A5101549832- Antibiotic Resistance in Bacteria
- MicroRNA in disease regulation
- Vagus Nerve Stimulation Research
- RNA modifications and cancer
- Cancer-related gene regulation
- Nicotinic Acetylcholine Receptors Study
- Antibiotics Pharmacokinetics and Efficacy
- Pancreatic and Hepatic Oncology Research
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Enterobacteriaceae and Cronobacter Research
- Heart Rate Variability and Autonomic Control
- Cancer-related Molecular Pathways
- Inflammatory Biomarkers in Disease Prognosis
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- Cardiac Ischemia and Reperfusion
- FOXO transcription factor regulation
- Clusterin in disease pathology
- Transcranial Magnetic Stimulation Studies
- Proteoglycans and glycosaminoglycans research
- Wnt/β-catenin signaling in development and cancer
Chongqing Medical University
2020-2024
Shanghai First People's Hospital
2002-2024
Dalian Medical University
2024
Second Affiliated Hospital of Chongqing Medical University
2024
Jiangsu Province Hospital
2009-2023
Nanjing Medical University
2006-2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2020-2023
Heilongjiang Provincial Academy of Agricultural Sciences
2023
Sichuan Agricultural University
2023
Shanghai Jiao Tong University
2009-2016
The aim of present study was to investigate the role preoperative neutrophil lymphocyte ratio (NLR) and platelet (PLR) used as prognostic markers for predicting chemotherapeutic response survival outcomes in patients with epithelial ovarian cancer (EOC) who are receivi ng platinum-based chemotherapy. A total 344 diagnosed EOC receiving chemotherapy from 2005 2010 hospital were enrolled. NLR PLR calculated complete blood cell count taken before operation. divided into platinum-resistant (P-R)...
Background: Excessive activation of matrix metalloproteinase 9 (MMP-9) has been found in several inflammatory diseases. Previous studies have shown that acetylcholine (ACh) reduced the levels pro-inflammatory cytokines and decreased tissue damage. Therefore, this study was designed to explore potential effects mechanisms ACh on MMP-9 production cell migration response lipopolysaccharide (LPS) stimulation RAW264.7 cells. Methods: expression activity were induced by LPS cells, examined...
Background: The anti-infammatory and cardioprotective effect of acetylcholine (ACh) has been reported; nevertheless, whether how ACh exhibits an antioxidant property against ischemia/reperfusion (I/R)-induced oxidative stress remains obscure. Methods: In the present study, H9c2 rat cardiomyocytes were exposed to hypoxia/reoxygenation (H/R) mimic I/R injury. We estimated intracellular different sources reactive oxygen species (ROS) by measuring mitochondrial ROS (mtROS), DNA (mtDNA) copy...
<b><i>Background:</i></b> The aim of present study was to investigate the role prognostic nutritional index (PNI) used as a marker for predicting response and survival outcomes in patients with epithelial ovarian cancer (EOC) who are receiving platinum-based chemotherapy. <b><i>Patients Methods:</i></b> Patients new diagnosis EOC postoperative chemotherapy were identified. PNI calculated 10 × serum albumin value (g/dl) + 0.005 peripheral...
Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype PK, has been proposed as new tumor marker with high expression various tissues. Here we aimed to explore effects siRNA-PKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, which PKM2 was notably expressed. gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected OVCAR3 cells...
The function and molecular biological mechanism of immediate early response 3 (IER3) on the tumorigenesis oral squamous cell carcinoma (OSCC) are aimed to be explored. effects IER3 proliferation, apoptosis, mobility OSCC cells first assessed utilizing colony formation, EdU assay, flow cytometry, transwell assay. effect glycolytic ability is validated by detecting extracellular acidification rate oxygen consumption rate. Additionally, glycolysis- wnt/β-catenin signaling-associated protein...
Recent findings have reported that up-regulation of tumor necrosis factor-alpha (TNF-α) induced by myocardial hypoxia aggravates cardiomyocyte injury. Acetylcholine (ACh), the principle vagal neurotransmitter, protects cardiomyocytes against inhibiting apoptosis. However, it is still unclear whether ACh regulates TNF-α production in after hypoxia. The concentration extracellular was increased a time-dependent manner during Furthermore, treatment also inhibited hypoxia-induced mRNA and...
Previous findings have shown that acetylcholine (ACh) decreased hypoxia-induced tumor necrosis factor alpha (TNF α) production, thus protected against cardiomyocyte injury. However, whether and how ACh affects TNF α-induced endoplasmic reticulum (ER) stress cell apoptosis remain poorly defined. This study was aimed at determining the effect of in H9c2 cells after α stimulation. Presence ER verified using protein markers glucose regulatory 78 (GRP78) C/EBP homologous (CHOP). Cell by caspase-3...
