A5031391641- Cancer Genomics and Diagnostics
- Plant Physiology and Cultivation Studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Congenital heart defects research
- Genomics and Rare Diseases
- Plant Stress Responses and Tolerance
- Lung Cancer Treatments and Mutations
- Biomedical Text Mining and Ontologies
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Bioinformatics and Genomic Networks
- Ovarian cancer diagnosis and treatment
- Renal cell carcinoma treatment
- Plant Surface Properties and Treatments
- Biomimetic flight and propulsion mechanisms
- HER2/EGFR in Cancer Research
- Plant-Microbe Interactions and Immunity
- Berry genetics and cultivation research
- Developmental Biology and Gene Regulation
- Radiomics and Machine Learning in Medical Imaging
University of Wisconsin–Madison
2010-2023
Jackson Laboratory
2015-2021
University of Florida
2020
Children's Hospital of Eastern Ontario
2015
UConn Health
2014
Wesleyan University
2005-2012
Madison Group (United States)
2006
California Institute of Technology
1992
Auxin-binding protein 1 (ABP1) was discovered nearly 40 years ago and shown to be essential for plant development morphogenesis, but its mode of action remains unclear. Here, we report that the plasma membrane-localized transmembrane kinase (TMK) receptor-like kinases interact with ABP1 transduce auxin signal activate membrane-associated ROPs [Rho-like guanosine triphosphatases (GTPase) from plants], leading changes in cytoskeleton shape leaf pavement cells Arabidopsis. The interaction...
Abstract Abscission is an important developmental process in the life cycle of plant, regulating detachment organs from main body plant. This mechanism can be initiated response to environmental cues such as disease or pathogen, it a programmed shedding that no longer provide essential functions We have identified five novel dab (delayed floral organ abscission) mutants (dab1-1, dab2-1, dab3-1, dab3-2, and dab3-3) Arabidopsis. These each display unique anatomical physiological...
Abstract The ethylene binding domain (EBD) of the Arabidopsis thaliana ETR1 receptor is modeled as three membrane-spanning helices. We surveyed activity in different kingdoms and performed a bioinformatic analysis EBD. Ethylene confined to land plants, Chara, group cyanobacteria but largely absent other organisms, consistent with our finding that EBD-like sequences are overrepresented among plant cyanobacterial species. made amino acid substitutions 37 partially or completely conserved...
Precision medicine in oncology relies on rapid associations between patient-specific variations and targeted therapeutic efficacy. Due to the advancement of genomic analysis, a vast literature characterizing cancer-associated molecular aberrations relative relevance has been published. However, data are not uniformly reported or readily available, accessing relevant information clinically acceptable time-frame is daunting proposition, hampering connections patients appropriate options. One...
Mechanisms that govern the size of plant organs are not well understood but believed to involve both sensing and signaling at cellular level. We have isolated loss-of-function mutations in four genes comprising transmembrane kinase TMK subfamily receptor-like kinases (RLKs) Arabidopsis. These TMKs an extracellular leucine-rich-repeat motif, a single region, cytoplasmic domain. While mutants do display discernable phenotypes, unique double triple mutant combinations result severe reduction...
Understanding mechanisms underlying specific chemotherapeutic responses in subtypes of cancer may improve identification treatment strategies most likely to benefit particular patients. For example, triple-negative breast (TNBC) patients have variable response the agent cisplatin. basis will lead more informed decisions about selection strategies.In this study we used an integrative functional genomics approach investigate molecular known cisplatin-response differences among TNBC. To...
AGL15 (AGAMOUS-like 15), a member of the MADS domain family regulatory factors, accumulates preferentially throughout early stages plant life cycle. In this study, we investigated expression pattern and possible roles postembryonic accumulation AGL15. Using combination reporter genes, RNA gel blot analysis, immunochemistry, found that protein transiently in shoot apex young Arabidopsis Brassica seedlings promoter activity is associated with base leaf petioles vegetative phase. During...
Genomic and cDNA clones that code for a protein with structural biochemical properties similar to the receptor kinases from animals were obtained Arabidopsis. Structural features of predicted polypeptide include an amino-terminal membrane targeting signal sequence, region containing blocks leucine-rich repeat elements, single putative spanning domain, characteristic serine/threonine-specific kinase domain. The gene coding this receptor-like transmembrane was designated TMK1. Portions TMK1...
Abstract Background Polygalacturonases (PGs) belong to a large gene family in plants and are believed be responsible for various cell separation processes. PG activities have been shown associated with wide range of plant developmental programs such as seed germination, organ abscission, pod anther dehiscence, pollen grain maturation, fruit softening decay, xylem formation, tube growth, thus illustrating divergent roles members this family. A close look at phylogenetic relationships among...
In a screen for delayed floral organ abscission in Arabidopsis, we have identified novel mutant of CORONATINE INSENSITIVE 1 (COI1), the F-box protein that has been shown to be jasmonic acid (JA) co-receptor. While JA an important role senescence, root development, pollen dehiscence and defense responses, there little focus on its critical abscission. Abscission, or detachment organs from main body plant, is essential process during plant development unique type cell separation regulated by...
ERBB2 (HER2) is a well-studied oncogene with several driver mutations apart from the well-known amplification defect in some breast cancers. We used GigaAssay to test functional effect of HER2 missense on its receptor tyrosine kinase function. The modular high-throughput one-pot assay system for simultaneously measuring molecular function thousands genetic variants at very high accuracy. activities 5,886 were classified, significantly more than mutants previously reported. These include 112...
The process of floral organ abscission in Arabidopsis thaliana can be modulated by ethylene and involves numerous genes contributing to cell separation. One gene that is absolutely required for INFLORESCENCE DEFICIENT IN ABSCISSION, IDA, as the ida mutant completely blocked abscission. To elucidate genetic pathways regulating abscission, molecular markers expressed zone have been studied an background. Using plants with promoter-reporter constructs including promoters a novel FLORAL...
Abstract After initial patterning, muscle in the trunk and fins of teleosts grows extensively. Here, we describe growth zebrafish, with emphasis on pectoral fin musculature. In trunk, slow fibers differentiate first. contrast, does not appear until beginning juvenile period. Mosaic hyperplasia contributes to growth, new are apparent within as early 6 mm standard length. muscle, mosaic is evident at any examined stage. Instead, predominant mode stratified. larval subjacent skin, this...
Floral organ shedding is a cell separation event preceded by cell-wall loosening and generally accompanied expansion. Mutations in NEVERSHED (NEV) or INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) block floral abscission Arabidopsis thaliana. NEV encodes an ADP-ribosylation factor GTPase-activating protein, cells of nev mutant flowers display membrane-trafficking defects. IDA secreted peptide that signals through the receptor-like kinases HAESA (HAE) HAESA-LIKE2 (HSL2). Analyses single double...