A5050582773- Renal Transplantation Outcomes and Treatments
- Transplantation: Methods and Outcomes
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- Organ Transplantation Techniques and Outcomes
KU Leuven
2022-2024
Abstract Rejection remains the main cause of premature graft loss after kidney transplantation, despite use potent immunosuppression. This highlights need to better understand composition and cell-to-cell interactions alloreactive inflammatory infiltrate. Here, we performed droplet-based single-cell RNA sequencing 35,152 transcriptomes from 16 transplant biopsies with varying phenotypes severities rejection without rejection, identified cell-type specific gene expression signatures for...
Solid phase detection and identification of HLA antibodies in kidney transplantation currently relies on single antigen bead (Luminex®) assays, which is more sensitive than the previously used enzyme‐linked immunosorbent assays (ELISA). To evaluate impact testing antibody‐mediated rejection (AMR) occurrence allograft survival, we analysed 1818 renal recipients transplanted between March 2004 May 2021. In 2008, solid switched from ELISA to Luminex. We included 393 (21.6%) transplantations...
Abstract Rejection remains the main cause of premature graft loss after kidney transplantation, despite use potent immunosuppression. This highlights need to better understand composition and interactions alloreactive inflammatory infiltrate. We performed droplet-based single-cell RNA sequencing 35,152 transcriptomes from 16 transplant biopsies generated cell-type specific gene expression signatures for deconvolution bulk tissue. A association was identified between recipient-derived FCGR3A+...