A5009546197- Polyomavirus and related diseases
- Full-Duplex Wireless Communications
- Plant Virus Research Studies
- Extracellular vesicles in disease
- Energy Harvesting in Wireless Networks
- Aquaculture Nutrition and Growth
- Multiple Sclerosis Research Studies
- Neonatal Respiratory Health Research
- Reproductive biology and impacts on aquatic species
- Congenital Diaphragmatic Hernia Studies
- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Antenna Design and Analysis
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Neurobiology and Insect Physiology Research
- Plant and fungal interactions
- Perovskite Materials and Applications
- Viral-associated cancers and disorders
- Cell death mechanisms and regulation
- MXene and MAX Phase Materials
- Endoplasmic Reticulum Stress and Disease
- Physiological and biochemical adaptations
- Ethics and Legal Issues in Pediatric Healthcare
- Plant Reproductive Biology
Brown University
2015-2025
Women & Infants Hospital of Rhode Island
2008
Providence College
2008
John Brown University
2001
JC polyomavirus (JCPyV) is a ubiquitous human pathogen that causes progressive multifocal leukoencephalopathy (PML), severe and often fatal neurodegenerative disease in immunocompromised or immunomodulated patients. The mechanisms responsible for initiating infection susceptible cells are not completely known. major attachment receptor the virus, lactoseries tetrasaccharide c (LSTc), paradoxically expressed on oligodendrocytes astrocytes brain, virus does bind to these cells. Because cell...
The human polyomavirus, JCPyV, is the causative agent of progressive multifocal leukoencephalopathy (PML) in immunosuppressed and immunomodulated patients. Initial infection with JCPyV common virus establishes a long-term persistent urogenital system 50–70% population worldwide. A major gap field that we do not know how traffics from periphery to brain cause disease. Our recent discovery choroid plexus epithelial cells are fully susceptible together reports vivo has led us hypothesize plays...
The human JC polyomavirus (JCPyV) causes the rapidly progressing demyelinating disease progressive multifocal leukoencephalopathy (PML). occurs most often in individuals with AIDS but also receiving immunomodulatory therapies for immune-related diseases such as multiple sclerosis. JCPyV infection of host cells requires pentasaccharide lactoseries tetrasaccharide c (LSTc) and serotonin receptor 5-hydroxytryptamine (5-HT) 5-HT2AR. While LSTc is involved initial attachment virus to via...
ABSTRACT JC Polyomavirus is the causative agent of progressive multifocal leukoencephalopathy (PML), an often-fatal demyelinating disease. Unfortunately, a diagnosis PML occurs only after patients have suffered irreversible neuropathologies. The first step in initiation viral entry to brain, but route and mechanisms responsible for neuroinvasion not been well established. To gain better understanding this, we asked whether purified virus or associated with extracellular vesicles (EVs) could...
JC virus (JCV) is a human polyomavirus and the causative agent of fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). JCV infection host cells dependent on interactions with cell surface asparagine (N)-linked sialic acids serotonin 5-hydroxytryptamine(2A) receptor (5-HT(2A)R). The 5-HT(2A)R contains five potential N-linked glycosylation sites extracellular N terminus. Glycosylation other receptors essential for expression, ligand binding, function. Also, cellular...
ABSTRACT JC polyomavirus (JCPyV) establishes a lifelong persistence in roughly half the human population worldwide. The cells and tissues that harbor persistent virus vivo are not known, but renal tubules other urogenital epithelial likely candidates as is shed urine of healthy individuals. In an immunosuppressed host, JCPyV can become reactivated cause progressive multifocal leukoencephalopathy (PML), fatal demyelinating disease central nervous system. Recent observations indicate may...
JC polyomavirus (JCPyV) is a ubiquitous human pathogen that causes progressive multifocal leukoencephalopathy (PML). The entry receptors for JCPyV belong to the 5-hydroxytryptamine 2 receptor (5-HT2R) family, but how individual members of family function facilitate infection not known. We used proximity ligation assay (PLA) determine interacts with each 5-HT2 (5-HT2Rs) in narrow window time during entry. CRISPR-Cas9 randomly introduce stop codons gene and discovered second intracellular loop...
Abstract JC polyomavirus (JCPyV) is a small, non‐enveloped virus that persists in the kidney about half adult population. In severely immune‐compromised individuals JCPyV causes neurodegenerative disease progressive multifocal leukoencephalopathy (PML) brain. has been shown to infect cells by both direct and indirect mechanisms, latter involving extracellular vesicle (EV) mediated infection. While mechanisms of infection are well studied EV poorly understood. Using combination chemical...
ABSTRACT The human polyomavirus, JCPyV, establishes a lifelong persistent infection in the peripheral organs of majority population worldwide. Patients who are immunocompromised due to underlying infections, cancer, or immunomodulatory treatments for autoimmune disease at risk developing progressive multifocal leukoencephalopathy (PML) when virus invades CNS and infects macroglial cells brain parenchyma. It is not yet known how enters cause disease. blood-choroid plexus barrier potential...
Cortical granules are specialized organelles whose contents interact with the extracellular matrix of fertilized egg to form block polyspermy. In sea urchins, granule a fertilization envelope (FE), and this construction is critically dependent upon protease activity. An autocatalytic serine protease, cortical 1 (CGSP1), has been identified in Strongylocentrotus purpuratus eggs, here we examined regulation activity tested potential target substrates CGSP1. We found that CGSP1 stored its...
Abstract Background Myocardial hypoxic-ischemic injury is the cause of significant morbidity and mortality worldwide. The cardiomyocyte response to known include changes in cell cycle regulators. cyclin-dependent kinase inhibitor p57 Kip 2 involved control, differentiation, stress signaling apoptosis. In contrast other inhibitors, Kip2 expression diminishes during postnatal life reactivated adult heart under conditions cardiac stress. Overexpression has been previously shown prevent...
The human polyomavirus JCPyV is an opportunistic pathogen that infects greater than 60% of the world's population. virus establishes a persistent and asymptomatic infection in urogenital system but can cause fatal demyelinating disease immunosuppressed or immunomodulated patients following invasion CNS. mechanisms responsible for into CNS tissues are not known direct from blood to cerebral spinal fluid via choroid plexus has been hypothesized. To study potential as site neuroinvasion, we...
Several classes of immunomodulators are used for treating relapsing-remitting multiple sclerosis (RRMS). Most these disease-modifying therapies, except teriflunomide, carry the risk progressive multifocal leukoencephalopathy (PML), a severely debilitating, often fatal virus-induced demyelinating disease. Because teriflunomide has been shown to have antiviral activity against DNA viruses, we investigated whether treatment cells with inhibits infection and spread JC polyomavirus (JCPyV),...
JC polyomavirus (JCPyV) is a ubiquitous, double-stranded DNA virus that causes the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML) in immunocompromised patients. Current treatments for PML are limited to immune reconstitution, and no effective antivirals exist. In this report, we show oxindole GW-5074 (3-(3,5-dibromo-4-hydroxybenzylidene)-5-iodoindolin-2-one) reduces JCPyV infection primary immortalized cells. This compound potently inhibits spread, which...