A5081909687- Renal Diseases and Glomerulopathies
- Hormonal Regulation and Hypertension
- Adenosine and Purinergic Signaling
- Chronic Kidney Disease and Diabetes
- Lipid metabolism and disorders
- Pancreatitis Pathology and Treatment
- Drug Transport and Resistance Mechanisms
- Caveolin-1 and cellular processes
- Urban and Rural Development Challenges
- Ovarian cancer diagnosis and treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Liver Disease Diagnosis and Treatment
- Asthma and respiratory diseases
- Renal and related cancers
- Tuberous Sclerosis Complex Research
- Renal Transplantation Outcomes and Treatments
- Peroxisome Proliferator-Activated Receptors
- Community Development and Social Impact
- Diabetes Treatment and Management
- Electrolyte and hormonal disorders
- African cultural and philosophical studies
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Influenza Virus Research Studies
Nationwide Children's Hospital
2022-2023
University of Cambridge
2020
Background: Glomerular disease, characterized by podocyte injury and proteinuria, can lead to CKD end-stage kidney disease. We hypothesized that the glomerular pathophysiology is associated with mRNA alternative splicing polyadenylation of genes critical slit diaphragm components regulate filtration barrier. Methods: damage, accompanied was induced puromycin-aminonucleoside or adriamycin mimic human minimal change disease FSGS, respectively, RNA-seq analyses performed. Alternatively spliced...
Recent findings revealed pivotal roles for eosinophils in protection against parasitic and viral infections, as well modulation of adaptive immune responses the gastric mucosa. However, known effects within respiratory tract remain predominantly pathological, associated with allergy asthma. Simulating natural infections mice, we examined how efficient well-adapted pathogens can block eosinophil functions that contribute to response. Bordetella bronchiseptica, a pathogen mouse, uses sigma...
Adriamycin (ADR)-induced nephropathy remains the leading model to study human primary focal segmental glomerulosclerosis (FSGS), a common pathway for podocyte damage and glomerular loss of function that leads chronic kidney disease. However, use this reverse genetics is limited by historical categorization C57BL/6 mice as an ADR-resistant strain, which also most genetically modified strain. Additionally, conflicting reports exist utilizing ADR-nephrosis due lack understanding substrain...
Concentrative nucleoside transporters (CNTs) are active influx systems, but their in vivo roles poorly defined. By generating CNT1 knockout (KO) mice, here we identify a role of the renal reabsorption nucleosides. Deletion mice increases urinary excretion endogenous pyrimidine nucleosides with compensatory alterations purine metabolism. In addition, KO exhibits high analog gemcitabine (dFdC), which results poor tumor growth control harboring syngeneic pancreatic tumors. Interestingly,...
Glomerular disease manifests as nephrotic syndrome (NS) with high proteinuria and comorbidities, is frequently refractory to standard treatments. We hypothesized that a selective modulator of PPARγ, GQ-16, will provide therapeutic advantage over traditional PPARγ agonists for NS treatment. demonstrate in pre-clinical model reduced pioglitazone 64%, robustly GQ-16 81% nephrosis, comparable controls. Although both restore glomerular-Nphs1, hepatic-Pcsk9 serum-cholesterol, only restores...
Background: Glomerular disease, often characterized by podocyte injury and proteinuria, can lead to chronic kidney disease end stage disease. We hypothesized that the glomerular pathophysiology is associated with alternative mRNA processing, such as splicing (AS) polyadenylation (APA), of genes especially critical podocyte, slit diaphragm matrix regulate filtration barrier. Methods: damage, accompanied hypoalbuminemia hypercholesterolemia was induced puromycin aminonucleoside (PAN) or...
Podocytes are highly differentiated epithelial cells, and their structural functional integrity is compromised in a majority of glomerular renal diseases, leading to proteinuria, chronic kidney disease, failure. Traditional agonists (e.g., pioglitazone) selective modulators GQ-16) peroxisome-proliferator-activated-receptor-γ (PPARγ) reduce proteinuria animal models disease protect podocytes from injury via PPARγ activation. This indicates pivotal role for maintaining function through...
Abstract Podocytes are highly differentiated epithelial cells, and their structural functional integrity is compromised in a majority of glomerular renal diseases, leading to proteinuria, chronic kidney disease, failure. Traditional agonists (e.g., pioglitazone) selective modulators GQ-16) peroxisome-proliferator-activated-receptor-γ (PPARγ) reduce proteinuria animal models disease protect podocytes from injury via PPARγ activation. This indicates pivotal role for maintaining function...