A5080955609- Pancreatic function and diabetes
- Diabetes and associated disorders
- Renal and related cancers
- Diabetes Management and Research
- SARS-CoV-2 detection and testing
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Diabetes Treatment and Management
- Genomics and Chromatin Dynamics
- Additive Manufacturing Materials and Processes
- Biosensors and Analytical Detection
- Urological Disorders and Treatments
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Racial and Ethnic Identity Research
- Blood transfusion and management
- Global Energy and Sustainability Research
- Dementia and Cognitive Impairment Research
- Restraint-Related Deaths
- High Entropy Alloys Studies
- Genetic Syndromes and Imprinting
- Healthcare and Environmental Waste Management
- Gynecological conditions and treatments
- COVID-19 diagnosis using AI
- Aortic Disease and Treatment Approaches
University of Manchester
2014-2024
Manchester Academic Health Science Centre
2013-2024
Manchester University NHS Foundation Trust
2015-2024
Cooper University Hospital
2023
UnitedHealth Group (United States)
2020
Applied Research Associates (United States)
2020
Temple University
2017-2019
Southampton General Hospital
2019
Essex Cardiothoracic Centre
2019
Manchester University
2014-2016
The observed diversity of dinosaurs reached its highest peak during the mid- and Late Cretaceous, 50 Myr that preceded their extinction, yet this explosion dinosaur may be explained largely by sampling bias. It has long been debated whether were part Cretaceous Terrestrial Revolution (KTR), from 125–80 ago, when flowering plants, herbivorous social insects, squamates, birds mammals all underwent a rapid expansion. Although an apparent occurred in mid-Cretaceous, coinciding with emergence new...
Knowledge of human pancreas development underpins our interpretation and exploitation pluripotent stem cell (PSC) differentiation toward a β-cell fate. However, almost no information exists on the early events pancreatic specification in distal foregut, bud formation, development. Here, we have studied expression profiles key lineage-specific markers to understand morphogenetic during The notochord was adjacent dorsal foregut endoderm fourth week before duodenal homeobox-1 detection. In...
Urinary bladder malformations associated with outlet obstruction are a frequent cause of progressive renal failure in children. We here describe muscarinic acetylcholine receptor M3 (CHRM3) (1q41-q44) homozygous frameshift mutation familial congenital malformation prune-belly-like syndrome, defining an isolated gene defect underlying this sometimes devastating disease. CHRM3 encodes the receptor, which we show is present developing epithelia and muscle. These observations may imply that has...
Human organogenesis is when severe developmental abnormalities commonly originate. However, understanding this critical embryonic phase has relied upon inference from patient phenotypes and assumptions in vitro stem cell models non-human vertebrates. We report an integrated transcriptomic atlas of human organogenesis. By lineage-guided principal components analysis, we uncover novel relatedness particular genes across different organs tissues identified unique transcriptional codes which...
Identifying genes linked to extreme phenotypes in humans has the potential highlight biological processes not shared with all other mammals. Here, we report identification of homozygous loss-of-function variants primate-specific gene ZNF808 as a cause pancreatic agenesis. is member KRAB zinc finger protein family, large and rapidly evolving group epigenetic silencers which target transposable elements. We show that loss vitro results aberrant activation regulatory contained elements it...
The basic helix-loop-helix transcription factor, NEUROG3, is critical in causing endocrine commitment from a progenitor cell population the developing pancreas. In human, NEUROG3 has been detected 8 weeks post-conception (wpc). However, profile of its production and when it ceases to be unknown. this study we have defined detection pancreas give insight into NEUROG3-dependent possible human fetus. Immunohistochemistry allowed counting cells with positively stained nuclei 7 wpc through term....
To interrogate the alternative fates of pancreas and liver in earliest stages human organogenesis, we developed laser capture, RNA amplification, computational analysis deep sequencing. Pancreas-enriched gene expression was less conserved between mouse than for liver. The dorsal pancreatic bud enriched components Notch, Wnt, BMP, FGF signaling, almost all genes known to cause agenesis or hypoplasia, over 30 unexplored transcription factors. SOX9 RORA were imputed as key regulators compared...
