A5030073721- Fungal and yeast genetics research
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Plant-Microbe Interactions and Immunity
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Fungal Biology and Applications
- Plant Reproductive Biology
- thermodynamics and calorimetric analyses
- Melanoma and MAPK Pathways
- Heat shock proteins research
- Advanced Proteomics Techniques and Applications
- Genomics and Chromatin Dynamics
- Polyamine Metabolism and Applications
- Protein Kinase Regulation and GTPase Signaling
- Advanced Fluorescence Microscopy Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Breast Cancer Therapies
- Calpain Protease Function and Regulation
- Postharvest Quality and Shelf Life Management
- Hippo pathway signaling and YAP/TAZ
- Gene Regulatory Network Analysis
- Evolution and Genetic Dynamics
- Wnt/β-catenin signaling in development and cancer
ETH Zurich
2012-2023
Institute for Biomedical Engineering
2020
Scripps Research Institute
2002-2012
École Polytechnique Fédérale de Lausanne
2007
Swiss Group For Clinical Cancer Research
2000
hCDC4, the gene that encodes F-box protein responsible for targeting cyclin E ubiquitin-mediated proteolysis, has been found to be mutated in a number of primary cancers and cancer-derived cell lines. We have observed functional inactivation hCDC4 does not necessarily correlate with elevated levels tumors. Here we show, however, mutation tumors correlates strongly loss cycle regulation E. Similarly, breast carcinoma-derived line exhibits deregulation E, but periodic expression is restored by...
Significance Cells are constantly exposed to a variety of mechanical cues, and respond by activating conserved signaling pathways that modulate their profiliation intracellular organization. For instance, cells in solid tumors experience sustained compression from the microenvironment, compressive stress is known trigger an invasive phenotype some cancer cells. However, despite its importance for health disease, specific mechanosensors downstream network mediating these physiological...
Journal Article An integrated image analysis platform to quantify signal transduction in single cells Get access Serge Pelet, Pelet ETH-Zurich, Institute of Biochemistry, Schafmattstr. 18, CH-8093 Zurich, Switzerland E-mail: serge.pelet@bc.biol.ethz.ch Search for other works by this author on: Oxford Academic Google Scholar Reinhard Dechant, Dechant Sung Sik Lee, Lee Frank van Drogen, Drogen Matthias Peter matthias.peter@bc.biol.ethz.ch Integrative Biology, Volume 4, Issue 10, October 2012,...
Cyclin E1, an activator of cyclin-dependent kinase 2 (Cdk2) that promotes replicative functions, is normally expressed periodically within the mammalian cell cycle, peaking at G1-S-phase transition. This periodicity achieved by E2F-dependent transcription in late G1 and early S phases ubiquitin-mediated proteolysis. The ubiquitin ligase targets phosphorylated cyclin E SCFFbw7 (also known as SCFCdc4), a member cullin ring (CRL) family. Fbw7, substrate adaptor subunit, three splice-variant...
Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mechanisms remain poorly understood. Here, we show that mechanical activates Pkc1, which prevents lysis of pheromone-treated cells inhibiting polarized growth. vitro Pkc1 phosphorylates conserved residues within RING-H2 domains scaffold proteins Far1 Ste5, are...
AbstractThe ubiquitin-mediated turnover of cyclin E is regulated by phosphorylation and the activity ubiquitin ligase SCFCdc4 (also known as SCFFbw7). In 293A cells, SCF complexes containing two different Cdc4 isoforms, α γ, are required for efficient ubiquitylation. Whereas SCFCdc4γ ubiquitylates directly, SCFCdc4α serves a cofactor Pin1-mediated prolyl isomerization phosphodegron, essential to potentiate current study, we show that requirement both Cdc4α γ general, except in cell lines...
Abstract Cellular decision making often builds on ultrasensitive MAPK pathways. The phosphorylation mechanism of MAP kinase has so far been described as either distributive or processive, with mechanisms generating ultrasensitivity in theoretical analyses. However, the vivo and its activation dynamics remain unclear. Here, we characterize regulation Hog1 Saccharomyces cerevisiae via topologically different ODE models, parameterized multimodal data. Interestingly, our best fitting model...
Green fluorescent proteins (GFPs) are widely used tools to visualize and study their intracellular distribution. One feature of working with GFP variants, photobleaching, has recently been combined an older technique known as fluorescence recovery after photobleaching (FRAP) protein kinetics in vivo. During fluorochromes get destroyed irreversibly by repeated excitation intensive light source. When the is applied a restricted area or structure, will be result active passive diffusion from...
Abstract Ste5 is a scaffold protein that controls the pheromone response of MAP‐kinase cascade in yeast cells. Upon stimulation, (through its RING‐H2 domain) interacts with β and γ subunits an activated heterodimeric G promotes activation cascade. With structural biophysical studies, we show domain exists as molten globule under native buffer conditions, extracts, even denaturing conditions containing urea (7 M ). Furthermore, it exhibits high thermal stability conditions. Binding to...
Abstract Ste5 is a scaffold protein that controls the pheromone response of MAP‐kinase cascade in yeast cells. Upon stimulation, (through its RING‐H2 domain) interacts with β and γ subunits an activated heterodimeric G promotes activation cascade. With structural biophysical studies, we show domain exists as molten globule under native buffer conditions, extracts, even denaturing conditions containing urea (7 M ). Furthermore, it exhibits high thermal stability conditions. Binding to...