A5024478376- Enzyme Structure and Function
- Parasitic Infections and Diagnostics
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme-mediated dye degradation
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial bioremediation and biosurfactants
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Legionella and Acanthamoeba research
- Enzyme Catalysis and Immobilization
- Protein Structure and Dynamics
- Nuclear Physics and Applications
- Advanced Cellulose Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Algal biology and biofuel production
- Spectroscopy and Quantum Chemical Studies
- Microbial metabolism and enzyme function
- Viral Infections and Vectors
- Microbial Natural Products and Biosynthesis
- Biocrusts and Microbial Ecology
- Vector-borne infectious diseases
- RNA and protein synthesis mechanisms
National Institute of Standards and Technology
2018-2023
Institute for Bioscience and Biotechnology Research
2019-2023
Research Institute for Bioscience and Biotechnology
2023
North Carolina State University
2013-2022
Oak Ridge National Laboratory
2012-2022
Material Measurement Laboratory
2020-2022
National Institute of Standards
2021
University of Tennessee at Knoxville
2012
University of Nebraska–Lincoln
1985-1994
University of Nebraska at Omaha
1989
Abstract Lytic polysaccharide monooxygenases have attracted vast attention owing to their abilities disrupt glycosidic bonds via oxidation instead of hydrolysis and enhance enzymatic digestion recalcitrant substrates including chitin cellulose. We determined high‐resolution X‐ray crystal structures an enzyme from Neurospora crassa in the resting state a copper(II) dioxo intermediate complex formed absence substrate. also revealed “pre‐bound” molecular oxygen adjacent active site. An...
The scattering of neutrons can be used to provide information on the structure and dynamics biological systems multiple length time scales. Pursuant a National Science Foundation-funded workshop in February 2018, recent developments this field are reviewed here, as well future prospects that expected given advances sources, instrumentation computational power methods. Crystallography, solution scattering, dynamics, membranes, labeling imaging examined. For extraction maximum information,...
Metalloproteins perform a diverse array of redox-related reactions facilitated by the increased chemical functionality afforded their metallocofactors. Lytic polysaccharide monooxygenases (LPMOs) are class copper-dependent enzymes that responsible for breakdown recalcitrant polysaccharides via oxidative cleavage at glycosidic bond. The activated copper-oxygen intermediates and mechanism formation remains to be established. Neutron protein crystallography which permits direct visualization...
The structure of the disaccharide cellulose subunit cellobiose (4-O-β-D-glucopyranosyl-D-glucose) in solution has been determined via neutron diffraction with isotopic substitution (NDIS), computer modeling and nuclear magnetic resonance (NMR) spectroscopic studies. This study shows direct evidence for an intramolecular hydrogen bond between reducing ring HO3 hydroxyl group non-reducing oxygen (O5') that previously predicted by computation NMR analysis. Moreover, this work bonding to O5' is...
The arrangement of water and chloride ions around a model peptide (glycyl-L-prolyl-glycine-NH2) was investigated using Molecular Dynamics (MD) simulations complementary Empirical Potential Structure Refinement (EPSR) which adapt the modelled structure to reproduce experimentally measured neutron diffraction data. results are in good qualitative agreement show common picture for all hydrogen-containing amine amide groups: namely that there two interactions observed – direct contact between...
Lytic polysaccharide monooxygenases (LPMOs) are carbohydrate-disrupting enzymes secreted by bacteria and fungi that break glycosidic bonds via an oxidative mechanism. Fungal LPMOs typically act on cellulose can enhance the efficiency of cellulose-hydrolyzing release soluble sugars for bioethanol production or other industrial uses. The enzyme PMO-2 from Neurospora crassa (NcPMO-2) was heterologously expressed in Pichia pastoris to facilitate crystallographic studies fungal LPMO Diffraction...
Abstract Lytic polysaccharide monooxygenases have attracted vast attention owing to their abilities disrupt glycosidic bonds via oxidation instead of hydrolysis and enhance enzymatic digestion recalcitrant substrates including chitin cellulose. We determined high‐resolution X‐ray crystal structures an enzyme from Neurospora crassa in the resting state a copper(II) dioxo intermediate complex formed absence substrate. also revealed “pre‐bound” molecular oxygen adjacent active site. An...
The chlorosome is a highly specialized supramolecular light-harvesting antenna complex found in green photosynthetic bacteria and composed of self-assembled bacteriochlorophyll (BChl) pigments entrapped lipid vesicle. These organelles are interest for development synthetic devices solar harvesting conversion because the organization packing BChls provides efficient light collection energy funneling mechanism with properties that superior to similar artificial systems based on BChl pigment...
Monoclonal antibodies (mAbs) represent an important platform for the development of biotherapeutic products. Most mAbs are produced in mammalian cells, but several made Escherichia coli, including therapeutic fragments. The NISTmAb is a well-characterized reference material widely available to facilitate both originator biologics and biosimilars. Here, when expressing from codon-optimized constructs E. coli (eNISTmAb), truncated variant its heavy chain was observed. N-terminal protein...
Revealing the positions of all atoms in large macromolecules is powerful but only possible with neutron macromolecular crystallography (NMC). Neutrons provide a sensitive and gentle probe for direct detection protonation states at near-physiological temperatures clean artifacts caused by x rays or electrons. Currently, NMC use restricted requirement crystal volumes even state-of-the-art instruments such as diffractometer Spallation Neutron Source. EWALD's design will break volume barrier...
Labeling of proteins with deuterium is an essential tool in overcoming size limitations the application nuclear magnetic resonance (NMR) spectroscopy to larger than 30 kilodaltons (kDa). A non-originator antigen-binding fragment (Fab) NIST RM 8671 NISTmAb, so called yNIST-Fab, a ~ 50 kDa protein, 5 native disulfide linkages, that can be expressed properly folded form methylotrophic Komagataella phaffii (formerly Pichia pastoris ). Further, K. host support production perdeuterated yNIST-Fab...
Lytic polysaccharide monooxygenases (LPMOs) are copper-center enzymes that involved in the oxidative cleavage of glycosidic bond crystalline cellulose and other polysaccharides. The LPMO reaction is initiated by addition a reductant oxygen to ultimately form an unknown activated copper–oxygen species responsible for polysaccharide-substrate H-atom abstraction. Given sensitivity metalloproteins radiation damage, neutron protein crystallography provides nondestructive technique structural...
The objectives of this study are to document the effect parasitism on vole populations and investigate small mammals Grand Teton National Park as potential reservoirs human parasites. Immediate goals for year were (1) continue documentation incidence prevalence parasites these mammals; (2) determine age at which Giardia infections contracted by Microtus host; (3) identify ticks associated with mammal populations; (4) survey animals Babesia infections.
Die oxidative Spaltung glykosidischer Bindungen ermöglicht es lytischen Polysaccharid-Monooxygenasen (LPMOs), die Polysaccharidketten aufzubrechen. Diese Aktivität verstärkt enzymatische Hydrolyse von hartnäckiger Kohlenhydrat-Biomasse wie Zellulose oder Chitin. F. Meilleur und Mitarbeiter liefern in ihrer Zuschrift auf S. 785 eine strukturelle Beschreibung der Sauerstoffaktivierung am einkernigen Kupferzentrum einer LPMO Grundlage Röntgen- Neutronenkristallographie sowie DFT-Rechnungen....