A5048085772- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Renal function and acid-base balance
- Electrolyte and hormonal disorders
- Amino Acid Enzymes and Metabolism
- Bone and Dental Protein Studies
- Nitric Oxide and Endothelin Effects
- Pancreatic function and diabetes
- Potassium and Related Disorders
- Enzyme Structure and Function
- Diet and metabolism studies
- Drug Transport and Resistance Mechanisms
- Lipid Membrane Structure and Behavior
- Biomedical and Chemical Research
- Erythrocyte Function and Pathophysiology
- dental development and anomalies
- Neuroscience and Neuropharmacology Research
- Membrane-based Ion Separation Techniques
- Metabolism and Genetic Disorders
- Peptidase Inhibition and Analysis
- Corneal Surgery and Treatments
- Biomedical Research and Pathophysiology
- Photoreceptor and optogenetics research
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
University of California, Los Angeles
2016-2025
Smith-Kettlewell Eye Research Institute
2023
University of Rochester Medical Center
2023
UCLA Health
2009-2022
UCLA Medical Center
1989-2020
RELX Group (United States)
2019
Ronald Reagan UCLA Medical Center
2013
Bial (Portugal)
2010
Laboratoire Informatique d'Avignon
2010
Stahl-Zentrum (Germany)
2009
BackgroundIn recent years, significant breakthroughs have been made in the field of natural language processing, particularly with development large models (LLMs). LLMs demonstrated remarkable capabilities on benchmarks related to general medical question answering, but there are fewer data about their performance subspecialty fields and studies still comparing many available LLMs. These potential be used as a part adaptive physician training, copilot applications, digital patient...
We report the cloning, sequence analysis, tissue distribution, functional expression, and chromosomal localization of human pancreatic sodium bicarbonate cotransport protein (pancreatic NBC (pNBC)). The transporter was identified by searching expressed tag data base. An I.M.A.G.E. clone W39298 identified, a polymerase chain reaction probe generated to screen pancreas cDNA library. pNBC encodes 1079-residue polypeptide that differs at N terminus from recently cloned cotransporter isolated...
Previous functional studies have demonstrated that muscle intracellular pH regulation is mediated by sodium-coupled bicarbonate transport, Na+/H+ exchange, and Cl−/bicarbonate exchange. We report the cloning, sequence analysis, tissue distribution, genomic organization, analysis of a new member sodium cotransporter (NBC) family, NBC3, from human skeletal muscle. mNBC3 encodes 1214-residue polypeptide with 12 putative membrane-spanning domains. The ∼ 7.8-kilobase transcript expressed uniquely...
The mitogenic effect of epidermal growth factor (EGF) and the characteristics EGF binding were studied on primary cultures rabbit proximal tubular cells. was found to be a potent mitogen stimulated DNA synthesis 18-fold above level observed in quiescent Using 125I-EGF as ligand, two classes specific receptors identified cell culture, i.e., high-affinity receptor with dissociation constant (Kd) 0.3 nM maximal (Bmax) 1.7 X 10(4) receptors/cell low-affinity Kd 1.9 Bmax 5.3 receptors/cell....
The microbiome has proven to influence health and disease, but how combinations of external factors affect the is relatively unknown. Diet can cause changes, this usually achieved by altering macronutrient ratios not focused on dietary protein source or saturated fat intake levels. In addition, each individual’s unique profile be an important factor during studies, it even been shown therapeutic outcomes. We show here that effects individual differences outweighed effect experimental diets...
Abstract Na + -coupled acid–base transporters play essential roles in human biology. Their dysfunction has been linked to cancer, heart, and brain disease. High-resolution structures of mammalian are not available. The sodium-bicarbonate cotransporter NBCe1 functions multiple organs its mutations cause blindness, abnormal growth blood chemistry, migraines, impaired cognitive function. Here, we have determined the structure membrane domain dimer at 3.9 Å resolution by cryo electron...
