A5091723789- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Pharmacogenetics and Drug Metabolism
- Amino Acid Enzymes and Metabolism
- Analytical Chemistry and Chromatography
- Eicosanoids and Hypertension Pharmacology
- Identification and Quantification in Food
- Biochemical Acid Research Studies
- Meat and Animal Product Quality
- Advanced Chemical Sensor Technologies
- Carbohydrate Chemistry and Synthesis
- Educational Technology and Pedagogy
- Pancreatic function and diabetes
- E-commerce and Technology Innovations
- Innovative Microfluidic and Catalytic Techniques Innovation
- Helminth infection and control
- Cyclopropane Reaction Mechanisms
- Network Security and Intrusion Detection
- Metabolism and Genetic Disorders
- Asymmetric Hydrogenation and Catalysis
- Radiomics and Machine Learning in Medical Imaging
- Photosynthetic Processes and Mechanisms
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Medical Image Segmentation Techniques
Xuchang University
2022-2024
Johns Hopkins University
2024
Jiangnan University
2017-2022
Guizhou University
2022
Wuxi Institute of Technology
2020
Both the activity and regioselectivity of <italic>Phaseolus vulgaris</italic> epoxide hydrolase were remarkably improved <italic>via</italic> reshaping two substrate tunnels based on rational design.
Structure-guided microtuning of an Aspergillus usamii epoxide hydrolase was executed. One mutant, A214C/A250I, displayed a 12.6-fold enhanced enantiomeric ratio (E = 202) toward rac-styrene oxide, achieving its nearly perfect kinetic resolution at 0.8 M in pure water or 1.6 n-hexanol/water. Several other beneficial mutants also significantly improved E values, offering promising biocatalysts to access 19 structurally diverse chiral monosubstituted epoxides (97.1 – ≥ 99% ees) and vicinal...
Single copy thaumatin-like protein (TLP) genes and multiple internal transcribed spacer (ITS) from the nucleus of corn were selected as targets, designed screened primers. The impact target sequence selection on ladder-shape melting temperature isothermal amplification (LMTIA) detection components in edible oil aspects such specificity, sensitivity, stability studied. results showed that LMTIA system targeting TLP has an optimal reaction 58 °C can detect a minimum 100 pg/μL DNA, established...
Abstract Background Epoxide hydrolase can regioselectively catalyze the oxirane ring-opening hydrolysis of rac -epoxides producing corresponding chiral diols. In our laboratory, a gene named pveh1 encoding an EH from Phaseolus vulgaris was cloned. Although directed modification Pv EH1 carried out, mutant Y3 showed limited degree enantioconvergence towards racemic ( -) m -chlorostyrene oxide CSO). Results and were combinatively subjected to laboratory evolution further enhance - CSO. Firstly,...
This work engineered a superior double-site mutant <italic>Sl</italic>EH1<sup>W106T/F189L</sup> used for the enantioconvergent biosynthesis of (<italic>R</italic>)-<bold>1b–6b</bold> with high ee<sub>p</sub> values.
A putative Solanum lycopersicum epoxide hydrolase, SlEH2, was discovered based on computer-aided analysis. Then, its encoding gene (sleh2) expressed in E. coli BL21(DE3). The substrate spectrum assay exhibited that coli/sleh2, an transformant expressing possessed the activity of 74.8 U/g wet cell and enantiomeric ratio (E) 145 towards racemic (rac-) para-chlorostyrene oxide (4a). scale-up kinetic resolution 400 mM rac-4a conducted using 200 mg/mL cells coli/sleh2 presence 1% (v/v) Tween-20,...
In the process of development modern society, network text analysis system is more and important, research in field natural language processing has been comprehensively developed. this process, mainly uses deep learning technology to realize technology, through different neural networks complete model construction, based on analysis, using word segmentation sentence classification methods. The long sentences are processed ensure that computer can better understand semantics system. Based...
To improve the productivity of L-phenyllactic acid (L-PLA), L-LcLDH1(Q88A/I229A), a Lactobacillus casei L-lactate dehydrogenase mutant, was successfully expressed in Pichia pastoris GS115. An NADH regeneration system vitro then constructed by coupling recombinant (re) LcLDH1(Q88A/I229A) with glucose 1-dehydrogenase for asymmetric reduction phenylpyruvate (PPA) to L-PLA. SDS-PAGE analysis showed that apparent molecular weight reLcLDH1(Q88A/I229A) 36.8 kDa. And its specific activity 270.5...