A5063792730- Research on Leishmaniasis Studies
- Immune cells in cancer
- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Complement system in diseases
- T-cell and B-cell Immunology
- Parasites and Host Interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Immunotherapy and Immune Responses
- Infectious Disease Case Reports and Treatments
- Reproductive System and Pregnancy
- Toxin Mechanisms and Immunotoxins
- Infections and bacterial resistance
- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Eosinophilic Disorders and Syndromes
- Inflammasome and immune disorders
- Adenosine and Purinergic Signaling
- Autoimmune and Inflammatory Disorders Research
- Glycosylation and Glycoproteins Research
- NF-κB Signaling Pathways
- Streptococcal Infections and Treatments
University of Maryland, College Park
2015-2024
University of Maryland, Baltimore
2022
Biogem
2009-2010
University of Mary
2010
Temple University
1993-2002
W.E. Upjohn Institute for Employment Research
2000
University of Pennsylvania
2000
Bayer (United States)
1998-2000
Society for Leukocyte Biology
1997-2000
Bayer (Netherlands)
1999
Abstract Macrophages are mononuclear phagocytes that widely distributed throughout the body. These cells can contribute to development and homeostasis participate in innate adaptive immune responses. The physiology of macrophages vary tremendously depending on environment which they reside local stimuli exposed. prodigious secretory cells, role promote regulate responses autoimmune pathologies. highly phagocytic, this capacity have long been considered be essential effector cells. important...
Abstract We generated three populations of macrophages (Mφ) in vitro and characterized each. Classically activated Mφ (Ca-Mφ) were primed with IFN-γ stimulated LPS. Type II-activated (Mφ-II) similarly but LPS plus immune complexes. Alternatively (AA-Mφ) overnight IL-4. Here, we present a side-by-side comparison the cell types. focus primarily on differences between Mφ-II AA-Mφ, as both have been classified M2 Mφ, distinct from Ca-Mφ. show that more closely resemble Ca-Mφ than they are to...
Abstract To determine the role of IL-10 in cutaneous leishmaniasis, we examined lesion development following Leishmania major infection genetically susceptible BALB/c mice lacking IL-10. Whereas normal developed progressive nonhealing lesions with numerous parasites within them, IL-10−/− controlled disease progression, and had relatively small 1000-fold fewer them by fifth week infection. We also a mechanism whereby induced production from macrophages. show that surface IgG on amastigotes...
NF-kappaB/Rel is a family of transcription factors whose activation has long been linked to the production inflammatory cytokines. Here, we studied NF-kappaB signaling in regulation anti-inflammatory cytokine, interleukin-10 (IL-10). We identified role for single member, NF-kappaB1 (p50), promoting IL-10. The ciselement on IL-10 proximal promoter was located -55/-46, where p50 can homodimerize and form complex with transcriptional co-activator CREB-binding protein activate transcription....
Interleukin (IL)-12 is a monocyte- and macrophage-derived cytokine that plays crucial role in both the innate acquired immune response. In this study, we examined effects ligating specific macrophage receptors had on induction of IL-12 by lipopolysaccharide (LPS). We report ligation Fcγ, complement, or scavenger inhibited LPS. Both mRNA synthesis protein secretion were diminished to near-undetectable levels following receptor ligation. Suppression was since IL-10 tumor necrosis factor-α...
Macrophages can respond to a variety of infectious and/or inflammatory stimuli by secreting an array proinflammatory cytokines, the overproduction which result in shock or even death. In this report, we demonstrate that ligation macrophage Fcγ receptors (FcγR) lead reversal responses inducing upregulation interleukin (IL)-10, with reciprocal inhibition IL-12 production. IL-10 was specific FcγR ligation, since Mac-1 receptor did not alter The identification subtype responsible for determined...
Activated macrophages were used as antigen presenting cells (APCs) to determine the extent which these APCs could influence an adaptive immune response. We show that activated induced a strong polarized Th1-like T cell response was predominated by IFN-gamma. However, when targeted Fcgamma receptors on macrophages, their phenotype changed, and they now IL-4. The initial biasing toward result of activation innate response, from MyD88(-/-) mice failed produce Th1-inducing cytokines. reversal...
Abstract The Leishmania surface protease gp63 has been identified as a parasite virulence factor. To better define the role of in infectivity, interaction recombinant with complement and receptors was examined. On Leishmania, not necessary for fixation. Complement activation occurred on transfected organisms expressing varying amounts mutant form devoid proteolytic activity. However, wild-type their fixed only small terminal components were dramatically more resistant to lysis by than those...
Abstract Our laboratory has previously demonstrated that the ligation of phagocytic receptors on macrophages can influence cytokine production. In this study, we examine responses to multiple inflammatory stimuli following FcγR ligation. Macrophages were stimulated in vitro with LPS, lipoteichoic acid, CD40 ligand, or low molecular mass hyaluronic acid. All these proinflammatory character, inducing production high levels IL-12, but only modest amounts IL-10. The coligation along resulted an...
We examined the role of immunoglobulin (Ig)G antibodies in mediating host defense to intracellular parasite, Leishmania. show that IgG not only fails provide protection against this pathogen, but it actually contributes disease progression. The JH strain BALB/c mice, which lack because they have a targeted deletion Ig heavy chain (J) locus, were more resistant infection with Leishmania major than normal mice. However, passive administration anti-Leishmania caused mice develop large lesions...
We examined the role of macrophage receptor for C3bi, CR3, in phagocytosis Leishmania major promastigotes and report that M1/70, a monoclonal antibody to inhibited binding leishmania macrophages both when assays were performed presence normal serum its absence. In serum, activate complement fix C3. Fixation subsequent cleavage C3bi occurs rapidly, by as early 5 min forms molecule can be identified on parasites' surface. Complement fixation results an enhanced mouse macrophages. case L....
Candida albicans exhibits hemolytic activity when grown on glucose-enriched blood agar. This is present intact organisms, and it secreted into the culture medium. Hemoglobin released from lysed erythrocytes can restore transferrin-inhibited growth of C. albicans. We conclude that expresses a factor which allows to acquire iron host erythrocytes.
Abstract Our understanding of cell mediated immunity (CMI) has revealed the importance activated macrophages as key immune effector cells. Over past decade, we have come to realize that exhibit remarkable plasticity, and different populations with distinct physiologies can develop in response stimuli. In fact, it is likely number macrophage arise may be diverse activating stimuli induce them. Some these instruct kill microbes (classical activation), lay down extracellular matrix components...
ABSTRACT Rhodococcus equi is a facultative intracellular pathogen of macrophages and cause pneumonia in young horses (foals) immunocompromised people. Isolates R. from pneumonic foals typically contain large, 85- or 90-kb plasmids encoding highly immunogenic virulence-associated protein (VapA). The objective this study was to determine the role 85-kb plasmid VapA survival virulence . Clinical isolates containing expressing efficiently replicated within mouse vitro, while plasmid-cured...