A5049012398- Cellular Mechanics and Interactions
- Aluminum Alloys Composites Properties
- Magnesium Alloys: Properties and Applications
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Thermal properties of materials
- Tissue Engineering and Regenerative Medicine
- Silk-based biomaterials and applications
- Advanced Sensor and Energy Harvesting Materials
- Advanced Thermoelectric Materials and Devices
- Orbital Angular Momentum in Optics
- Additive Manufacturing Materials and Processes
- Graphene and Nanomaterials Applications
- Nanoplatforms for cancer theranostics
- 2D Materials and Applications
- Bone Tissue Engineering Materials
- Burkholderia infections and melioidosis
- Microfluidic and Bio-sensing Technologies
- Hippo pathway signaling and YAP/TAZ
- Advanced materials and composites
- Force Microscopy Techniques and Applications
- Additive Manufacturing and 3D Printing Technologies
- Digital Holography and Microscopy
- Electromagnetic wave absorption materials
National University of Singapore
2014-2024
Singapore Institute of Technology
2020
Singapore Institute of Manufacturing Technology
2016-2017
STMicroelectronics (Switzerland)
2008
Hyperexcitable neurons in brain organoids Individuals with Angelman syndrome experience intellectual disability and seizures throughout their lives. In this condition, ubiquitin-mediated degradation of a key potassium channel is disrupted, allowing for the neuronal excitability network synchronization that leads to seizure. Sun et al. used organoid technology study what happens human mutation ubiquitin ligase implicated syndrome. these vitro models mouse model syndrome, antagonists...
As one of the powder bed fusion additive manufacturing technologies, electron beam melting (EBM) is gaining more and attention due to its near-net-shape production capacity with low residual stress good mechanical properties. These characteristics also allow EBM built parts be used as produced without post-processing. However, as-built rough surface introduces a detrimental influence on properties metallic alloys. Thereafter, understanding effects processing parameters part's roughness, in...
Abstract Bacterial infections remain a leading threat to global health because of the misuse antibiotics and rise in drug‐resistant pathogens. Although several strategies such as photothermal therapy magneto‐thermal can suppress bacterial infections, excessive heat often damages host cells lengthens healing time. Here, localized thermal managing strategy, thermal‐disrupting interface induced mitigation (TRIM), is reported, minimize intercellular cohesion loss for accurate antibacterial...
Abstract Porous materials provide a large surface‐to‐volume ratio, thereby providing knob to alter fundamental properties in unprecedented ways. In thermal transport, porous nanomaterials can reduce conductivity by not only enhancing phonon scattering from the boundaries of pores and therefore decreasing mean free path, but also reducing group velocity. Herein, structure–property relationship is established measuring porosity individual electrolessly etched single‐crystalline silicon...
Ti-6Al-4V (Ti64) parts of varying thicknesses were additively manufactured (AM) by the powder-bed-based electron beam melting (EBM) technique. Microstructure and wear properties these EBM-built have been investigated in comparison with conventionally cast Ti64 samples. Sliding tests conducted using a ball-on-disc micro-tribometer under ambient conditions. Experimental results reveal that samples exhibited higher microhardness an overall larger coefficient friction as compared to as-cast...
Black phosphorus (BP) has emerged as a promising candidate for next-generation electronics and optoelectronics among the 2D family materials due to its extraordinary electrical/optical/optoelectronic properties. Interestingly, BP shows strong anisotropic transport behavior because of puckered honeycomb structure. Previous studies have demonstrated thermal anisotropy theoretically attribute this in both phonon dispersion relation relaxation time. However, exact origin such lacks clarity yet...
Abstract An orchestrated wound healing program drives skin repair via collective epidermal cell proliferation and migration. However, the molecular determinants of tissue microenvironment supporting remain poorly understood. Herein we discover that proteoglycan Agrin is enriched within early wound-microenvironment indispensable for efficient healing. enhances mechanoperception keratinocytes by augmenting their stiffness, traction stress fluidic velocity fields in retaliation to bulk...
In this work, nanovoid-enhanced thin-film composite (TFC) membranes have been successfully fabricated using ZIF-67 nanoparticles as the sacrificial template. By incorporating different amounts of during interfacial polymerization, resultant TFC can degrees nanovoids after self-degradation in water, consequently influencing their physiochemical properties and separation performance. Nanovoid structures endow with additional passages for water molecules. Thus, all newly developed exhibit...
