A5072974009- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- Cancer-related Molecular Pathways
- interferon and immune responses
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
- Infectious Aortic and Vascular Conditions
- Nanoparticle-Based Drug Delivery
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Genomics, phytochemicals, and oxidative stress
- Renal and Vascular Pathologies
- Genomics and Chromatin Dynamics
- Drug Transport and Resistance Mechanisms
- CAR-T cell therapy research
- Renal Transplantation Outcomes and Treatments
- Advanced Electron Microscopy Techniques and Applications
The Ohio State University
2015-2025
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2018-2024
The Ohio State University Wexner Medical Center
2014-2024
Columbus Oncology and Hematology Associates
2023
Aristotle University of Thessaloniki
2022
Mediterranean Agronomic Institute of Chania
2008
Centre for Research and Technology Hellas
2008
Significance We discovered that IFITM3 prevents efficient dissemination and replication of influenza virus in heart tissue, thereby limiting cardiac fibrosis electrical dysfunction during infection. Since polymorphisms are among the only human genetic factors have been reproducibly associated with hospitalization mortality infection, our findings relevant to serious threat infection health. Furthermore, KO mice provide one first models for studying complications influenza.
Abstract Background The HECT E3 ubiquitin ligase Nedd4 has been shown to positively regulate T cell responses, but its role in helper (Th) differentiation and autoimmunity is unknown. Th17 cells are believed play a pivotal the development pathogenesis of autoimmune diseases. Nevertheless, regulation RORγt activation during by TCR signaling yet be elucidated. These uncharted aspects inspire us explore potential Th17-mediated autoimmunity. Methods We evaluated impact deficiency on mouse using...
Two-pore K(+) channels have emerged as potential targets to selectively regulate cardiac cell membrane excitability; however, lack of specific inhibitors and relevant animal models has impeded the effort understand role 2-pore in heart their a therapeutic target. The objective this study was determine mechanosensitive channel family member TREK-1 control excitability.Cardiac-specific TREK-1-deficient mice (αMHC-Kcnk(f/f)) were generated found prevalent sinoatrial phenotype characterized by...
INTRODUCTION: The blood brain barrier (BBB) is prohibitive to intravascular drug delivery and has restricted spinal cord of therapies intrathecal approaches. METHODS: A dumbbell shaped, retrievable infusion stentgraft (RDIS) was constructed from nitinol polytetrafluoroethylene. Seven anesthetized pigs underwent percutaneous femoral insertion the sheathed RDIS deployment in lower thoracic aorta by sheath withdrawal. Anti-glial fibrillary acidic protein (GFAP) antibody instilled outer chamber...
The biological functions of the scaffold protein Ran Binding Protein 9 (RanBP9) remain elusive in macrophages or any other cell type where this is expressed together with its CTLH (C-terminal to LisH) complex partners. We have engineered a new mouse model, named RanBP9-TurnX, RanBP9 fused three copies HA tag (RanBP9-3xHA) can be turned into RanBP9-V5 tagged upon Cre-mediated recombination. created model enable stringent biochemical studies at specific level throughout entire organism. Here,...
// Dario Palmieri 1, 2 , Mario Scarpa Anna Tessari 1 Rexhep Uka Foued Amari Cindy Lee Timothy Richmond Claudia Foray Tyler Sheetz Ashley Braddom Christin E. Burd Jeffrey D. Parvin Thomas Ludwig Carlo M. Croce Vincenzo Coppola Department of Molecular Virology, Immunology and Medical Genetics, College Medicine, 43210 Columbus, OH, USA Solid Tumor Biology Program, Comprehensive Cancer Center, The Ohio State University, Correspondence to: Coppola, e-mail: Vincenzo.coppola@osumc.edu Keywords:...
Brain somatic variants in SLC35A2 were recently identified as a genetic marker for mild malformations of cortical development with oligodendroglial hyperplasia epilepsy (MOGHE). The role and the contributions abnormal neurons oligodendrocytes to seizure activity MOGHE remain largely unexplored.
To assess the capacity of small molecules to function as antioxidants in pathologic conditions, a set yeast assays utilizing strains deficient antioxidant machinery was applied with measurements reactive oxygen species (ROS), glutathione (GSH/GSSG), and induction stress responsive proteins oye2 oye3. Yeast superoxide dismutase (Δsod1), catalase A (Δcta1), double-deficient Old Yellow enzyme 2 reductase 1 (Δoye2 glr1) were supplemented ascorbic acid, β-carotene, caffeic or quercetin, subjected...
IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at organismal level, we performed a systematic tissue analysis its expression and generated Iws1-deficient mice. A thorough immunohistochemical characterization shows that present in nucleus all cells most examined tissues, with few notable exceptions. We also report ablation Iws1 consistently causes lethality...
The lack of tools to reliably detect RanBP9 in vivo has significantly hampered progress understanding the biological functions this scaffold protein. We report here generation a novel mouse strain, RanBP9-TT, which endogenous protein is fused with double (V5-HA) epitope tag at C-terminus. show that does not interfere essential RanBP9. In contrast constitutive knock-out animals, RanBP9-TT mice are viable, fertile and do any obvious phenotype. V5-HA allows unequivocal detection both by IHC WB....
Concentrative nucleoside transporters (CNTs) are active influx systems, but their in vivo roles poorly defined. By generating CNT1 knockout (KO) mice, here we identify a role of the renal reabsorption nucleosides. Deletion mice increases urinary excretion endogenous pyrimidine nucleosides with compensatory alterations purine metabolism. In addition, KO exhibits high analog gemcitabine (dFdC), which results poor tumor growth control harboring syngeneic pancreatic tumors. Interestingly,...
Abstract Using an efficient gene targeting approach, we developed a novel mouse line that expresses the tetracycline-controlled transactivator (tTA) from constitutively active Eef1a1 locus in Cre recombinase-inducible manner. The temporally and spatially controlled expression of EF1-LSL-tTA knockin activation tTA-driven responder transgenes was tested using four transgenic lines express under tissue-specific promoters pancreas, mammary gland other secretory tissues, as well...
The biological functions of the scaffold protein Ran Binding Protein 9 (RanBP9) remain elusive in macrophages or any other cell type where this is expressed together with its CTLH (C-terminal to LisH) complex partners. We have engineered a new mouse model, named RanBP9-TurnX, RanBP9 fused three copies HA tag (RanBP9-3xHA) can be turned into RanBP9-V5 tagged upon Cre-mediated recombination. created model enable stringent biochemical studies at specific level throughout entire organism. Here,...
ObjectiveAneurysm pathophysiology remains poorly understood, in part from the disparity of murine models with human physiology and requirement for invasive aortic exposure to apply agents used create aneurysm models. A retrievable drug infusion stent graft (RDIS) was developed isolate wall intraluminally exposure. We hypothesized that an RDIS could deliver aneurysm-promoting enzymes a porcine model thoracic aneurysms without major surgical exposure.MethodsRetrievable nitinol frames were...
Abstract Influenza virus primarily targets the lungs, but dissemination and damage to heart tissue is also known occur in severe infections. Despite this knowledge, influenza virus-induced cardiac pathogenesis its underlying mechanisms have been difficult study due a lack of small animal models. In humans, polymorphisms gene encoding interferon-induced transmembrane protein 3 (IFITM3), an antiviral restriction factor, are associated with susceptibility influenza, whether IFITM3 deficiencies...
Abstract Currently, most of the anti-neoplastic treatments for lung cancer patients are based on DNA-damaging cytotoxic therapies such as platinum-containing compounds and radiotherapy. Therefore, a better understanding molecular mechanisms involved in cellular response to genotoxic stresses is required order improve clinical management cancer. Ran Binding Protein 9 (RanBP9, also known RanBP-M) ubiquitous evolutionary conserved scaffold protein that shuttles between nucleus cytoplasm,...
Objectives: Murine models present limitations for the study of aortic aneurysm due to disparate hemodynamics from humans, small size and necessity open surgery clamping apply otherwise toxic aneurysmogenic enzymes. We have developed a retrievable chambered stentgraft that can isolate wall more importantly restrict enzyme only anterior aorta. Figure 1 shows posterior central lumen (CL) preserves distal perfusion while excluding spinal cord branches, an outer chamber (OC) delivers drug wall....
Introduction: Half of vascular interventions will fail in under 5 years due to restenosis. While drug coated balloons (DCB) are used minimize restenosis, there several disadvantages: 1) Limitations the amount that can be adhered a balloon, 2) Significant is washed off balloon by circulation and 3) There limits on time inflation ischemia concerns. We developed dumbbell shaped, retrievable delivery stentgraft (RDDS) isolates segment artery with an outer chamber ( OC ) (Fig 1A). The center...