A5004444312- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Microfluidic and Bio-sensing Technologies
- Pancreatic and Hepatic Oncology Research
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
University of Glasgow
2023-2024
Centre for Human Genetics
2022
University of Oxford
2022
Intestinal homeostasis is underpinned by LGR5+ve crypt-base columnar stem cells (CBCs), but following injury, dedifferentiation results in the emergence of LGR5−ve regenerative cell populations (RSCs), characterized fetal transcriptional profiles. Neoplasia hijacks signaling, so we assessed distribution CBCs and RSCs mouse human intestinal tumors. Using combined molecular-morphological analysis, demonstrate variable expression markers across a range lesions. The degree CBC-RSC admixture was...
Abstract Inertial microfluidics has demonstrated tremendous potential to impact biological - and notably medical fields, by offering a highly versatile, portable cost-effective approach cell focusing sorting. While the range of applications inertial devices spans diagnostics, bioprocessing or water engineering mention few, translation is still impeded lack clear understanding interactions in such devices. This often leads bespoke designs that take years development characterisation for one...
Promastigote Leishmania mexicana have a complex cell division cycle characterised by the ordered replication of several single-copy organelles, prolonged S phase and rapid G2 cytokinesis phases, accompanied stage-associated morphological changes. Here we exploit these changes to develop high-throughput semi-automated imaging flow cytometry (IFC) pipeline analyse in live L. mexicana. Firstly, demonstrate that, unlike other DNA stains, Vybrant™ DyeCycle™ Orange (DCO) is non-toxic enables...
Abstract Promastigote Leishmania mexicana have a complex cell division cycle characterised by the ordered replication of several single-copy organelles, prolonged S phase and rapid G2 cytokinesis phases, accompanied stage-associated morphological changes. Here we exploit these changes to develop high-throughput semi-automated imaging flow cytometry (IFC) pipeline analyse L. in live cells. Firstly, demonstrate that, unlike other DNA stains, Vybrant™ DyeCycle™ Orange (DCO) is non-toxic enables...
Intestinal homeostasis is underpinned by LGR5+ve crypt-base columnar stem cells (CBCs), but following injury, dedifferentiation results in the emergence of LGR5-ve regenerative cell populations (RSCs), characterised fetal transcriptional profiles. Neoplasia hijacks signalling, so we assessed distribution CBCs and RSCs mouse human intestinal tumors. Using combined molecular-morphological analysis demonstrate variable expression markers across a range lesions. The degree CBC-RSC admixture was...