A5047584503- CRISPR and Genetic Engineering
- Wound Healing and Treatments
- Silk-based biomaterials and applications
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- Gene expression and cancer classification
- Global Public Health Policies and Epidemiology
- RNA Research and Splicing
- Tendon Structure and Treatment
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Plant Molecular Biology Research
- Genetic Syndromes and Imprinting
- BRCA gene mutations in cancer
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- Genetic Associations and Epidemiology
- Cardiovascular Health and Risk Factors
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Cellular Mechanics and Interactions
- RNA modifications and cancer
Genome Institute of Singapore
2022-2024
Agency for Science, Technology and Research
2018-2022
Institute of Molecular and Cell Biology
2018-2022
National University of Singapore
2018-2019
The extent of and the oncogenic role played by alternative splicing (AS) in cancer are well documented. Nonetheless, only few studies have attempted to dissect individual gene function at an isoform level. Here, we focus on AS factors during prostate progression, as these known undergo extensive potential affect hundreds downstream genes. We identified exon 7 (ex7) MBNL1 (Muscleblind-like 1) transcript being most differentially included cancer, both cell lines patients' samples. In contrast,...
Abstract An orchestrated wound healing program drives skin repair via collective epidermal cell proliferation and migration. However, the molecular determinants of tissue microenvironment supporting remain poorly understood. Herein we discover that proteoglycan Agrin is enriched within early wound-microenvironment indispensable for efficient healing. enhances mechanoperception keratinocytes by augmenting their stiffness, traction stress fluidic velocity fields in retaliation to bulk...
Abstract Asian people are under-represented in population-based, clinical, and genomic research. 1,2 To address this gap, we have initiated the HELIOS longitudinal cohort study, comprising comprehensive behavioural, phenotypic, measurements from 10,004 men women of Chinese, Indian or Malay background. Phenotyping has been carried out using validated approaches, that internationally interoperable. Health record linkage enriches both baseline phenotyping evaluation prospective outcomes. The...
The transcriptional co-regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are the vertebrate downstream effectors of Hippo signaling pathway that controls various physiological pathological processes. pair TEAD (TEA domain) family transcription factors to initiate transcription. We previously identified a tractable pocket in TEADs, which has been physiologically shown bind palmitate. Herein, TEAD-palmitate interaction screen was developed...
Global epigenetic reprogramming is vital to purge germ cell-specific features establish the totipotent state of embryo. This process transpires be carefully regulated and not an undirected, radical erasure parental epigenomes. The TRIM28 complex has been shown crucial in embryonic by regionally opposing DNA demethylation preserve information inherited from germline soma. Yet DNA-binding factors guiding this specific targets are largely unknown. Here, we uncover characterize a novel,...
Purpose We evaluated differences in a 313-variant breast cancer polygenic risk score (PRS313) across genomic platforms and their impact on stratification. Methods compared PRS313 derived from genotyping arrays (Global Screening Array [GSA], OncoArray-500K [OncoArray], Global Diversity [GDA], custom Axiom_PrecipV1 array [ThermoFisher]) low-coverage genome sequencing (lc-WGS) 2 cell lines 92 individuals. Probes were designed for all variants ThermoFisher (success rate: 259/313). Sanger was...
Mitchell-Riley syndrome (MRS) is caused by recessive mutations in the regulatory factor X6 gene (RFX6) and characterised pancreatic hypoplasia neonatal diabetes. To determine why individuals with MRS specifically lack endocrine cells, we micro-CT imaged a 12-week-old foetus homozygous for nonsense mutation RFX6 c.1129C>T, which revealed loss of pancreas body tail. From this foetus, derived iPSCs show that differentiation these cells vitro proceeds normally until generation endoderm,...
Histone acetylation and methylation are epigenetic modifications that dynamically regulated by chromatin modifiers to precisely regulate gene expression. However, the interplay which histone synchronized coordinate cellular differentiation is not fully understood. In this study, we demonstrate a relationship between BRD4, reader of marks, G9a, writer marks in regulation myogenic differentiation. Using loss- gain-of-function studies, as well pharmacological inhibition its activity, examined...