A5057223751- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Protein purification and stability
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Advanced NMR Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Electron Spin Resonance Studies
- Receptor Mechanisms and Signaling
- HIV/AIDS drug development and treatment
- RNA Research and Splicing
- Enzyme Structure and Function
- NMR spectroscopy and applications
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Chromatography
- Sulfur Compounds in Biology
- Mass Spectrometry Techniques and Applications
- Bacterial Genetics and Biotechnology
- Cytomegalovirus and herpesvirus research
- Biochemical and Molecular Research
- Neuroscience and Neuropharmacology Research
- Redox biology and oxidative stress
Institute for Bioscience and Biotechnology Research
2012-2025
National Institute of Standards and Technology
2016-2025
Advanced Bioscience Laboratories (United States)
2002-2025
Research Institute for Bioscience and Biotechnology
2023
Material Measurement Laboratory
2013-2017
National Institute of Standards
2017
Bristol-Myers Squibb (United States)
2015
University Hospitals of Cleveland
2013
Biotechnology Institute
2000-2009
University of Maryland, Baltimore County
2008
Highly fluorescent nucleotide base analogs provide sensitive probes for studying the structure, dynamics and interactions of nucleic acids. These can be incorporated site-specifically into oligonucleotides through standard automated synthetic methods, allowing them to serve as changes in microenvironment bases that may result from variation buffer condition, ion concentration, temperature or molecular interactions. Global conformational acids acid complexes also detected using analogs. A...
The increased interest in using monoclonal antibodies (mAbs) as a platform for biopharmaceuticals has led to the need new analytical techniques that can precisely assess physicochemical properties of these large and very complex drugs purpose correctly identifying quality attributes (QA). One QA, higher order structure (HOS), is unique essential establishing consistency biopharmaceutical manufacturing, detecting process-related variations from manufacturing changes comparability between...
The initial interaction between the ColE1 plasmid specific transcripts RNA I and II, which function as antisense regulators of replication, comprises a transient complex complementary loops found within secondary structures. Multidimensional heteronuclear magnetic resonance spectroscopy was used to characterize complexes formed model hairpins having seven nucleotide loops. Seven base pairs are in loop-loop helix, with continuous helical stacking loop residues on 3' side their stems. A sharp...
Two-dimensional (2D)
The scattering of neutrons can be used to provide information on the structure and dynamics biological systems multiple length time scales. Pursuant a National Science Foundation-funded workshop in February 2018, recent developments this field are reviewed here, as well future prospects that expected given advances sources, instrumentation computational power methods. Crystallography, solution scattering, dynamics, membranes, labeling imaging examined. For extraction maximum information,...
Rev is an essential HIV-1 regulatory protein that binds the responsive element (RRE) within env gene of RNA genome and involved in transport unspliced or partially spliced viral mRNA from cell nucleus to cytoplasm. Previous studies have shown a short α-helical peptide derived (Rev 34−50), truncated form RRE sequence provide useful vitro system study this interaction while still preserving aspects native complex. We selectively incorporated fluorescent probe 2-aminopurine 2'-O-methylriboside...
Abstract The ongoing COVID-19 pandemic highlights the necessity for a more fundamental understanding of coronavirus life cycle. causative agent disease, SARS-CoV-2, is being studied extensively from structural standpoint in order to gain insight into key molecular mechanisms required its survival. Contained within untranslated regions SARS-CoV-2 genome are various conserved stem-loop elements that believed function RNA replication, viral protein translation, and discontinuous transcription....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThree-Dimensional Triple-Resonance 1H, 13C, 31P Experiment: Sequential Through-Bond Correlation of Ribose Protons and Intervening Phosphorus along the RNA Oligonucleotide BackboneJohn P. Marino, Harald Schwalbe, Clemens Anklin, Wolfgang Bermel, Donald M. Crothers, Christian GriesingerCite this: J. Am. Chem. Soc. 1994, 116, 14, 6472–6473Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July...
We report a comprehensive study of case aggressive natural killer cell lymphoma/leukemia, which is characterized by young male predominance, rapidly progressive clinical course, and presence lymphadenopathy, hepatosplenomegaly, bone marrow involvement. The leukemic phase frequently preceded pancytopenia. diagnostic clues are the detection cytoplasmic granules in tumor cells on Wright-Giemsa-stained tissue imprints or smears selective loss T-cell antigens. Immunophenotyping decisive making...
Rev is an essential regulatory HIV-1 protein that binds the responsive element (RRE) within env gene of RNA genome, activating switch between viral latency and active replication. Previously, we have shown selective incorporation fluorescent probe 2-aminopurine (2-AP) into a truncated form RRE sequence (RRE-IIB) allowed binding arginine-rich peptide derived from aminoglycosides to be characterized directly by fluorescence methods. Using these nuclear magnetic resonance (NMR) methods,...
NISTCHO is a Chinese hamster ovary (CHO) cell line expressing the same amino acid sequences as heavy and light chains of National Institute Standards Technology (NIST) monoclonal antibody [Reference Material (RM) 8671 NISTmAb]. was generated by MilliporeSigma to be developed NIST RM support biomanufacturing research innovation, method development qualification, pre-competitive collaboration. The line, denoted 8675 NISTCHO, Clonal CHO-K1 Cell Line Producing cNISTmAb, interest...
One-bond 1H,13C and geminal 1H,1H dipolar interactions are normally the dominant causes of 1H 13C transverse relaxation in NMR experiments applied to 13C,15N-labeled RNA solution. Proton,carbon multiple-quantum coherences, where all heteronuclei connected by single bonds evolved simultaneously plane, however, not affected these strong interactions. Consequently, lifetimes, or T2 values, resonances can be dramatically extended. Here, we show that pulse sequences written take advantage this...
Dimerization of two homologous strands genomic RNA is an essential feature retroviral replication. In the human immunodeficiency virus type 1 (HIV-1), a conserved stem-loop sequence, dimerization initiation site (DIS), has been identified as domain primarily responsible for this aspect viral assembly. The DIS loop contains autocomplementary hexanucleotide sequence flanked by highly 5' and 3' purines can form homodimer through loop-loop kissing interaction. structural rearrangement activated...
Replication of human immunodeficiency virus type 1 (HIV-1) is regulated in part through an interaction between the virally encoded trans -activator protein Tat and responsive region (TAR) viral RNA genome. Because TAR highly conserved its with required for efficient replication, it has received much attention as antiviral drug target. Here, we report a 2-aminopurine (2-AP) fluorescence-based assay evaluating potential inhibitors. Through selective incorporation 2-AP within bulge (C23 or U24)...