Xylanases have drawn much attention owing to possessing great potential in various industrial applications. However, the applicability of xylanases, exemplified by production bioethanol and xylooligosaccharides (XOSs), was bottlenecked their low stabilities at higher temperatures. The main purpose this work improve thermostability AuXyn11A, a mesophilic glycoside hydrolase (GH) family 11 xylanase from Aspergillus usamii E001, N-terminus replacement.A hybrid with high thermostability, named...

The contribution of disulfide bridges to the thermostability a type A feruloyl esterase (AuFaeA) from Aspergillus usamii E001 was studied by introducing an extra bridge or eliminating native one enzyme. MODIP and DbD, two computational tools that can predict possible in proteins for improvement, molecular dynamics (MD) simulations were used design bridge. One residue pair A126-N152 chosen, respective amino acid residues mutated cysteine. wild-type AuFaeA its variants expressed Pichia...

The AuMan5A, an acidophilic glycoside hydrolase (GH) family 5 β-mannanase derived from Aspergillus usamii YL-01-78, consists of only catalytic domain (CD). To perfect enzymatic properties the a 1 carbohydrate-binding module (CBM) Trichoderma reesei cellobiohydrolase I (TrCBH I), having lowest binding free energy with cellobiose, was selected by in silico design, and fused into its C-terminus forming fusion β-mannanase, designated as AuMan5A-CBM. Then, encoding gene, Auman5A-cbm, constructed...

Background: Exosomes are essential for hepatocellular carcinoma (HCC) progression and have garnered significant interest as novel targets diagnostic, prognostic, therapeutic approaches. This study aims to identify potential exosome-related biomarkers the development of useful strategies HCC diagnosis therapy. Methods: Three datasets obtained from Gene Expression Omnibus (GEO) were utilized differentially expressed genes (DEGs) in HCC. Through Ontology (GO) analysis protein–protein...

A cDNA fragment of the Anman5A, a gene that encodes an acidophilic β-mannanase Aspergillus niger LW-1 (abbreviated as AnMan5A), was cloned and functionally expressed in Pichia pastoris . Homology alignment amino acid sequences verified AnMan5A belongs to glycoside hydrolase (GH) family 5. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay demonstrated recombinant (reAnMan5A), N-glycosylated protein with apparent molecular weight 52.0 kDa, secreted into medium. The...

Xylanases have attracted much attention owing to their potential applications. The applicability of xylanases, however, was bottlenecked by low stabilities at high temperature or extreme pH. purpose this work enhance the thermostability a mesophilic xylanase N-terminal replacement.The AoXyn11, family 11 from Aspergillus oryzae, enhanced replacing its segment with corresponding one EvXyn11(TS) , hyperthermotolerant xylanase. A hybrid thermostability, NhXyn11⁵⁷, predicted molecular dynamics...

Both the activity and regioselectivity of <italic>Phaseolus vulgaris</italic> epoxide hydrolase were remarkably improved <italic>via</italic> reshaping two substrate tunnels based on rational design.

Abstract A β‐1,3‐1,4‐glucanase gene ( Auglu 12A) from Aspergillus usamii was successfully expressed in Escherichia coli BL21(DE3). The recombinant enzyme, re Au glu12A efficiently purified using the one‐step nickel‐nitrilotriacetic acid affinity chromatography. specific activity of 694.8 U/mg, with an optimal temperature 55°C and pH 5.0. exhibited stability at temperatures up to 60°C within range 4.0–5.5. hydrolytic increased presence metal ions, especially K + Na , whereas it a m V max 8.35...

To provide more options for the stereoselective hydrolysis of epoxides, an epoxide hydrolase (VrEH3) gene from Vigna radiata was cloned and expressed in Escherichia coli. Recombinant VrEH3 displayed maximum activity at pH 7.0 45 °C high stability 4.5-7.5 55 °C. Notably, reVrEH3 exhibited complementary regioselectivity toward styrene oxides 1a-3a enantioselectivity (E = 48.7) o-cresyl glycidyl ether 9a. elucidate these interesting phenomena, interactions three-dimensional structure between...

Structure-guided microtuning of an Aspergillus usamii epoxide hydrolase was executed. One mutant, A214C/A250I, displayed a 12.6-fold enhanced enantiomeric ratio (E = 202) toward rac-styrene oxide, achieving its nearly perfect kinetic resolution at 0.8 M in pure water or 1.6 n-hexanol/water. Several other beneficial mutants also significantly improved E values, offering promising biocatalysts to access 19 structurally diverse chiral monosubstituted epoxides (97.1 – ≥ 99% ees) and vicinal...

ABSTRACT: Utilization of phenolic acids, including gallic acid, coumaric caffic cinnamic and ferulic for methanol reduction in wine was investigated. Enzyme activities pectinesterase pectin lyase decreased significantly when 0.1 mg/L or acid added. However, no inhibition on polygalacturonase activity observed 0.5 Methanol content commercial pectic enzyme (CPE) group increased from 11.53 ± 1.34 to 56.67 3.75 ppm the final products. Adding with CPE inhibited increase production. In addition,...