A5100337269- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Heat shock proteins research
- Ferroptosis and cancer prognosis
- Gut microbiota and health
- Metabolism and Genetic Disorders
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Gastrointestinal motility and disorders
- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Nuclear Structure and Function
- Genomics, phytochemicals, and oxidative stress
- Growth Hormone and Insulin-like Growth Factors
- Helicobacter pylori-related gastroenterology studies
- Autophagy in Disease and Therapy
- RNA regulation and disease
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
Peking University
2025
Nanjing Agricultural University
2024
General Hospital of Central Theater Command
2023
Central Hospital of Wuhan
2023
Zhongnan Hospital of Wuhan University
2022
Wuhan University
2022
First People's Hospital of Yuhang District
2021
Markey Cancer Center
2020
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2019-2020
The Ohio State University
2019-2020
It is largely recognized that PDCD4 frequently lost in tumors of various origins, including lung cancer, and its loss contributes to tumor progression. However, role molecular mechanism remain unexplored non-small cell cancer (NSCLC). In this study, downregulated mRNA expression was found NSCLC tissues compared their corresponding paracarcinoma distal tissues. Induced inhibited growth proliferation cycle transition vitro. Conversely, knocking down promoted proliferation. Mechanistically,...
A prerequisite for proteins to interact in a cell is that they are present the same intracellular compartment. Although it generally accepted proteasomes occur both, cytoplasm and nucleus, research has been focusing on cytoplasmic protein breakdown antigen processing, respectively. Thus, little known functional organization of proteasome nucleus. Here we report within nucleus 20S 26S throughout nucleoplasm partially colocalize with splicing factor–containing speckles. Because absent from...
Abstract N-acetyltransferase 10 (NAT10) is involved in regulating senescence. However, its role glomerular diseases remains unclear. Therefore, this study aims to investigate the mechanisms by which NAT10 influences senescence and damage an adriamycin (ADR)-induced nephropathy model. Senescence (p16 p21) DNA markers (γ-H2AX (ser139)) were assessed ADR-induced nephropathy. function was demonstrated using Remodelin or small interfering RNA (siRNA) interventions. Transcriptome sequencing...
Circular RNAs (circRNAs) are characterized as a class of new noncoding and function in tumorigenesis colorectal cancer (CRC). In our study, the molecule mechanism circ_0022340 CRC was investigated. For this aim, quantitative real-time polymerase chain reaction (RT-qPCR) used to test gene expression cells. Cell assays including 5-ethynyl-20-deoxyuridine (EdU), colony formation transwell investigated proliferation migration capacity Luciferase reporter RNA immunoprecipitation (RIP)assays...
Pancreatic cancer (PC) is one of the deadliest malignancies. Partner NOB1 homolog (PNO1) has been reported to be involved in tumorigenesis. However, role PNO1 PC remains elucidated. The purpose this study was examine effects on progression and possible mechanism related E2F transcription factor 1 (E2F1), a predicted by JASPAR database bind promoter region promoted proliferation pancreatic ductal adenocarcinoma. First, expression tissues its association with survival rate were analyzed Gene...
Abstract Deposition of ubiquitinated protein aggregates is a hallmark neurodegeneration in both acute neural injuries, such as stroke, and chronic conditions, Parkinson's disease, but the underlying mechanisms are poorly understood. In present study, we examined role Zn2+ ischemia-induced impairment ubiquitin-proteasome system CA1 region rat hippocampus after transient global ischemia. We found that scavenging endogenous reduced ubiquitin conjugation free depletion. Furthermore, exposure to...
The lack of tools to reliably detect RanBP9 in vivo has significantly hampered progress understanding the biological functions this scaffold protein. We report here generation a novel mouse strain, RanBP9-TT, which endogenous protein is fused with double (V5-HA) epitope tag at C-terminus. show that does not interfere essential RanBP9. In contrast constitutive knock-out animals, RanBP9-TT mice are viable, fertile and do any obvious phenotype. V5-HA allows unequivocal detection both by IHC WB....
Aerobic glycolysis is essential for cancer cell metabolism and growth. Deubiquitinase, USP28 (ubiquitin specific peptidase 28), could maintain stability of proteins involved in tumor progression. This study was performed to investigate the role aerobic colorectal cancer. Our data showed that mRNA protein expressions were enhanced tissues cells. Functional assays demonstrated overexpression promoted proliferation cancer, while inhibition reverse these effects. Protein expression Forkhead Box...
This study aimed to evaluate the value of chitinase activity in prognosticating occurrence metastasis and prognosis patients with colorectal cancer (CRC).The four different groups, namely 335 CRC without distant at their first visit (Group 1), 51 having synchronous liver 2), 100 healthy age-matched controls 3) 40 4), were assayed using an enzyme-linked immunosorbent assay. The Cox proportional hazards ratio model Kaplan-Meier curve used identify association between clinical outcome training...
Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous orthotopic xenografts have been developed to study metastatic process. By using lung line A549 as an example, we present a modified protocol establish xenograft. Our ensures sufficient establishment allows us monitor tumor growth spontaneous metastasis. This could be adapted other types established lines or primary cells mechanism process well test...
Abstract The lack of tools to reliably detect RanBP9 in vivo has significantly hampered progress understanding the biological functions this scaffold protein. We report here generation a novel mouse strain, RanBP9-TT, which endogenous protein is fused with double (V5-HA) epitope tag at C-terminus. show that does not interfere essential RanBP9. In contrast constitutive knock-out animals, RanBP9-TT mice are viable, fertile and do any obvious phenotype. V5-HA allows unequivocal detection both...
Abstract Targeting the lineage marker CLDN18.2 with ADCC-eliciting monoclonal antibody therapeutics has shown promising clinical activity in gastric, esophageal, and pancreatic cancer indications. However, efficacy was mainly observed tumors that expressed very high levels of CLDN18.2, which represented only ~30% cases, much less pronounced low CLDN18.2. Here we present ZL-1211, a novel CLDN18.2-targeting engineered to promote enhanced ADCC drive exhibit broad range expression levels. We...