A5088977376- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Genomics and Chromatin Dynamics
- GDF15 and Related Biomarkers
- RNA modifications and cancer
- Protein Hydrolysis and Bioactive Peptides
- Ubiquitin and proteasome pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Nutrition and Health in Aging
- CRISPR and Genetic Engineering
- Synthesis and bioactivity of alkaloids
- Mass Spectrometry Techniques and Applications
- Cardiac Fibrosis and Remodeling
- Advanced Proteomics Techniques and Applications
Zhejiang Academy of Medical Sciences
2025
Chinese Academy of Sciences
2021-2025
Jiangnan University
2015-2025
Southwest Medical University
2025
University of Chinese Academy of Sciences
2022-2024
Zhejiang Cancer Hospital
2022-2024
Beijing Institute of Technology
2024
Shanghai Jiao Tong University
2021-2023
Tianjin University
2023
Cancer Hospital of Chinese Academy of Medical Sciences
2022
Abstract Triple negative breast cancer (TNBC) is a deadly form of due to the development resistance chemotherapy affecting over 30% patients. New therapeutics and companion biomarkers are urgently needed. Recognizing elevated expression glucose transporter 1 (GLUT1, encoded by SLC2A1 ) associated metabolic dependencies in TNBC, we investigated vulnerability TNBC cell lines patient-derived samples GLUT1 inhibition. We report that genetic or pharmacological inhibition with BAY-876 impairs...
Abstract Triple-negative breast cancer (TNBC) is the most aggressive subtype with worst prognosis and few effective therapies. Here we identified MS023, an inhibitor of type I protein arginine methyltransferases (PRMTs), which has antitumor growth activity in TNBC. Pathway analysis TNBC cell lines indicates that activation interferon responses before after MS023 treatment a functional biomarker determinant response, these observations extend to panel human-derived organoids. Inhibition PRMT...
Bromodomains (BRDs) are conserved protein interaction modules which recognize (read) acetyl-lysine modifications, however their role(s) in regulating cellular states and potential as targets for the development of targeted treatment strategies is poorly understood. Here we present a set 25 chemical probes, selective small molecule inhibitors, covering 29 human bromodomain targets. We comprehensively evaluate selectivity this probe-set using BROMOscan demonstrate utility identifying roles...
Abstract Selective protein degradation platforms have opened novel avenues in therapeutic development and biological inquiry. Antibody‐based lysosome‐targeting chimeras (LYTACs) emerged as a promising technology that extends the scope of targeted to extracellular targets. Aptamers offer an advantageous alternative owing their potential for modification manipulation toward multivalent state. In this study, chemically engineered platform aptamer‐based LYTACs (AptLYTACs) is established either...
ABSTRACT Loss of RB1 function represents a defining characteristic triple‐negative breast cancer (TNBC) and is intricately associated with resistance to therapeutic interventions. In this study, we investigate the epigenetic mechanisms governing expression in TNBC. Employing combination bioinformatics analyses experimental validations, identified lysine histone methyltransferase EZH2 as key upstream regulator expression. primarily mediates trimethylation 27 on H3 catalytic subunit Polycomb...
Brain injury is a familiar complication of severe sepsis, in which excessive inflammation and oxidative stress are the main mechanisms leading to acute brain injury. Here, we focus on probing function mechanism Matairesinol (Mat) sepsis-mediated We established rat sepsis model by cecal ligation perforation (CLP) constructed an vitro treating neurons microglia with lipopolysaccharide (LPS). Rats cells were treated varying concentrations Mat, changes neural function, neuronal apoptosis,...
PRDM9 is a PR domain containing protein which trimethylates histone 3 on lysine 4 and 36. Its normal expression restricted to germ cells attenuation of its activity results in altered meiotic gene transcription, impairment double-stranded breaks pairing between homologous chromosomes. There growing evidence for role aberrant oncogenesis genome instability. Here we report the discovery MRK-740, potent (IC50: 80 ± 16 nM), selective cell-active inhibitor (Chemical Probe). MRK-740 binds...
Abstract A low‐cost, high‐precision, and secure real‐time system for monitoring food freshness can significantly improve spoilage issues, yet traditional colorimetric sensor arrays often suffer from chemical dyes’ high toxicity limited color changes. Here, a metal‐polyphenol network array (MPN‐CSA) is built detecting total volatile base nitrogen (TVB‐N) markers of meat freshness. The multi‐level competitive coordination process between the amine substances endows with changes far beyond...
We present an ambient mass spectrometry immunoassay platform based on specific tags detected by laser ablation-dielectric barrier discharge ionization-mass (LA-DBDI-MS). It features high sensitivity, multiplexed quantitation, minimal sample consumption, and convenient operation. The organic small-molecule allow very sensitivity quantitative detection of multiple proteins, even membrane-bound proteins cell surfaces, through signal amplification (approximately 600 times). By using just 2 μL...
Unchecked epithelial cell death is fundamental to the pathogenesis of pneumonia. The recognition unique signaling pathways that preserve viability may present new opportunities for interventional strategies. We describe mortality factor 4 like 1 (Morf4l1), a protein involved in chromatin remodeling, constitutively expressed at low levels lung because its continuous degradation mediated by an orphan ubiquitin E3 ligase subunit, Fbxl18. Expression Morf4l1 increases humans with pneumonia and...
Abstract Background Immune‐checkpoint blockade (ICB) therapy shows promise for treating aggressive triple‐negative breast cancer (TNBC). However, only some patients benefit from ICB, revealing an urgent need identifying novel strategies sensitizing to ICB. Previously, the authors demonstrated that type‐I protein arginine methyltransferases (PRMTs) regulated antiviral innate‐immune responses in TNBC by altering RNA splicing. This study aimed explore effects of targeting PRMTs on tumor...
Despite the well-established significance of transcription factors (TFs) in pathogenesis, their utilization as pharmacological targets has been limited by inherent challenges modulating protein interactions. The lack defined small-molecule binding pockets and nuclear localization TFs do not favor use traditional tools. Aptamers possess large molecular weights, expansive blocking surfaces efficient cellular internalization, making them compelling tools for TF Here, we report a...
The identification of drug metabolites is very important in development. Nowadays, the most widely used methods are isotopes and mass spectrometry. However, commercial isotopic labeled reagents usually expensive, rapid convenient still difficult. In this paper, an (18)O isotope labeling strategy was developed were as a tool to identify using Curcumin selected model evaluate established method, curcumin successfully synthesized. non-labeled simultaneously metabolized human liver microsomes...
The global health emergency generated by coronavirus disease-2019 has prompted the search for immunomodulatory agents.