Abstract Background Ovarian cancer (OC) is a malignancy among female globally. Circular RNAs (circRNAs) are family of circular endogenous generated from selective splicing, which take part in many traits. Former investigation suggested that circ-TFRC was abnormally expressed breast (BC). Further, the role to progress OC remains unclear. So, aim this study reveal regulatory mechanism circ-TFRC. Methods Our team made luciferase reporter assay validate downstream target. Transwell migration...
The combination of hypervirulent Klebsiella pneumoniae (hvKP) infection with carbapenem and tigecycline resistance leads to significant challenges clinical treatment, limited available antibiotics poor patient prognoses. hvKP12 isolate was obtained from a blood sample 74-year-old female in Chinese teaching hospital. Whole-genome sequencing microbial characterization were performed understand the evolutionary mechanism its resistance. infected died due pyemia after 17-day treatment....
MicroRNAs (miRNAs) are small non-coding RNAs of 20-22 nucleotides (nts) and constitute a novel class gene regulators that negatively regulate expression at the post-transcriptional level. The miRNA is deregulated in many types cancers. Alterations may be an important contributor to development pancreatic carcinoma. We hypothesized genetic variations genes were associated with carcinoma analyzed genomic sequences coding for precursors eight both tissues cancer cell lines. Four mutations...
Ovarian cancer is one of the most common cancers which cause female mortality. The knowledge ovarian initiation and progression critical to develop new therapeutic strategies treat prevent it. Recently, SOX3 has been reported play a pivotal role in tumor progression. However, clinical significance human remains elusive, identity initiation, progression, related underlying mechanism unknown. In this study, we showed that expression increased from benign borderline malignant tumors....
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) has received considerable attention. Typically, the genetic elements that confer virulence are harbored by nonconjugative plasmids. In this study, we report a CR-hvKP strain, CY814036, of high-risk sequence type 25 (ST25) and K2 serotype, which is uncommon among K. isolates but caused serious lung infection in tertiary teaching hospital China. Whole-genome sequencing (WGS) revealed rare conjugative plasmid, pCY814036-iucA,...
Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous (SAC) of ovary with complementary DNA microarray. Methods: Complementary microarray was carried out in 8 CCCs SACs ovary. Differentially identified verified by real-time polymerase chain reaction. The expression protein also immunohistochemistry Western blot cells tissues ovarian CCC. Results: Comparison gene profiling 21 more than 2-fold different CCC SAC up-regulated...
This research aims to profile ten novel strains of carbapenem-resistant Enterobacteriaceae (CRE) co-carrying blaKPC and blaNDM.Clinical CRE strains, along with corresponding medical records, were gathered. To ascertain the susceptibility antibiotics, antimicrobial tests conducted. validate transferability cost fitness plasmids, conjugation experiments growth curves employed. For determining similarity between different ERIC-PCR was utilised. Meanwhile, whole genome sequencing (WGS) performed...
Purpose: Hypervirulent klebsiella pneumoniae (hvKP) is responsible for various invasive diseases and associated with high mortality. However, the clinical microbiological factors of hvKP infection that influence prognosis have not been well studied. The purpose this study was to evaluate prognostic in-hospital mortality patients infections, mainly focusing on characteristics. Methods: A retrospective conducted in strains which positive iucA string test. According outcomes during...
Adenosine, a catabolite of ATP, displays wide variety effects in the heart including regulation cardiac response to myocardial ischemia and reperfusion injury. Nonetheless, precise mechanism adenosine-induced cardioprotection is still elusive. Isolated Sprague-Dawley rat hearts underwent 30 min global 120 using Langendorff apparatus. Both adenosine acetylcholine treatment recovered post-reperfusion function associated with muscarinic receptors activation. Simultaneous administration failed...
Dysfunction of apoptosis is a significant characteristic in chronic lymphocytic leukemia (CLL). Murine double minute 4 (MDM4), miR-34a and TP53 are found to participate modulating cellular while the specific mechanism keeps unclear. This study was designed investigate potential feedback circuit among MDM4, TP53.According bioinformatic approaches, there candidate binding domains MDM4. Use dual luciferase reporter gene verify regulation between Flow cytometry used detect change level CLL cells...
copies/g RNA.The expression levels of CD44v6mRNA in peripheral blood gastric cancer patients after curative operation increased from 5.5 × 10 6 -7.6 9 RNA and the average level was 2.4×10 8 (Figure 3B) (P = 0.00496).After operation, decreased markedly. CONCLUSION:Semi-quantitative quantitative RT-PCR amplification for is a sensitive specific method detection micro-metastasis bone marrow, which might be used as an indicator tumor burden therapeutic effect.