Abstract This review examines the significant role of Atomic Force Microscopy (AFM) in neurobiological research and its emerging clinical applications diagnosing neurological disorders central nervous system (CNS) tumours. AFM, known for nanometre-scale resolution piconewton-scale force sensitivity, offers ground breaking insights into biomechanical properties brain cells tissues their interactions within microenvironment. delves application AFM non-clinical settings, where it characterizes...
Collective cell migration is crucial in various physiological processes, including wound healing, morphogenesis, and cancer metastasis. Adherens Junctions (AJs) play a pivotal role regulating cohesion dynamics during tissue remodeling. While the origin of junctional mechanical tension at AJs have been extensively studied, influence actin cortex structure on junction plasticity remains incompletely understood. Moreover, mechanisms underlying stress dissipation junctions are not well...
Chromatin architecture is critical in determining nuclear mechanics. Most studies focus on the mechanical rigidity conferred by chromatin compaction from densely packed heterochromatin, but less known how transient changes decompaction state impinge nucleus stiffness. Here, we used an array of vertically aligned nanopillars to study deformability situ after cells. The significantly stiffened within 4 hours softened at longer timescales. This acute stiffening was predominantly underlied...
Tracking fast-swimming bacteria in three dimensions can be extremely challenging with current optical techniques and a microscopic approach that rapidly acquire volumetric information is required. Here, we introduce phase-contrast holographic video microscopy as solution for the simultaneous tracking of multiple fast moving cells dimensions. This technique uses interference patterns formed between scattered incident field to infer three-dimensional (3D) position size bacteria. Using this...
The role of biophysical induction methods such as cell micropatterning in stem differentiation has been well documented previously. However, the underlying mechanistic linkage engineered shape to directed lineage commitment remains poorly understood. Here, it is reported that plays an important regulating optimal cytoskeletal tension development human mesenchymal (hMSC) via mechanotransduction pathways induce cardiomyogenic differentiation. Cells are grown on fibronectin strip patterns...
Re-epithelialization by collective migration of epithelial cells over a heterogeneous environment to restore tissue integrity and functions is critical for development regeneration. Here, it reported that the spatial organization adjacent adherent paths within sparsely distributed extracellular matrix (ECM) has significant impact on orientational coupling between cell polarization migration. This effect determines pattern human keratinocytes regain their cohesion, which impacts occupancy...
A key hallmark of many diseases, especially those in the central nervous system (CNS), is change tissue stiffness due to inflammation and scarring. However, how such changes microenvironment affect regenerative process remains poorly understood. Here, a biomimicking fiber platform that provides independent variation structural intrinsic reported. To demonstrate functionality these constructs as mechanotransduction study platform, substrates are utilized artificial axons effects axon versus...
In the present study, Mg (1.98 and 2.5) vol % TiO₂ nanocomposites are primarily synthesized utilizing solid-phase blend-press-sinter powder metallurgy (PM) technique liquid-phase disintegrated melt deposition (DMD) followed by hot extrusion. Microstructural characterization of Mg-TiO₂ indicated significant grain refinement with DMD exhibiting as high ~47% for 2.5 NPs addition. X-ray diffraction studies that texture randomization pure depends not only on critical amount added to matrix but...
Purpose: To evaluate the biomechanical properties (Young's modulus) of normal (control) and glaucomatous human iris using atomic force microscopy (AFM). Methods: Iris tissue obtained from eighteen subjects (equal number eyes with primary angle closure glaucoma (PACG) open (POAG) five who underwent elective eye surgery were subjected to estimation Young's modulus by AFM. Force measurements done at room temperature Nanowizard II BioAFM. The samples immersed in liquid media (PBS 0.1% BSA)...
There has been an increased use of silicone gel for applications such as cell traction force measurements well lab- and organ-on-chip systems. However, is a viscoelastic material which tends to undergo non-elastic deformation displays time-dependent strain rate-dependent responses. Here, we evaluated the mechanical responses two types commonly used gels, Sylgard-184 CY52-276, when subjected nanoNewton micrometer displacement length scales. Using different characterization tools theoretical...
A hydrogel drop-screen device was developed to rapidly measure large-scale single-adherent cell morphologies and multiple protease secretions on gelatin particles with a throughput ∼100 cells per second for phenotype profiling.
ABSTRACT Proprietary mixture of polyurethane methacrylate (PUMA) and off‐stoichiometry thiol‐ene (OSTE‐80) are evaluated as two possible polymeric substrates to prototype microfluidic biochips. Because their lack biocompatibility, PUMA OSTE‐80 modified by argon (Ar) or nitrogen (N 2 ) plasma treatment introduce moieties that highly polar conducive for cell attachment growth. XPS water contact angle measurement show these groups relatively stable in the plasma‐treated spite hydrophobic...