Elevated serum cholesterol levels play an important role in the development of coronary artery disease. Previous studies have suggested that nut consumption benefits lipid profile. Pistachio nuts are widely available, inexpensive and frequently consumed by general population.To determine whether substituting 20% daily caloric intake form pistachio will improve profiles humans with primary, moderate hypercholesterolemia.Controlled, randomized crossover design.Outpatient dietary modification,...
A dual-excitation inverted confocal laser-scanning microscope has been developed for measuring intracellular pH (pHi) using 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) in individual cells the isolated perfused cortical collecting tubule (CCT). This new superior depth discrimination, which eliminates contribution of fluorescence information from outside plane focus. pHi was monitored real time a spot 0.55 microns diameter within single cell. Experiments were performed to examine...
The interaction between late endocytic structures and microtubules in polarized cells was studied using a procedure previously shown to cause microtubule-dependent redistribution of lysosomes fibroblasts macrophages (Heuser, J. 1989. Cell Biol. 108:855-864). In cultured rat hippocampal neurons, low cytoplasmic pH caused cation-independent mannose-6-phosphate receptor-enriched move out the cell body into processes. filter grown MDCK lowering cytosolic approximately 6.5 endosomes base this...
The apical transport processes responsible for proton secretion were studied in the isolated perfused rabbit S3 proximal tubule. Intracellular pH (pHi) was measured with dye, 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. Steady state pHi tubules nominally HCO3(-)-free solutions 7.08 +/- 0.03. Removal of Na+ (lumen) caused a decrease 0.34 0.06 pH/min. inhibited 62% by 1 mM amiloride and unaffected 50 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid Cl- removal (lumen, bath). After...
The gene repertoire regulating vertebrate biomineralization is poorly understood. Dental enamel, the most highly mineralized tissue in mammals, differs from other calcifying systems that formative cells (ameloblasts) lack remodeling activity and largely degrade resorb initial extracellular matrix. Enamel mineralization requires ameloblasts undergo a profound functional switch matrix-secreting to maturational (calcium transport, protein resorption) roles as progresses. During maturation...
Transcellular bicarbonate transport is suspected to be an important pathway used by ameloblasts regulate extracellular pH and support crystal growth during enamel maturation. Proteins that play a role in amelogenesis include members of the ABC transporters (SLC gene family CFTR). A number carbonic anhydrases (CAs) have also been identified. The defined functions these genes are likely interlinked mineralization. purpose this study quantify relative mRNA levels individual SLC, Cftr, CAs cells...
The advent of cell culture-based methods for the establishment and expansion human corneal endothelial cells (CEnC) has provided a source transplantable endothelium, with significant potential to challenge one donor-one recipient paradigm. However, concerns over identity remain, comprehensive characterization cultured CEnC across serial passages not been performed. To this end, we compared two established culture by assessing transcriptomic changes that occur during in vitro expansion. In...
Abstract SLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) NBCe1 (SLC4A4) revealed an identical overall fold despite their different transport modes (chloride/bicarbonate exchange versus sodium-carbonate cotransport). However, exact mechanism determining family remains unknown. In this work, we report cryo-EM 3.4 Å structure OF NDCBE (SLC4A8), which shares...
Sodium-bicarbonate cotransporters are homologous membrane proteins mediating the electrogenic or electroneutral transport of sodium and bicarbonate. Of functionally characterized sodium-bicarbonate (NBC), NBC1proteins known to be electrogenic. Here we report cloning functional characterization NBC4c, a new splice variant NBC4 gene. At amino acid level, NBC4c is 56% identical NBC1 protein variants 40% NBC3. When expressed in mammalian cells, mediates cotransport. The bicarbonate chloride...
1 The pancreatic variant of the sodium bicarbonate cotransporter, pNBC1, mediates basolateral influx in exocrine pancreas by coupling transport to that sodium, with a 2 HCO3−:1 Na+ stoichiometry. kidney variant, kNBC1, efflux proximal tubule 3 HCO3− Na+. molecular basis underlying different stoichiometries is not known. pNBC1 and kNBC1 are 93 % identical each other 41 N-terminal amino acids replaced 85 distinct pNBC1. In this study we tested hypothesis differences stoichiometry related...