Powder smoke phenomena – a cloud of metallic powder created by electrostatic repulsion is an inherent issue that has been observed in many high temperature alloys processed Electron Beam Bed Fusion (EPBF). To date the majority research addressing this focused on machine modification and process manipulation to prevent overcharging particles, but little investigated effects controlling characteristics. A recent body work from University Tohoku proposed ball milling as method for mechanically...
How the genome activates or silences transcriptional programmes governs organ formation. Little is known in human embryos undermining our ability to benchmark fidelity of stem cell differentiation programming, interpret pathogenicity noncoding variation. Here, we study histone modifications across thirteen tissues during organogenesis. We integrate data with transcription build an overview how differentially regulates alternative fates including by repression. Promoters from nearly 20,000...
Refugee and immigrant populations are extremely vulnerable to the consequences of COVID-19 pandemic. vaccination is a critical tool in mitigating these consequences, but same communities often lack access vaccines. We describe efforts community-based primary care clinic Clarkston, Georgia provide vaccines culturally sensitive manner address this health disparity vaccine hesitancy.
In order to gain insight into the pathogenesis of frontotemporal lobar degeneration (FTLD), mean tau load in frontal cortex was compared 34 patients with dementia linked chromosome 17 (FTDP‐17) 12 different mutations gene ( MAPT ), 11 sporadic FTLD Pick bodies and 25 early onset Alzheimer’s disease (EOAD). Tau determined, as percentage tissue occupied by stained product, image analysis immunohistochemically sections using phospho‐dependent antibodies AT8, AT100 AT180. With AT8 AT180...
Diffuse congenital hyperinsulinism in infancy (CHI-D) arises from mutations inactivating the KATP channel; however, phenotype is difficult to explain electrophysiology alone. Here we studied wider abnormalities β-cell and other pancreatic lineages. Islets were disorganized CHI-D compared with controls. PAX4 ARX expression was decreased. A tendency toward increased NKX2.2 consistent its detection two-thirds of δ-cell nuclei, similar fetal pancreas, implied immature function. δ-cells also...
Mitchell-Riley syndrome (MRS) is caused by recessive mutations in the regulatory factor X6 gene (RFX6) and characterised pancreatic hypoplasia neonatal diabetes. To determine why individuals with MRS specifically lack endocrine cells, we micro-CT imaged a 12-week-old foetus homozygous for nonsense mutation RFX6 c.1129C>T, which revealed loss of pancreas body tail. From this foetus, derived iPSCs show that differentiation these cells vitro proceeds normally until generation endoderm,...
Background: Endometriosis is a multifactorial benign disorder characterized by the abnormal presence of endometrial tissue in an extraendometrial site. Although extrapelvic endometriosis uncommon, symptomatic involvement kidney exceedingly rare. This disease can mimic several urologic processes, but because its scarcity clinical practice, it seldom considered differential. Case Presentation: In this report, we describe case 45-year-old woman with flank pain and hematuria, who was found to...
Abstract Cell proliferation is fundamental for almost all stages of development and differentiation that require an increase in cell number. Although cycle phase has been associated with differentiation, the actual process not considered as having a specific role. Here we exploit human embryonic stem cell-derived endodermal progenitors find are vitro model ventral foregut. These cells exhibit expansion-dependent increases efficiency to pancreatic linked organ-specific enhancer priming at...
Both polyester and foam nasal swabs were collected from convalescent COVID-19 patients at a single visit stored in viral transport media (VTM), saline or dry. Sensitivity of each swab material combination estimated, three by tables constructed to measure concordance, cycle threshold (Ct) values compared. 126 visits had 51 saline, 63 VTM dry tube. Polyester an estimated sensitivity 87.3% 94.5% respectively VTM, 87.5% 93.8% 75.0% 90.6% for VTM. Ct correlated, but showed decreased performance...