A5051155102- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Insect symbiosis and bacterial influences
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Cancer Research and Treatments
- Telomeres, Telomerase, and Senescence
Novo Nordisk Foundation
2023
Technical University of Denmark
2023
University of Copenhagen
2020-2021
Vilnius University
2020
Abstract Oncogene-induced senescence provides a barrier against malignant transformation. However, it can also promote cancer through the secretion of plethora factors released by senescent cells, called associated secretory phenotype (SASP). We have previously shown that in proliferating nuclear lncRNA MIR31HG inhibits p16/CDKN2A expression interaction with polycomb repressor complexes and during BRAF-induced senescence, is overexpressed translocates to cytoplasm. Here, we show regulates...
Abstract In this study we performed a step-wise optimization of biologically active IL-2 for delivery using E. coli Nissle 1917. Engineering the strain was coupled with an in vitro cell assay to measure biological activity microbially produced (mi-IL2). Next, assessed immune modulatory potential mi-IL2 3D tumor spheroid model demonstrating strong effect on activation. Finally, evaluated anticancer properties engineered murine CT26 model. The injected intravenously and selectively colonized...
Abstract Background CRISPR-Cas systems, which provide adaptive immunity against foreign nucleic acids in prokaryotes, can serve as useful molecular tools for multiple applications genome engineering. Diverse systems originating from distinct prokaryotes function through a common mechanism involving the assembly of small crRNA molecules and Cas proteins into ribonucleoprotein (RNP) effector complex, formation an R-loop structure upon binding to target DNA. Extensive research on I-E subtype...
Abstract Senescent cells secrete cytokines, chemokines and growth factors collectively known as the senescence-associated secretory phenotype (SASP) which can reinforce senescence activate immune response. However, it also negatively impact neighbouring tissues facilitating tumor progression. We have previously shown that in proliferating nuclear long non-coding RNA (lncRNA) MIR31HG inhibits p16/CDKN2A expression through interaction with polycomb repressor complexes (PRC1/2) during...