A5039970877- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Nutrition, Genetics, and Disease
- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- Animal Genetics and Reproduction
- Bacterial Genetics and Biotechnology
- ATP Synthase and ATPases Research
- PARP inhibition in cancer therapy
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- FOXO transcription factor regulation
- Toxin Mechanisms and Immunotoxins
- Sirtuins and Resveratrol in Medicine
- Plant Molecular Biology Research
- Biochemical and Molecular Research
Pennsylvania State University
2023-2024
Institute for Bioscience and Biotechnology Research
2019
National Institute of Standards and Technology
2019
Sirtuin 6 (SIRT6) is a multifaceted protein deacetylase/deacylase and major target for small-molecule modulators of longevity cancer. In the context chromatin, SIRT6 removes acetyl groups from histone H3 in nucleosomes, but molecular basis its nucleosomal substrate preference unknown. Our cryo–electron microscopy structure human complex with nucleosome shows that catalytic domain pries DNA entry-exit site exposes N-terminal helix, while zinc-binding binds to acidic patch using an arginine...
The yeast SWR1C chromatin remodeling enzyme catalyzes the ATP-dependent exchange of nucleosomal histone H2A for variant H2A.Z, a key involved in multitude nuclear functions. How 14-subunit engages substrate remains largely unknown. Studies on ISWI, CHD1, and SWI/SNF families enzymes have demonstrated roles acidic patch activity, however role this epitope nucleosome editing by has not been tested. Here, we employ variety biochemical assays to demonstrate an essential H2A.Z reaction. Utilizing...
The yeast SWR1C chromatin remodeling enzyme catalyzes the ATP-dependent exchange of nucleosomal histone H2A for variant H2A.Z, a key involved in multitude nuclear functions. How 14-subunit engages substrate remains largely unknown. Studies on ISWI, CHD1, and SWI/SNF families enzymes have demonstrated roles acidic patch activity, however role this epitope nucleosome editing by has not been tested. Here, we employ variety biochemical assays to demonstrate an essential H2A.Z reaction. Utilizing...
The SWR1C chromatin remodeling enzyme catalyzes the ATP-dependent exchange of nucleosomal histone H2A for variant H2A.Z, a key involved in multitude nuclear functions. How 14-subunit engages substrate remains largely unknown. Studies on ISWI, CHD1, and SWI/SNF families enzymes have demonstrated roles acidic patch activity, however role this epitope nucleosome editing by has not been tested. Here, we employ variety biochemical assays to demonstrate an essential H2A.Z reaction. Utilizing...
Monoclonal antibodies (mAbs) represent an important platform for the development of biotherapeutic products. Most mAbs are produced in mammalian cells, but several made Escherichia coli, including therapeutic fragments. The NISTmAb is a well-characterized reference material widely available to facilitate both originator biologics and biosimilars. Here, when expressing from codon-optimized constructs E. coli (eNISTmAb), truncated variant its heavy chain was observed. N-terminal protein...
Abstract Despite the unique ability of pioneer transcription factors (PFs) to target nucleosomal sites in closed chromatin, they only bind a small fraction their genomic motifs. The underlying mechanism this selectivity is not well understood. Here, we design high-throughput assay called ChIP-ISO systematically dissect sequence features affecting binding specificity classic PF, FOXA1. Combining with vitro and neural network analyses, find that 1) FOXA1 strongly affected by co-binding TFs...
The SWR1C chromatin remodeling enzyme catalyzes the ATP-dependent exchange of nucleosomal histone H2A for variant H2A.Z, a key involved in multitude nuclear functions. How 14-subunit engages substrate remains largely unknown. Numerous studies on ISWI, CHD1, and SWI/SNF families enzymes have demonstrated roles acidic patch activity, however role this epitope nucleosome editing by has not been tested. Here, we employ variety biochemical assays to demonstrate an essential H2A.Z reaction....
ABSTRACT The SWR1C chromatin remodeling enzyme catalyzes the ATP-dependent exchange of nucleosomal histone H2A for variant H2A.Z, a key involved in multitude nuclear functions. How 14-subunit engages substrate remains largely unknown. Numerous studies on ISWI, CHD1, and SWI/SNF families enzymes have demonstrated roles acidic patch activity, however role this epitope nucleosome editing by has not been tested. Here, we employ variety biochemical assays to demonstrate an essential H2A.Z...