its homologues have been identified 11 completely sequenced lepidopteran NPVs. The transcript was detected by RT-PCR at 18-72 h post-infection (p.i.), while corresponding protein could be 24-72 p.i. BmNPV-infected BmN cells Western blot analysis using a polyclonal antibody against...
Polyhedra can encapsulate other proteins and have potential applications as protein stabilizers. The extremely stable polyhedra matrix may provide a platform for future engineered micro-crystal devices. However, the composition of remains largely unknown. In this study, occlusion-derived virus (ODV)-removed BmNPV fraction was subjected to SDS-PAGE then an LC-ESI-MS/MS analysis using Thermo Scientific Q Exactive mass spectrometer. total, 28 host 91 viral were identified. components grouped...
Vacuolar protein sorting 4 (Vps4) not only functions with its positive regulator vacuolar 20-associated 1 (Vta1) in the multivesicular body (MVB) pathway but also participates alone MVB-unrelated cellular processes. However, physiological roles at organism level remain rarely explored. We previously identified their respective homologues Bombyx mori Vps4 (BmVps4) and BmVta1 from silkworm, a model for insect research. In this study, we performed fluorescence quantitative real-time PCR Western...
In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences similar structure to human adiponectin (AdipoR). Structural analysis translated cDNA suggested it encoded a membrane protein seven transmembrane domains. BmAdipoR was found be expressed in multiple tissues Malpighian tubules, fat body testis. BmNPV (Bombyx nucleopolyhedrovirus) bacmid system combined confocal microscopy...