A5030764453- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Plant biochemistry and biosynthesis
- Bee Products Chemical Analysis
- Toxin Mechanisms and Immunotoxins
- Contact Dermatitis and Allergies
- Plant Reproductive Biology
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
- Biochemical and Molecular Research
- Microfluidic and Capillary Electrophoresis Applications
- Parasitic Infections and Diagnostics
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Innovative Microfluidic and Catalytic Techniques Innovation
Universität Innsbruck
2019-2024
Allergens are substances that cause abnormal immune responses and can originate from various sources. IgE-mediated allergies one of the most common severe types allergies, affecting more than 20% population in Western countries. be subdivided into a limited number families based on their structure, but this does not necessarily indicate origin or route administration allergen, nor is molecular basis allergenicity clearly understood. This review examines how understanding proteins involves...
PR-10 proteins constitute a major cause of food allergic reactions. Birch-pollen-related allergies are triggered by the immunologic cross-reactivity IgE antibodies with structurally homologous that present in birch pollen and various sources. While three-dimensional structures allergens have been characterized detail, only few experimental studies addressed structural flexibility these proteins. In this study, we analyze millisecond-timescale thirteen from prevalent plant sources NMR...
Additive manufacturing (3D printing) has greatly revolutionized the way researchers approach certain technical challenges. Despite its outstanding print quality and resolution, stereolithography (SLA) printing is cost-effective relatively accessible. However, applications involving mass spectrometry (MS) are few due to residual oligomers additives leaching from SLA-printed devices that interfere with MS analyses. We identified crosslinking agent urethane dimethacrylate as main contaminant...
A major proportion of allergic reactions to hazelnuts (Corylus avellana) are caused by immunologic cross-reactivity IgE antibodies pathogenesis-related class 10 (PR-10) proteins. Intriguingly, the four known isoforms hazelnut PR-10 allergen Cor a 1, denoted as 1.0401-Cor 1.0404, share sequence identities exceeding 97% but possess different properties. In this work we describe NMR solution structures these proteins and provide an in-depth study their biophysical Despite sharing highly similar...
The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. Here, we investigate the structural basis for recognition by proteins, through crystal structure domain Legionella pneumophila DNA uptake regulator, RocC, bound to transcriptional terminator its primary partner, sRNA RocR. reveals specific 3' nucleotide a conserved pocket involving β-turn-α-helix motif, while hairpin portion is recognized α-helical N-cap motif....
Abstract Here we report the efficient synthetic access to 13 C/ 15 N‐labelled pseudouridine phosphoramidites, which were incorporated into a binary H/ACA box guide RNA/product complex comprising 77 nucleotides (nts) in total and 75 nt E. coli tRNA Gly . The stable isotope (SI) labelled pseudouridines produced via highly chemo‐enzymatic synthesis. N uracils chemical synthesis enzymatically converted 5′‐monophosphate (ΨMP) by using YeiN, Ψ‐5′‐monophosphate C ‐glycosidase. Removal of...
Abstract In large parts of Europe, Northern America and China people are suffering from allergies after consuming certain kinds fruits vegetables. Typical allergic symptoms range scratching itching the throat to severe like rhino conjunctivitis anaphylaxis. For hazelnuts ( Corylus avellana ), these result initial sensitization birch Betula verrucosa ) pollen allergen Bet v 1 subsequent development cross-reactions proteins that similar in their three-dimensional structure sensitizing protein...
Abstract Kiwifruits have become one of the most common food sources triggering allergic reactions. In patients suffering from birch pollen related allergy, reactions result initial sensitization to ( Betula verrucosa ) allergen Bet v 1, followed by immunological cross-reactivity structurally homologous proteins in kiwifruit. Clinical symptoms range scratching and itching oral cavity more severe such as rhino conjunctivitis. this work we assigned backbone side chain 1 H, 13 C 15 N chemical...
Allergies related to kiwi consumption have become a growing health concern, with their prevalence on the rise. Many of these allergic reactions are attributed cross-reactivity, particularly major allergen found in birch pollen. This cross-reactivity is associated proteins belonging pathogenesis-related class 10 (PR-10) protein family. In our study, we determined three-dimensional structures two PR-10 gold and green fruits, Act c 8 d 8, using nuclear magnetic resonance (NMR) spectroscopy. The...
Abstract A major proportion of allergic reactions to hazelnuts ( Corylus avellana ) are caused by immunologic cross-reactivity IgE antibodies pathogenesis-related class 10 (PR-10) proteins. Intriguingly, the four known isoforms hazelnut PR-10 allergen Cor a 1, denoted as 1.0401-Cor 1.0404, share sequence identities exceeding 97 % but possess different properties. In this work we describe NMR solution structures these proteins and provide an in-depth study their biophysical Despite sharing...